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Complete genome sequence of a novel grapevine-infecting member of the genus Polerovirus, grapevine polerovirus 1

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Abstract

Double-stranded RNAs and total RNAs purified from grapevine (Vitis vinifera) phloem scrapings of two varieties held in the INRAE (France) grapevine germplasm collection were analyzed by high-throughput sequencing. BLAST annotation revealed contigs with homology to Polerovirus genus members. The full genome sequence of one isolate (KT) was determined (5651 nucleotides [nt]), and a partial sequence representing about half of the genome was assembled for a second isolate (KS) that was found to share 95% nt sequence identity with the KT isolate. The genome has a typical polerovirus organization, containing six open reading frames (ORFs) as well as a putative additional ORF3a. Based on genome organization and phylogenetic relationships, the new virus belongs to the genus Polerovirus but, similar to the recently described persimmon polerovirus 1, is characterized by a highly divergent coat-protein/readthrough domain. Considering the species demarcation criteria for the family Luteoviridae, these two isolates, together with a closely related sequence recently deposited in the GenBank database (LC507098), represent a new Polerovirus species for which the name “Grapevine polerovirus 1” is proposed.

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Acknowledgements

The authors thank the INRAE GenoToul Platform (Toulouse, France) for high-throughput sequencing and Sandrine Dedet and Cécile Marchal (CRB Vigne, INRAE, Marseillan-Plage, France) for help in preparing the grapevine materials used.

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Authors and Affiliations

  1. UMR 1332 Biologie du Fruit et Pathologie, INRAE, Univ. Bordeaux, 72 Avenue Edouard Bourlaux, CS20032, 33882, Villenave d’Ornon cedex, France

    T. Candresse, A. Marais, C. Faure & M. Lefebvre

  2. UMR AGAP, INRAE, Montpellier SupAgro, TA A-108/03 Avenue Agropolis, 34398, Montpellier Cedex 5, France

    T. Lacombe & J. M. Boursiquot

  3. Centre de Ressources Biologiques de la Vigne, INRAE, 34340, Marseillan-Plage, France

    T. Lacombe & J. M. Boursiquot

Authors
  1. T. Candresse
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  2. A. Marais
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  3. C. Faure
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  4. M. Lefebvre
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  5. T. Lacombe
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  6. J. M. Boursiquot
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Correspondence to T. Candresse.

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This manuscript presents original work that does not involve studies with humans or animals.

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Handling Editor: Robert H.A. Coutts.

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The nucleotide sequences reported in this manuscript have been deposited in the GenBank database under accession numbers MT008025-26.

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Candresse, T., Marais, A., Faure, C. et al. Complete genome sequence of a novel grapevine-infecting member of the genus Polerovirus, grapevine polerovirus 1. Arch Virol 165, 1683–1685 (2020). https://doi.org/10.1007/s00705-020-04640-4

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  • DOI: https://doi.org/10.1007/s00705-020-04640-4

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