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Development and validation of a solid-phase competition ELISA based on virus-like particles of foot-and-mouth disease virus serotype A for antibody detection

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Abstract

Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious epidemic disease, which is controlled primarily by prophylactic vaccination and serological monitoring after vaccination. Here, we have developed a solid-phase competition ELISA (SPCE) method based on virus-like particles (VLPs) of FMDV serotype A. The use of VLPs in the SPCE assay as a replacement for inactivated FMDV provides a high level of biosafety. The SPCE showed high concordance rates when compared with the virus neutralization test and liquid-phase blocking ELISA for testing clinical serum samples and successive serological monitoring (kappa = 0.925). Thus, this SPCE is an alternative method for post-immunization detection of antibodies against FMDV serotype A, with high specificity and sensitivity.

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References

  1. Araujo JP, Montassier HJ, Pinto AA (1996) Liquid-phase blocking sandwich enzyme-linked immunosorbent assay for detection of antibodies against foot-and-mouth disease virus in water buffalo sera. Am J Vet Res 57:840–843

    CAS  PubMed  Google Scholar 

  2. Biswal JK, Bisht P, Mohapatra JK, Ranjan R, Sanyal A, Pattnaik B (2015) Application of a recombinant capsid polyprotein (P1) expressed in a prokaryotic system to detect antibodies against foot-and-mouth disease virus serotype O. J Virol Methods 215–216:45–51

    Article  Google Scholar 

  3. Chen HT, Peng YH, Zhang YG, Liu XT (2012) Detection of foot-and-mouth disease serotype O by ELISA using a monoclonal antibody. Hybridoma (Larchmt) 31:462–464

    Article  Google Scholar 

  4. Dong YM, Zhang GG, Huang XJ, Chen L, Chen HT (2015) Promising MS2 mediated virus-like particle vaccine against foot-and-mouth disease. Antiviral Res 117:39–43

    Article  CAS  Google Scholar 

  5. Feng X, Ma JW, Sun SQ, Guo HC, Yang YM, Jin Y, Zhou GQ, He JJ, Guo JH, Qi SY, Lin M, Cai H, Liu XT (2016) Quantitative detection of the foot-and-mouth disease virus serotype O 146S antigen for vaccine production using a double-antibody sandwich ELISA and nonlinear standard curves. PLoS ONE 11:e0149569

    Article  Google Scholar 

  6. Ferris NP, Grazioli S, Hutchings GH, Brocchi E (2011) Validation of a recombinant integrin alphavbeta6/monoclonal antibody based antigen ELISA for the diagnosis of foot-and-mouth disease. J Virol Methods 175:253–260

    Article  CAS  Google Scholar 

  7. Guo HC, Sun SQ, Jin Y, Yang SL, Wei YQ, Sun DH, Yin SH, Ma JW, Liu ZX, Guo JH, Luo JX, Yin H, Liu XT, Liu DX (2013) Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle. Vet Res 44:48

    Article  CAS  Google Scholar 

  8. Hamblin C, Barnett IT, Hedger RS (1986) A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. I. Development and method of ELISA. J Immunol Methods 93:115–121

    Article  CAS  Google Scholar 

  9. Jamal SM, Belsham GJ (2013) Foot-and-mouth disease: past, present and future. Vet Res 44:116

    Article  Google Scholar 

  10. Jin H, Xiao W, Xiao C, Yu Y, Kang Y, Du X, Wei X, Wang B (2007) Protective immune responses against foot-and-mouth disease virus by vaccination with a DNA vaccine expressing virus-like particles. Viral Immunol 20:429–440

    Article  CAS  Google Scholar 

  11. Li H, Li Z, Xie Y, Qin X, Qi X, Sun P, Bai X, Ma Y, Zhang Z (2016) Novel chimeric foot-and-mouth disease virus-like particles harboring serotype O VP1 protect guinea pigs against challenge. Vet Microbiol 183:92–96

    Article  CAS  Google Scholar 

  12. Liu W, Yang B, Wang M, Liang W, Wang H, Yang D, Ma W, Zhou G, Yu L (2017) Identification of a conserved conformational epitope in the VP2 protein of foot-and-mouth disease virus. Arch Virol 162:1877–1885

    Article  CAS  Google Scholar 

  13. Liu W, Yang B, Wang M, Wang H, Yang D, Ma W, Zhou G, Yu L (2017) Identification of a conformational neutralizing epitope on the VP1 protein of type A foot-and-mouth disease virus. Res Vet Sci 115:374–381

    Article  CAS  Google Scholar 

  14. Liu X, Fang Y, Zhou P, Lu Y, Zhang Q, Xiao S, Dong Z, Pan L, Lv J, Zhang Z, Zhang Y, Wang Y (2017) Chimeric virus-like particles elicit protective immunity against serotype O foot-and-mouth disease virus in guinea pigs. Appl Microbiol Biotechnol 101:4905–4914

    Article  CAS  Google Scholar 

  15. Mackay DK, Bulut AN, Rendle T, Davidson F, Ferris NP (2001) A solid-phase competition ELISA for measuring antibody to foot-and-mouth disease virus. J Virol Methods 97:33–48

    Article  CAS  Google Scholar 

  16. Morioka K, Fukai K, Yoshida K, Yamazoe R, Onozato H, Ohashi S, Tsuda T, Sakamoto K (2009) Foot-and-mouth disease virus antigen detection enzyme-linked immunosorbent assay using multiserotype-reactive monoclonal antibodies. J Clin Microbiol 47:3663–3668

    Article  Google Scholar 

  17. OIE (2018) Manual of diagnostic tests and vaccines for terrestrial animals, 8th edn. World Organization for Animal Health (OIE), Paris

  18. Paiba GA, Anderson J, Paton DJ, Soldan AW, Alexandersen S, Corteyn M, Wilsden G, Hamblin P, MacKay DK, Donaldson AI (2004) Validation of a foot-and-mouth disease antibody screening solid-phase competition ELISA (SPCE). J Virol Methods 115:145–158

    Article  CAS  Google Scholar 

  19. Ran X, Yang Z, Bai M, Zhang Y, Wen X, Guo H, Sun S (2019) Development and validation of a competitive ELISA based on bacterium-original virus-like particles of serotype O foot-and-mouth disease virus for detecting serum antibodies. Appl Microbiol Biotechnol 103:3015–3024

    Article  CAS  Google Scholar 

  20. Roeder PL, Le Blanc Smith PM (1987) Detection and typing of foot-and-mouth disease virus by enzyme-linked immunosorbent assay: a sensitive, rapid and reliable technique for primary diagnosis. Res Vet Sci 43:225–232

    Article  CAS  Google Scholar 

  21. Spitteler MA, Romo A, Magi N, Seo MG, Yun SJ, Barroumeres F, Regulier EG, Bellinzoni R (2019) Validation of a high performance liquid chromatography method for quantitation of foot-and-mouth disease virus antigen in vaccines and vaccine manufacturing. Vaccine 37:5288–5296

    Article  CAS  Google Scholar 

  22. Tsang VC, Greene RM, Pilcher JB (1995) Optimization of the covalent conjugating procedure (NaIO4) of horseradish peroxidase to antibodies for use in enzyme-linked immunosorbent assay. J Immunoassay 16:395–418

    Article  CAS  Google Scholar 

  23. Wang H, Zhao L, Li W, Zhou G, Yu L (2011) Identification of a conformational epitope on the VP1 G-H Loop of type Asia1 foot-and-mouth disease virus defined by a protective monoclonal antibody. Vet Microbiol 148:189–199

    Article  CAS  Google Scholar 

  24. Xiao Y, Chen HY, Wang Y, Yin B, Lv C, Mo X, Yan H, Xuan Y, Huang Y, Pang W, Li X, Yuan YA, Tian K (2016) Large-scale production of foot-and-mouth disease virus (serotype Asia1) VLP vaccine in Escherichia coli and protection potency evaluation in cattle. BMC Biotechnol 16:56

    Article  Google Scholar 

  25. Yang Y, Zhao Q, Li Z, Sun L, Ma G, Zhang S, Su Z (2017) Stabilization study of inactivated foot and mouth disease virus vaccine by size-exclusion HPLC and differential scanning calorimetry. Vaccine 35:2413–2419

    Article  CAS  Google Scholar 

  26. Zhang Y, Yao Y, Gao X, Wang Y, Jia X, Xiao Y, Wang T, Li X, Tian K (2016) Development of a neutralizing monoclonal antibody against porcine epidemic diarrhea virus S1 protein. Monoclon Antib Immunodiagn Immunother 35:37–40

    Article  CAS  Google Scholar 

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Acknowledgements

This study was supported by a grant from the Special Project of Industrial Cluster in Self-Created Zone in Zhengzhou, Luoyang, and Xinxiang Cities (181200211700) and Luoyang Heluo Talent Plan.

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Authors and Affiliations

  1. National Research Center for Veterinary Medicine, No. 3 Cuiwei Road, High-Tech District, Luoyang, 471003, Henan, People’s Republic of China

    Yunjing Zhang, He Yan, Yali Yao, Suling Zhang, Yan Xiao, Xin Xu, Baicheng Huang & Kegong Tian

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  1. Yunjing Zhang
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  2. He Yan
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  3. Yali Yao
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  4. Suling Zhang
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Correspondence to Baicheng Huang or Kegong Tian.

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Zhang, Y., Yan, H., Yao, Y. et al. Development and validation of a solid-phase competition ELISA based on virus-like particles of foot-and-mouth disease virus serotype A for antibody detection. Arch Virol 165, 1641–1646 (2020). https://doi.org/10.1007/s00705-020-04641-3

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  • DOI: https://doi.org/10.1007/s00705-020-04641-3

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