Abstract
High throughput sequencing (HTS) is a very powerful tool for detecting and discovering novel viral-like sequences without prior knowledge of the sequence. Here we describe the complete genome of a new vitivirus-like sequence that was found in arracacha (Arracacia xanthorrhiza) plants using HTS technology. The complete genome sequence was validated by Sanger sequencing. The genomic organization of the new putative vitivirus resembles that of grapevine virus B (GVB) and grapevine virus D (GVD). The putative coat protein showed 41 to 49% identity with similar proteins of known vitiviruses, while the RNA-dependent RNA polymerase shared 52 to 55% identity with those encoded by grapevine vitiviruses. Based on the demarcation criteria for the genus Vitivirus, the virus described in this work, provisionally named as “Arracacha virus V”, represents a novel species in this taxon.
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Acknowledgements
This work was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and EMBRAPA- Hortaliças. We thank Dr. Nuno Rodrigo Madeira for providing the infected arracacha samples. AKIN and TN are CNPq fellows. A.F.O. was recipient of a fellowship from CNPq-CsF Program.
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Oliveira, L.M., Orílio, A.F., Inoue-Nagata, A.K. et al. A novel vitivirus-like sequence found in Arracacia xanthorrhiza plants by high throughput sequencing. Arch Virol 162, 2141–2144 (2017). https://doi.org/10.1007/s00705-017-3326-0
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DOI: https://doi.org/10.1007/s00705-017-3326-0