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Detection and genotyping of bovine leukemia virus in Mexican cattle

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Abstract

Bovine leukemia virus (BLV) was detected and genotyped in a population of 201 dairy cattle from central Mexico. Using a commercial indirect enzyme-linked immunosorbent assay (iELISA) kit, 118 polymerase chain reaction (PCR)-positive and BLV antibody-positive samples were identified; the concordance between tests was substantial. A phylogenetic study of 27 partial sequences of the env gene gp30 was performed. Four mutations were detected involving the PXXP motif in the cytoplasmic domain of the transmembrane protein. This study provided evidence of the efficacy of PCR for the detection of BLV and demonstrated the presence of genotype 1 BLV in Mexico.

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Authors and Affiliations

  1. Facultad de Estudios Superiores Cuautitlán, Veterinary Medicine, Virology, Genetics and Molecular Biology Laboratory, Universidad Nacional Autónoma de México, Campus 4. Km 2.5 Carretera Cuautitlán–Teoloyucan, San Sebastián Xhala, Cuautitlán Izcalli, Estado de México, CP.54714, México

    Nayely Heinecke, Jorge Tórtora, Humberto A. Martínez, Víctor D. González-Fernández & Hugo Ramírez

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  1. Nayely Heinecke
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  2. Jorge Tórtora
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  3. Humberto A. Martínez
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  4. Víctor D. González-Fernández
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  5. Hugo Ramírez
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Corresponding author

Correspondence to Hugo Ramírez.

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Funding

This study was funded by the CONACyT project 221285 “Genotyping the env gene of retroviruses that impact the health of domestic ruminants”. We thank Jesús Zavaleta (GILSA), Javier Hernández, Miriam Rogel, the cattlemen who kindly provided the samples, and the staff of the laboratory of virology, genetics, and molecular biology of FES Cuautitlán. Nayely Heinecke thanks DGOAE-UNAM for the scholarship.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Conflict of interest

The authors have no financial or personal interests that could influence or bias the content of this article. The authors declare that they have no competing interests. All authors have seen and approved the manuscript.

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Heinecke, N., Tórtora, J., Martínez, H.A. et al. Detection and genotyping of bovine leukemia virus in Mexican cattle. Arch Virol 162, 3191–3196 (2017). https://doi.org/10.1007/s00705-017-3477-z

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  • DOI: https://doi.org/10.1007/s00705-017-3477-z

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