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Characterization of a novel echovirus 21 strain isolated from a healthy child in China in 2013

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Abstract

Echovirus 21 (E21) belongs to the species Enterovirus B, whose members are frequently associated with acute flaccid paralysis. E21 strain 553/YN/CHN/2013 was isolated from a healthy child in Yunnan, China, in 2013. This is the first report of the complete genome sequence of E21 in China. This strain shared 81.7% nucleotide sequence identity and 96.8% amino acid sequence identity with the E21 prototype strain Farina. Although strain 553/YN/CHN/2013 belongs to the E21 serotype, the only similarity to the E21 strain was in the VP1 region, as other genomic regions, including VP2–VP4, were more similar to other EV-B members. Recombination analysis showed evidence of recombination events between E21 and other EV-B viruses. E21 strain 553/YN/CHN/2013 failed to infect suckling mice via intracerebral injection. Surveillance of E21 is very important to help forecast the potential of emerging E21 outbreaks and related diseases.

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Acknowledgements

This work was supported by the Basic Research Projects of Yunnan Province, China (Grant Number 2017FA006) and CAMS Innovation Fund for Medical Sciences (CIFMS) (Grant Number 2016-I2M-3-026).

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Author notes

  1. Hongbo Liu and Shanri Cong have contributed equally to this study and are co-first authors.

Authors and Affiliations

  1. Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College (CAMS and PUMC), 935 Jiao Ling Road, Kunming, 650118, Yunnan, People’s Republic of China

    Hongbo Liu, Shanri Cong, Danhan Xu, Keqin Lin, Xiaoqin Huang, Hao Sun, Zhaoqing Yang & Shaohui Ma

  2. Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Disease, Kunming, 650118, People’s Republic of China

    Hongbo Liu, Shanri Cong, Danhan Xu, Keqin Lin, Xiaoqin Huang, Hao Sun, Zhaoqing Yang & Shaohui Ma

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  1. Hongbo Liu
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Correspondence to Zhaoqing Yang or Shaohui Ma.

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Ethical approval

All experimental protocols involving human samples were approved by the Human Ethics Commission of the Institute of Medical Biology, Chinese Academy of Medical Sciences. The use of experimental animals was approved by the Animal Ethics Committee of the Institute of Medical Biology, Chinese Academy of Medical Sciences (approval number DWSP201804001).

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Liu, H., Cong, S., Xu, D. et al. Characterization of a novel echovirus 21 strain isolated from a healthy child in China in 2013. Arch Virol 165, 757–760 (2020). https://doi.org/10.1007/s00705-019-04506-4

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  • DOI: https://doi.org/10.1007/s00705-019-04506-4

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