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Complete genome sequence of a novel lytic bacteriophage isolated from Ralstonia solanacearum

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Abstract

A lytic podophage RSPI1 was isolated from tobacco field soil collected in Fujian Province, South China using host bacterium Ralstonia solanacearum Tb15-14. Whole genome sequencing of this phage was performed using the high-throughput Ion Torrent PGM Sequencer. The complete genome of RSPI1 was 43,211 bp in length with a mean DNA G + C content of 61.5%. A total of 48 open reading frames were identified with lengths ranging from 132 bp to 5,061 bp, of which, 11, 12 and 25 were identified as functional, structural and unknown genes, respectively. A BLAST analysis revealed that this phage genome had a query cover of 78–79% and a highest identity of 84% with four podophages that infect Burkholderia pseudomallei. Two neighbor-joining phylogenetic trees were constructed using phage DNA polymerase I and tail fiber protein sequences and showed that this phage is closely related to Burkholderia phage Bp-AMP1, and also related to several phages that infect Ralstonia solanacearum. These findings indicate that RSPI1 is a novel phage that infects the notorious plant pathogen Ralstonia solanacearum.

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Acknowledgements

The authors thank the members of Professor Yigang Tong’s group in the Beijing Institute of Microbiology and Epidemiology for help conducting sequence analysis.

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Authors and Affiliations

  1. Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China

    Jingfang Su, Junjie Liu & Guanghua Wang

  2. Mudanjiang Tobacco Research Institute, Heilongjiang Branch of China National Tobacco Corporation, Mudangjiang, 157011, China

    Hongwei Sun & Zhaokui Guo

  3. Virus-free Seedling, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China

    Guoquan Fan

  4. Tobacco Agricultural Institute of Fujian Tobacco Monopoly Bureau, Fuzhou, 350003, China

    Gang Gu

Authors
  1. Jingfang Su
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  2. Hongwei Sun
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  3. Junjie Liu
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  4. Zhaokui Guo
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  5. Guoquan Fan
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  6. Gang Gu
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  7. Guanghua Wang
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Corresponding author

Correspondence to Guanghua Wang.

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Funding

This study was funded by the Key Project of Science and Technology of China National Tobacco Corporation (Grant number 110201402014).

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Horst Neve.

Nucleotide sequence accession numbers:

The complete genome sequence of phage RSPI1 was deposited into the GenBank database under accession number KY464836.

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Supplementary material 1 (DOCX 29 kb)

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Su, J., Sun, H., Liu, J. et al. Complete genome sequence of a novel lytic bacteriophage isolated from Ralstonia solanacearum . Arch Virol 162, 3919–3923 (2017). https://doi.org/10.1007/s00705-017-3555-2

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  • DOI: https://doi.org/10.1007/s00705-017-3555-2

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