Skip to main content
SpringerLink
Log in

Densoviruses in oyster Crassostrea ariakensis

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Densoviruses have short ssDNA genomes and mainly infect arthropods. To characterize viral nucleic acid in shellfish, oysters (Crassostrea ariakensis) were analyzed using viral metagenomics. Two large de novo assembled contigs, CaaDV1 and CaaDV2, consisting of nearly complete densovirus genomes (5860 nucleotides (nt) and 4034 nt) with two major ambisense protein coding regions were identified. Several potential non-structural proteins and capsid proteins were encoded by these genomes, but these were divergent from the existing densoviral species. The NS1 protein of the two CaaDVs shared 43.3%~61.5% amino acid identities with the sea star-associated densovirus and cherax quadricarinatus densovirus, with the four species clustering by phylogenetic analysis. This is the first report of densovirus detection in shellfish, increasing the potential host range of densoviruses and the genetic diversity of the genus Ambidensovirus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Bergoin M, Tijssen P (2000) Molecular biology of Densovirinae. Parvoviruses. Karger Publishers, pp 12–32

  2. Bochow S, Condon K, Elliman J, Owens L (2015) First complete genome of an Ambidensovirus; Cherax quadricarinatus densovirus, from freshwater crayfish Cherax quadricarinatus. Mar Genomics 24:305–312

    Article  PubMed  Google Scholar 

  3. Brown J, Fauquet C, Briddon R, Zerbini M, Moriones E, Navas-Castillo J, King A, Adams M, Carstens E, Lefkowitz E (2012) In: King AMQ, Lefkowitz E, Adams MJ, Carstens, EB (eds) Virus taxonomy—ninth report of the International Committee on Taxonomy of Viruses, pp 351–373

  4. Campos CJ, Lees DN (2014) Environmental transmission of human noroviruses in shellfish waters. Appl Environ Microbiol 80:3552–3561

    Article  PubMed  PubMed Central  Google Scholar 

  5. Cotmore SF, Agbandje-McKenna M, Chiorini JA, Mukha DV, Pintel DJ, Qiu J, Soderlund-Venermo M, Tattersall P, Tijssen P, Gatherer D (2014) The family parvoviridae. Arch Virol 159:1239–1247

    Article  CAS  PubMed  Google Scholar 

  6. Deng X, Naccache SN, Ng T, Federman S, Li L, Chiu CY, Delwart EL (2015) An ensemble strategy that significantly improves de novo assembly of microbial genomes from metagenomic next-generation sequencing data. Nucleic Acids Res 43:e46–e46

    Article  PubMed  PubMed Central  Google Scholar 

  7. Hewson I, Button JB, Gudenkauf BM, Miner B, Newton AL, Gaydos JK, Wynne J, Groves CL, Hendler G, Murray M (2014) Densovirus associated with sea-star wasting disease and mass mortality. Proc Natl Acad Sci 111:17278–17283

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Kaufmann B, Bowman VD, Li Y, Szelei J, Waddell PJ, Tijssen P, Rossmann MG (2010) Structure of Penaeus stylirostris densovirus, a shrimp pathogen. J Virol 84:11289–11296

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Kittigul L, Singhaboot Y, Chavalitshewinkoon-Petmitr P, Pombubpa K, Hirunpetcharat C (2015) A comparison of virus concentration methods for molecular detection and characterization of rotavirus in bivalve shellfish species. Food Microbiol 46:161–167

    Article  CAS  PubMed  Google Scholar 

  11. Maalouf H, Pommepuy M, Le Guyader FS (2010) Environmental conditions leading to shellfish contamination and related outbreaks. Food Environ Virol 2:136–145

    Article  Google Scholar 

  12. Marchler-Bauer A, Derbyshire MK, Gonzales NR, Lu S, Chitsaz F, Geer LY, Geer RC, He J, Gwadz M, Hurwitz DI (2014) CDD: NCBI’s conserved domain database. Nucleic Acids Res:gku1221

  13. Tijssen P, Li Y, El-Far M, Szelei J, Letarte M, Zádori Z (2003) Organization and expression strategy of the ambisense genome of densonucleosis virus of Galleria mellonella. J Virol 77:10357–10365

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Wang H, Guo X (2009) Identification of Crassostrea ariakensis and related oysters by multiplex species-specific PCR. J Shellfish Res 27:481–487

    Article  Google Scholar 

  15. Yang S, Wang Y, Li W, Fan Z, Jiang L, Lin Y, Fu X, Shen Q, Sun Z, Wang X (2016) A novel bocavirus from domestic mink, China. Virus Genes 52:887–890

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the scientific fund from Health and family planning commission of Wuxi (No. Q201627), a grant from Public Health Research Center at Jiangnan University (No. JUPH201505), Fundamental Research Funds for the Central Universities (No. JUSRP11568), and a grant from Jiangsu Science and Technology Department (No. BM2015024-3).

Author information

Authors and Affiliations

  1. Wuxi School of Medicine, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People’s Republic of China

    Yan-Jun Kang, Wei Huang, A-li Zhao, Dan-Dan Lai & Yan-Qin Shen

  2. Public Health Research Center, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People’s Republic of China

    Yan-Jun Kang & Yan-Qin Shen

  3. Affiliated Hospital of Jiangnan University, Huihe Road 200, Wuxi, 214062, Jiangsu, People’s Republic of China

    Lin Shao

  4. Department of Laboratory Medicine, Blood Systems Research Institute, University of California San Francisco, San Francisco, CA, USA

    Xutao Deng & Eric Delwart

  5. School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 202103, Jiangsu, People’s Republic of China

    Wen Zhang

Authors
  1. Yan-Jun Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A-li Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dan-Dan Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lin Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yan-Qin Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xutao Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Eric Delwart
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Wen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

YJK, YQS and WZ conceived the study. YJK, WH, ALZ, DDL, and LS participated in the sampling and experiments. YJK, YQS, WZ, XTD and ED wrote the paper. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Wen Zhang.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kang, YJ., Huang, W., Zhao, Al. et al. Densoviruses in oyster Crassostrea ariakensis . Arch Virol 162, 2153–2157 (2017). https://doi.org/10.1007/s00705-017-3343-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-017-3343-z

Keywords

Search

Navigation