Abstract
H6 subtype avian influenza viruses (AIVs) possess the ability to cross the species barrier to infect mammals and pose a threat to human health. From June 2014 to July 2015, 12 H6N6 AIVs were isolated from chickens in live-poultry markets in Zhejiang Province, Eastern China. Phylogenetic analysis showed that these isolates received their genes from H6 and H9N2 subtype AIVs of poultry in China. These novel reassortant viruses showed moderate pathogenicity in mice and were able to replicate in mice without prior adaptation. Considering that novel reassorted H6N6 viruses were isolated from chickens in this study, it is possible that these chickens play an important role in the generation of novel reassorted H6N6 AIVs, and these results emphasize the need for continued surveillance of the H6N6 AIVs circulating in poultry.
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This study was funded by grants from the National Science Foundation of the People’s Republic of China (81502852), Zhejiang Provincial Natural Seience Foundation of China (Y15H190006), and the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases (Nos. 2015ZZ05 and 2016ZZ03).
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The animal experiment was approved by the First Affiliated Hospital, School of Medicine, Zhejiang University (No. 2015-15).
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Supplementary material 2 (DOC 624 kb) Fig. S1 Percentage of avian influenza virus positive poultry swabs by month. Cloacal swab samples were collected from live-poultry markets in Zheijang Province, Eastern China, from July 2014 to July 2015. The percentages of the swabs that tested positive for avian influenza virus each month during the collection period are shown
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Wu, H., Lu, R., Peng, X. et al. Isolation and genetic characterization of novel reassortant H6N6 subtype avian influenza viruses isolated from chickens in eastern China. Arch Virol 161, 1859–1872 (2016). https://doi.org/10.1007/s00705-016-2861-4
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DOI: https://doi.org/10.1007/s00705-016-2861-4