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Archives of Virology

, Volume 163, Issue 3, pp 707–712 | Cite as

Genetic analysis of three porcine bocaparvoviruses and identification of a natural recombinant breakpoint in NS1

Brief Report
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Abstract

In this study, we obtained the whole genomes of three porcine bocaparvovirus (PBoV) strains (GD6, GD10, and GD23) by polymerase chain reaction. Sequence analysis showed that all three field strains belonged to PBoV group 3 (G3). The phylogenetic trees based on NS1, NP1, and VP1 differed to the extent that these PBoVs were potentially more closely related to bocaparvoviruses known to infect other animals than to other PBoVs. GD6, GD10, and GD23 all included the conserved sequences YLGPF and HDXXY, with known phospholipase A2 activity. Using recombination-detection software we identified a natural recombinant breakpoint in the NS1 region of PBoV G3. The results of this study will further the epidemiological characterization of PBoVs.

Notes

Acknowledgements

This work was financially supported by grants from the Huizhou City Science and Technology Bureau (2016G0414029).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2017_3606_MOESM1_ESM.pdf (22 kb)
Supplementary material 1 (PDF 22 kb)
705_2017_3606_MOESM2_ESM.pdf (363 kb)
Figure S1: Phylogenetic analysis of PBoVs GD6, GD10, and GD23 when compared with other BoVs, based on ORF2. Figure S2: Phylogenetic analysis of PBoVs GD6, GD10, and GD23 when compared with other BoVs, based on ORF3. Figure S3: The putative phospholipase A2 motif in BoVs. The HDXXY and YLGPF motifs of PLA2 are indicated by a blue grid (PDF 362 kb)

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Copyright information

© Springer-Verlag GmbH Austria 2017

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyJilin Agricultural UniversityChangchunPeople’s Republic of China
  2. 2.Huizhou Entry-Exit Inspection and Quarantine BureauHuizhouPeople’s Republic of China
  3. 3.Animal Disease Control and Prevention of Heilongjiang ProvinceHarbinPeople’s Republic of China
  4. 4.Jilin Entry-Exit Inspection and Quarantine BureauChangchunPeople’s Republic of China
  5. 5.Guangdong Entry-Exit Inspection and Quarantine BureauGuangZhouPeople’s Republic of China

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