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Complete genome sequence of pleioblastus mosaic virus, a distinct member of the genus Potyvirus

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Abstract

Pleioblastus mosaic virus (PleMV) is a tentative member of the genus Potyvirus in the family Potyviridae and was discovered in bamboo with mosaic symptoms in Tokyo, Japan. Since no information on the genome sequence of PleMV has been reported, its taxonomic position has long been uncertain. Here, we report the first complete genome sequences of two distinct PleMV isolates. Excluding the 3′-terminal poly(A) tail, their genomic RNA sequences consist of 9,634 and 9,643 nucleotides (nt); both contain a large open reading frame (ORF) encoding a polyprotein and a small ORF termed PIPO. The large ORFs of the two isolates share 79.2% and 87.6% sequence identity at the nucleotide (nt) and amino acid (aa) level, respectively, and were found to have the highest nt and aa sequence identity (69.0% and 69.9%) to the potyvirus johnsongrass mosaic virus (JGMV). Phylogenetic analysis showed that PleMV is most closely related to JGMV but forms its own clade. These results suggest that PleMV is a distinct member of the genus Potyvirus.

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Availability of data and material

The complete genome sequence of PleMV has been deposited in the DNA Data Bank of Japan under accession numbers LC573287 and LC573288.

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Funding

This work was supported by the Japan Society for the Promotion of Science (JSPS) through Grants-in-Aid for Scientific Research (grants 19K23660, 20J23036, 17H03770, and 19K15805).

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Author notes

  1. Tetsuya Yoshida

    Present address: Plant and Microbial Research Unit, Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

Authors and Affiliations

  1. Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Kosuke Katsu, Takamichi Nijo, Tetsuya Yoshida, Masanobu Nishikawa, Akio Miyazaki, Kensaku Maejima, Shigetou Namba & Yasuyuki Yamaji

  2. Faculty of Food and Agricultural Sciences, Fukushima University, 1 Kanayagawa, Fukushima, Fukushima, 960-1296, Japan

    Yukari Okano

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  1. Kosuke Katsu
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Correspondence to Yasuyuki Yamaji.

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Katsu, K., Nijo, T., Yoshida, T. et al. Complete genome sequence of pleioblastus mosaic virus, a distinct member of the genus Potyvirus. Arch Virol 166, 645–649 (2021). https://doi.org/10.1007/s00705-020-04916-9

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  • DOI: https://doi.org/10.1007/s00705-020-04916-9

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