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Molecular characterization of Morogoro maize-associated virus, a nucleorhabdovirus detected in maize (Zea mays) in Tanzania

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Abstract

RNAtag-seq of maize samples collected in Tanzania revealed the presence of a previously undescribed nucleorhabdovirus, tentatively named “Morogoro maize-associated virus” (MMaV), in three samples. The MMaV genome is 12,185-12,187 nucleotides long and shares a 69-70% nucleotide sequence identity with taro vein chlorosis virus. Annotation of the genomes showed a typical nucleorhabdovirus gene organization. PCR was unable to detect the same virus in the remaining 35 samples collected in the region.

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Authors and Affiliations

  1. Agricultural Research Council-Biotechnology Platform, Onderstepoort, Private Bag X05, Pretoria, 0110, South Africa

    David Alan Read, Jonathan Featherston, David Jasper Gilbert Rees & Genevieve Dawn Thompson

  2. Agricultural Research Council-Plant Protection Research, Queenswood, Private Bag X134, Pretoria, 0001, South Africa

    Ronel Roberts

  3. Agricultural Research Council-Grain Crops, Private Bag X1251, Potchefstroom, 2520, South Africa

    Bradley Charles Flett & Kingstone Mashingaidze

  4. Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa

    Gerhard Pietersen

  5. Tanzania Agricultural Research Institute (TARI-Ilonga), Kilosa, Morogoro, Tanzania

    Barnabas Kiula

  6. Tanzania Agricultural Research Institute (TARI, Mikocheni), Dar es Salaam, Tanzania

    Alois Kullaya

  7. Nelson Mandela African Institution of Science and Technology (NM-AIST), Arusha, Tanzania

    Ernest R. Mbega

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  1. David Alan Read
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  2. Jonathan Featherston
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  3. David Jasper Gilbert Rees
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  4. Genevieve Dawn Thompson
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  6. Bradley Charles Flett
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  7. Kingstone Mashingaidze
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  9. Barnabas Kiula
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  10. Alois Kullaya
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  11. Ernest R. Mbega
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Correspondence to David Alan Read.

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Read, D.A., Featherston, J., Rees, D.J.G. et al. Molecular characterization of Morogoro maize-associated virus, a nucleorhabdovirus detected in maize (Zea mays) in Tanzania. Arch Virol 164, 1711–1715 (2019). https://doi.org/10.1007/s00705-019-04212-1

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  • DOI: https://doi.org/10.1007/s00705-019-04212-1

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