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Genetic characterization of subtype H1 avian influenza viruses isolated from live poultry markets in Zhejiang Province, China, in 2011

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Abstract

Nine avian influenza A viruses (AIVs), H1N2 (n = 2) and H1N3 (n = 7), were isolated from domestic ducks in live poultry markets in Zhejiang Province, Eastern China, in 2011. All viruses were characterized by whole genome sequencing with subsequent phylogenetic analysis and genetic comparison. Phylogenetic analysis of all eight viral genes showed that the viruses clustered in the Eurasian lineage of influenza A viruses. The hemagglutinin cleavage site of all viruses displayed features of a monobasic cleavage site. Although there was no evidence of re-assortment in subtype H1 AIVs among the avian species and mammalian hosts in this study, continued surveillance is needed considering the important role of the domestic duck in the dissemination and re-assortment of AIVs.

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Acknowledgments

This study was supported by Grants from the National Science Foundation of the People’s Republic of China (30872163), National Key Technologies R&D Programme for the 12th Five-Year Plan of China (2012ZX1000-004-005) and the State Key Laboratory of independent task (No. 2010ZZ04).

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Authors and Affiliations

  1. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China

    Hai-bo Wu & Nan-ping Wu

  2. Institute of Bioengineering, Zhejiang Academy of Medical Sciences, 182 Tianmushan Road, Hangzhou, 310013, Zhejiang, China

    Hai-bo Wu, Chao-tan Guo, En-kang Wo, Jin-biao You, Yi-ting Wang & Qiao-gang Wang

  3. Department of Emergency, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310006, Zhejiang, China

    Ru-feng Lu

  4. Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, Zhejiang, China

    Li-hua Xu

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  1. Hai-bo Wu
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Correspondence to Chao-tan Guo or Nan-ping Wu.

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Wu, Hb., Guo, Ct., Lu, Rf. et al. Genetic characterization of subtype H1 avian influenza viruses isolated from live poultry markets in Zhejiang Province, China, in 2011. Virus Genes 44, 441–449 (2012). https://doi.org/10.1007/s11262-012-0716-y

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  • DOI: https://doi.org/10.1007/s11262-012-0716-y

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