Abstract
Forty-five papaya samples showing severe leaf curl symptoms were tested by PCR with a degenerate primer set for virus species in the genus Begomovirus. Of these, 29 were positive for tomato leaf curl Bangladesh virus (ToLCBV). The complete genome sequences of ToLCBV (GenBank accession no. MH380003) and its associated tomato leaf curl betasatellite (ToLCB) (MH397223) from papaya isolate Gaz17-Pap were determined and characterized. Defective betasatellites were found in ToLCBV-positive papaya isolates Gaz19-Pap, Gaz20-Pap and Gaz21-Pap. This study confirmed that papaya is a host of ToLCBV, ToLCB, and other defective and recombinant DNA satellites in Bangladesh.
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Acknowledgements
This work was supported by the United States Agency for International Development (Feed the Future initiative–CGIAR fund- BFS-G-1100002 and the predecessor fund of the Food Security and Crisis Mitigation II grant- EEM-G-0004-00013), the USDA National Institute of Food and Agriculture, Hatch HAW09025-H (1001478) and the USDA-Agricultural Research Service (58-5320-4-012).
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Hamim, I., Borth, W.B., Melzer, M.J. et al. Occurrence of tomato leaf curl Bangladesh virus and associated subviral DNA molecules in papaya in Bangladesh: molecular detection and characterization. Arch Virol 164, 1661–1665 (2019). https://doi.org/10.1007/s00705-019-04235-8
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DOI: https://doi.org/10.1007/s00705-019-04235-8