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Genetic analysis of three porcine bocaparvoviruses and identification of a natural recombinant breakpoint in NS1

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Abstract

In this study, we obtained the whole genomes of three porcine bocaparvovirus (PBoV) strains (GD6, GD10, and GD23) by polymerase chain reaction. Sequence analysis showed that all three field strains belonged to PBoV group 3 (G3). The phylogenetic trees based on NS1, NP1, and VP1 differed to the extent that these PBoVs were potentially more closely related to bocaparvoviruses known to infect other animals than to other PBoVs. GD6, GD10, and GD23 all included the conserved sequences YLGPF and HDXXY, with known phospholipase A2 activity. Using recombination-detection software we identified a natural recombinant breakpoint in the NS1 region of PBoV G3. The results of this study will further the epidemiological characterization of PBoVs.

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Acknowledgements

This work was financially supported by grants from the Huizhou City Science and Technology Bureau (2016G0414029).

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Author notes

  1. Yu Zhou and Jia Xu these authors contributed equally to this paper.

Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, 130118, Jilin Province, People’s Republic of China

    Yu Zhou & Ai-Dong Qian

  2. Huizhou Entry-Exit Inspection and Quarantine Bureau, No. 277 Zhongkai Road, Huizhou, 516006, Guangdong Province, People’s Republic of China

    Yu Zhou & Shi-Kang Zhu

  3. Animal Disease Control and Prevention of Heilongjiang Province, No. 243 Haping Road, Xiangfang District, Harbin, 150069, People’s Republic of China

    Jia Xu

  4. Jilin Entry-Exit Inspection and Quarantine Bureau, No. 361 Puyang Street, Changchun, 130062, Jilin Province, People’s Republic of China

    Qing-Feng Meng

  5. Guangdong Entry-Exit Inspection and Quarantine Bureau, No. 66 Huacheng Road, GuangZhou, 510623, Guangdong Province, People’s Republic of China

    Zhi-Xiong Lin & Ru Chen

Authors
  1. Yu Zhou
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  2. Jia Xu
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  3. Shi-Kang Zhu
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  4. Qing-Feng Meng
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  5. Zhi-Xiong Lin
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  6. Ru Chen
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  7. Ai-Dong Qian
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Corresponding authors

Correspondence to Ru Chen or Ai-Dong Qian.

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The authors declare no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Zhenhai Chen.

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Supplementary material 1 (PDF 22 kb)

705_2017_3606_MOESM2_ESM.pdf

Figure S1: Phylogenetic analysis of PBoVs GD6, GD10, and GD23 when compared with other BoVs, based on ORF2. Figure S2: Phylogenetic analysis of PBoVs GD6, GD10, and GD23 when compared with other BoVs, based on ORF3. Figure S3: The putative phospholipase A2 motif in BoVs. The HDXXY and YLGPF motifs of PLA2 are indicated by a blue grid (PDF 362 kb)

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Zhou, Y., Xu, J., Zhu, SK. et al. Genetic analysis of three porcine bocaparvoviruses and identification of a natural recombinant breakpoint in NS1. Arch Virol 163, 707–712 (2018). https://doi.org/10.1007/s00705-017-3606-8

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  • DOI: https://doi.org/10.1007/s00705-017-3606-8

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