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Proteomic analysis of the plasma membrane-movement tubule complex of cowpea mosaic virus

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Abstract

Cowpea mosaic virus forms tubules constructed from the movement protein (MP) in plasmodesmata (PD) to achieve cell-to-cell movement of its virions. Similar tubules, delineated by the plasma membrane (PM), are formed protruding from the surface of infected protoplasts. These PM-tubule complexes were isolated from protoplasts by immunoprecipitation and analysed for their protein content by tandem mass spectrometry to identify host proteins with affinity for the movement tubule. Seven host proteins were abundantly present in the PM-tubule complex, including molecular chaperonins and an AAA protein. Members of both protein families have been implicated in establishment of systemic infection. The potential role of these proteins in tubule-guided cell-cell transport is discussed.

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Acknowledgements

Electron microscopy was done at the Wageningen Electron Microscopy Center (WEMC). This research was supported by the Earth and Life Sciences Division (ALW) of NOW (grant 817.02.016).

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    Authors and Affiliations

    1. Laboratory of Virology, Plant Sciences Group, Wageningen University, Wageningen, The Netherlands

      Paulus W. den Hollander, Hanke Bloksma & Jan W. M. van Lent

    2. Laboratory of Molecular Plant Virology, Department of Phytopathology, Federal University of Lavras, Lavras, Brazil

      Priscilla de Sousa Geraldino Duarte

    3. Laboratory of Biochemistry, Agrotechnology and Food Sciences, Wageningen University, Wageningen, The Netherlands

      Sjef Boeren

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    1. Paulus W. den Hollander
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    2. Priscilla de Sousa Geraldino Duarte
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    5. Jan W. M. van Lent
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    Corresponding author

    Correspondence to Jan W. M. van Lent.

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    P. W. den Hollander and P. de S. G. Duarte contributed equally

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    den Hollander, P.W., de Sousa Geraldino Duarte, P., Bloksma, H. et al. Proteomic analysis of the plasma membrane-movement tubule complex of cowpea mosaic virus. Arch Virol 161, 1309–1314 (2016). https://doi.org/10.1007/s00705-016-2757-3

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    • DOI: https://doi.org/10.1007/s00705-016-2757-3

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