Abstract
Foot-and-mouth disease (FMD) is an acute and highly contagious disease caused by foot-and-mouth disease virus (FMDV) that can affect cloven-hoofed animal species, leading to severe economic losses worldwide. Therefore, the development of a safe and effective new vaccine to prevent and control FMD is both urgent and necessary. In this study, we developed a chimeric virus-like particle (VLP) vaccine candidate for serotype O FMDV and evaluated its protective immunity in guinea pigs. Chimeric VLPs were formed by the antigenic structural protein VP1 from serotype O and segments of the viral capsid proteins (VP2, VP3, and VP4) from serotype A. The chimeric VLPs elicited significant humoral and cellular immune responses with a higher level of anti-FMDV antibodies and cytokines than the control group. Furthermore, four of the five guinea pigs vaccinated with the chimeric VLPs were completely protected against challenge with 100 50% guinea pig infectious doses (GPID50) of the virulent FMDV strain O/MAY98. These data suggest that chimeric VLPs are potential candidates for the development of new vaccines against FMDV.
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Acknowledgments
This work was supported by grants from the National Pig Industrial System (CARS-36-068), the Special Fund for Agroscientific Research in the Public Interest (201203039), the National Key Research and Development Program (2016YFD0501505), and the Co-ordination Program of Lanzhou Veterinary Research Institute (Y2016CG23).
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All guinea pig experiments were performed in a biosafety level 3 laboratory at Lanzhou Veterinary Research Institute (LVRI), Chinese Academy of Agricultural Sciences (CAAS). All of the animal protocols were approved by the Institutional Animal Use and Care Committee of the CAAS. All guinea pigs used in the present study were humanely bred during the experiment and euthanized at the end of the experiment.
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Liu, X., Fang, Y., Zhou, P. et al. Chimeric virus-like particles elicit protective immunity against serotype O foot-and-mouth disease virus in guinea pigs. Appl Microbiol Biotechnol 101, 4905–4914 (2017). https://doi.org/10.1007/s00253-017-8246-0
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DOI: https://doi.org/10.1007/s00253-017-8246-0