Abstract
A double-stranded RNA (dsRNA) HBJZ1506 recovered from the phytopathogenic fungus Erysiphe cichoracearum infecting Calendula officinalis in Jingzhou, Hubei Province, China, was sequenced. HBJZ1506 comprises 11,908 nucleotides (nt) and contains a 11,859-nt-long open reading frame (ORF) coding for a polypeptide that is 61 % identical to that of a putative endornavirus named grapevine endophyte endornavirus (GeEV). The putative polyprotein has an RNA-dependent RNA polymerase (RdRp) domain and an RNA helicase domain, which show homology to and have an arrangement that is similar to that of their counterparts in approved or putative endornaviruses. In a phylogenetic tree constructed using amino acid sequences of the RdRp region of HBJZ1506 and selected endornaviruses, HBJZ1506 clustered with endornaviruses and formed a well-supported monophyletic branch with GeEV. These results suggest that HBJZ1506 might represent a novel endornavirus, for which the name Erysiphe cichoracearum endornavirus (EcEV) is proposed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abascal F, Zardoya R, Posada D (2005) ProtTest: selection of best-fit models of protein evolution. Bioinformatics 21:2104–2105
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Braun U, Cook TA, Inman AJ, Shin H-D (2002) The taxonomy of the powdery mildew fungi. In: Bélanger RR, Bushnell WR, Dik AJ, Carver TLW (eds) The powdery mildews. A comprehensive treatise. APS Press, St Paul, pp 13–55
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797
Espach Y, Maree HJ, Burger JT (2012) Complete genome of a novel endornavirus assembled from next-generation sequence data. J Virol 86:13142
Fukuhara T, Koga R, Aoki N, Yuki C, Yamamoto N, Oyama N, Udagawa T, Horiuchi H, Miyazaki S, Higashi Y, Takeshita M, Ikeda K, Arakawa M, Matsumoto N, Moriyama H (2006) The wide distribution of endornaviruses, large double-stranded RNA replicons with plasmid-like properties. Arch Virol 151:995–1002
Fukuhara T, Gibbs MJ (2012) Family Endornaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Tokyo, pp 519–521
Guo L, Yang X, Wu W, Tan G, Fang S, Zhang S, Li F (2016) Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus, family Totiviridae. Arch Virol 161:731–734
Lim S, Kim KH, Zhao F, Yoo RH, Igori D, Lee S-H, Moon JS (2015) Complete genome sequence of a novel endornavirus isolated from hot pepper. Arch Virol 160:3153–3156
Marchler-Bauer A, Lu S, Anderson JB et al (2011) CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res 39(suppl 1):D225–D229
Morris TJ, Dodds JA (1979) Isolation and analysis of double stranded RNA from virusinfected plant and fungal tissue. Phytopathology 69:854–858
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574
Ronquist F, Teslenko M, van der Mark P et al (2011) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542
Roossinck MJ, Sabanadzovic S, Okada R, Valverde RA (2011) The remarkable evolutionary history of endornaviruses. J Gen Virol 92:2674–2678
Roossinck MJ (2012) Persistent plant viruses: molecular hitchhikers or epigenetic elements [M]//Viruses: Essential Agents of Life. Springer, Netherlands, pp 177–186
Sabanadzovic S, Wintermantel WM, Valverde RA, McCreight JD, Aboughanem-Sabanadzovic N (2016) Cucumis melo endornavirus: genome organization, host range and co-divergence with the host. Virus Res 214:49–58
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Villanueva F, Sabanadzovic S, Valverde RA, Navas-Castillo J (2012) Complete genome sequence of a double-stranded RNA virus from avocado. J Virol 86:1282–1283
Acknowledgments
This work was supported by the National Natural Science Foundation of China (31301638, 31301642) and the Opening Fund of Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education (KF201501). We are grateful to Dr. Fangluan Gao for his help in constructing the BI tree.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The author declares no competing interests.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Z. Du and W. Lin contributed equally to this work.
Rights and permissions
About this article
Cite this article
Du, Z., Lin, W., Qiu, P. et al. Complete sequence of a double-stranded RNA from the phytopathogenic fungus Erysiphe cichoracearum that might represent a novel endornavirus. Arch Virol 161, 2343–2346 (2016). https://doi.org/10.1007/s00705-016-2911-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-016-2911-y