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Establishment of a rescue system for porcine parvovirus using a seamless cloning method

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Abstract

In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms of its biological properties. The generation of this PPV infectious clone provides a potentially powerful tool with which to elucidate the molecular pathogenesis of PPV.

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Acknowledgements

This work was supported by the Agricultural Science and Technology Innovation Program (ASTIP-IAS15), National Natural Science Foundation of China (NO. 31172349 & NO. 31172341) and the National Key Research and Development Program of China (No. 2016YFD0501003 & 2017YFD0502300).

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Author notes

  1. Lingling Zhang and Dou Gao contributed equally to this work.

Authors and Affiliations

  1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Lingling Zhang, Dou Gao & Shangjin Cui

  2. Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Technology of Beijing, Ministry of Agriculture, Beijing, 100193, China

    Lingling Zhang, Dou Gao & Shangjin Cui

  3. State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China

    Yongle Yu & Weiquan Liu

  4. China Institute of Veterinary Drugs Control, Beijing, 100081, China

    Yebing Liu

  5. Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Jinxiang Li

Authors
  1. Lingling Zhang
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  2. Dou Gao
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  3. Yongle Yu
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  4. Yebing Liu
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  5. Weiquan Liu
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  6. Jinxiang Li
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  7. Shangjin Cui
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Corresponding author

Correspondence to Shangjin Cui.

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All authors declare they have no conflict of interest.

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This article does not contain any studies with human participants and the animals studies was approved by the Science Research Department (in charge of animal welfare issue) of the Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (IAS-CAAS) (Beijing, China).

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Zhang, L., Gao, D., Yu, Y. et al. Establishment of a rescue system for porcine parvovirus using a seamless cloning method. Arch Virol 164, 1459–1467 (2019). https://doi.org/10.1007/s00705-019-04209-w

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  • DOI: https://doi.org/10.1007/s00705-019-04209-w

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