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Characterization of reassortant H1-subtype avian influenza viruses isolated from poultry in Zhejiang Province in China from 2013 to 2015

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Abstract

From 2013 to 2015, 32 H1-subtype avian influenza viruses (AIVs), H1N2 (n = 12), H1N3 (n = 14), H1N4 (n = 4) and H1N9 (n = 2), were isolated from poultry in Zhejiang Province in eastern China. These strains were characterized by whole-genome sequencing with subsequent phylogenetic analysis and genetic comparison. Phylogenetic analysis of all eight viral genes showed that these strains clustered in the AIV Eurasian lineage. These strains were found to be minimally pathogenic in mice and were able to replicate in mice without prior adaptation. Continued surveillance is needed, considering the important role of poultry in AIV reassortment.

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Authors and Affiliations

  1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China

    Haibo Wu, Xiuming Peng, Fumin Liu, Linfang Cheng & Nanping Wu

  2. Department of Emergency, The First Affiliated Hospital of Zhejiang Chinese Medical University, 310006, Hangzhou, China

    Rufeng Lu

Authors
  1. Haibo Wu
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  2. Rufeng Lu
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  3. Xiuming Peng
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  4. Fumin Liu
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  5. Linfang Cheng
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  6. Nanping Wu
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Corresponding author

Correspondence to Nanping Wu.

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Funding

This study was funded by grants from the National Science Foundation of the People’s Republic of China (81502852), Zhejiang Provincial Natural Science Foundation of China (LY15H190003), and the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases (Nos. 2016ZZ03 and 2017ZZ10).

Conflict of interest

The authors declare that they had no conflict of interest.

Ethical approval

The animal experiment was approved by the First Affiliated Hospital, School of Medicine, Zhejiang University (No. 2015-15).

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Wu, H., Lu, R., Peng, X. et al. Characterization of reassortant H1-subtype avian influenza viruses isolated from poultry in Zhejiang Province in China from 2013 to 2015. Arch Virol 162, 3493–3500 (2017). https://doi.org/10.1007/s00705-017-3487-x

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  • DOI: https://doi.org/10.1007/s00705-017-3487-x

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