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Complete genome sequence of C130_2, a novel myovirus infecting pathogenic Escherichia coli and Shigella strains

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Abstract

The genome sequence of a novel virulent bacteriophage, named “ C130_2”, that is morphologically a member of the family Myoviridae is reported. The 41,775-base-pair double-stranded DNA genome of C130_2 contains 59 ORFs but exhibits overall low sequence similarity to bacteriophage genomes for which sequences are publicly available. Phylogenetic analysis indicated that C130_2 represents a new phage type. C130_2 could be propagated well on enterohemorrhagic Escherichia coli (EHEC) O157:H7 and other pathogenic E. coli strains, as well as on strains of various Shigella species.

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Acknowledgements

This study was supported by the Hungarian National Research, Development and Innovative Office (NKFIH, Grant 124335), 7th EU Framework Programme PROMISE project (Project No. 265877) and by grants to the German Center of Infection Research (DZIF, site Giessen-Marburg-Langen), through the German Federal Ministry of Education and Research, Grant # TI06.001 and 8032808811 to TC. We thank Christina Gerstmann for excellent technical assistance. Domonkos Sváb was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences.

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Authors and Affiliations

  1. Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary

    Domonkos Sváb & István Tóth

  2. Institute of Medical Microbiology, Justus Liebig University Giessen and German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, Giessen, Germany

    Linda Falgenhauer & Trinad Chakraborty

  3. Central Facility for Microscopy, Helmholtz Centre for Infection Research, HZI, Braunschweig, Germany

    Manfred Rohde

Authors
  1. Domonkos Sváb
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  2. Linda Falgenhauer
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  4. Trinad Chakraborty
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  5. István Tóth
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Correspondence to István Tóth.

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Handling Editor: Tim Skern.

Electronic supplementary material

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Supplementary Table 1

Host spectrum and efficiency of plating (EOP) of bacteriophage C130_2. EOP values are given relative to the titre measured on E. coli K-12 MG1655 strain (XLSX 12 kb)

Supplementary Table 2

List of ORFs of phage C130_2 with assigned functions and protein homology searches. A PROSITE search was performed including motifs with high probability occurrences. A UniProt search was performed with narrowing down to viral proteins (XLSX 471 kb)

Supplementary Table 3

List of ORFs of phages C130_2, IME_EC2 and vB_KpnS_IME279, with corresponding ORFs above 75% similarity highlighted in blue (XLSX 14 kb)

Supplementary Fig. 1

BLAST-based comparison of the whole genomes of bacteriophages C130_2, IME_EC2 and vB_KpnS_IME279. Orange arrows represent genes, numbers on C130_2 genes correspond to ORF numbers in Supplementary Tables 2 and 3. Regions showing > 50% similarity are interconnected with grey lines (TIFF 1220 kb)

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Sváb, D., Falgenhauer, L., Rohde, M. et al. Complete genome sequence of C130_2, a novel myovirus infecting pathogenic Escherichia coli and Shigella strains. Arch Virol 164, 321–324 (2019). https://doi.org/10.1007/s00705-018-4042-0

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  • DOI: https://doi.org/10.1007/s00705-018-4042-0

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