Abstract
Bacillus subtilis-infecting phage BSP38 was isolated from a sewage sample. Morphologically, BSP38 was found to be similar to members of the subfamily Spounavirinae, family Myoviridae. Its genome is 153,268 bp long with 41.8% G+C content and 254 putative open reading frames (ORFs) as well as six tRNAs. A distinguishing feature for this phage among the reported B. subtilis-infecting phages is the presence of an encoding ORF, putative tRNAHis guanylyltransferase-like protein. Genomic comparisons with the other reported phages strongly suggest that BSP38 should be considered a member of a new genus in the subfamily Spounavirinae.
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Acknowledgements
This work was supported by the National Research Foundation of Korea (Project no. NRF-2016R1A2B4006967) and by research funds of Chonbuk National University in 2018.
Funding
This study was funded by the National Research Foundation of Korea (Project no. NRF-2016R1A2B4006967) and by research funds of Chonbuk National University in 2018.
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Nucleotide sequence accession number
The whole genome sequence of Bacillus phage BSP38 was deposited in GenBank under accession number MH606185 (http://www.ncbi.nlm.nih.gov/nuccore/MH606185).
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Ghosh, K., Kim, KP. Complete nucleotide sequence analysis of a novel Bacillus subtilis-infecting phage, BSP38, possibly belonging to a new genus in the subfamily Spounavirinae. Arch Virol 164, 875–878 (2019). https://doi.org/10.1007/s00705-018-4110-5
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DOI: https://doi.org/10.1007/s00705-018-4110-5