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Complete nucleotide sequences of two new begomoviruses infecting the wild malvaceous plant Melochia sp. in Brazil

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Abstract

Wild malvaceous plants are hosts for a large number of begomoviruses (genus Begomovirus, family Geminiviridae) in both the Old World and the New World. Here, we report the complete genome sequences of two new begomoviruses from Melochia sp. plants from Brazil. The cloned bipartite genomes, composed of DNA-A and DNA-B, showed the typical organization of the New World begomoviruses but they were distantly related to the genomes of other begomoviruses. We propose the names Melochia mosaic virus and Melochia yellow mosaic virus for these begomoviruses.

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References

  1. Barreto SS, Hallwass M, Aquino OM, Inoue-Nagata AK (2013) A study of weeds as potential inoculum sources for a tomato-infecting begomovirus in central Brazil. Phytopathology 103:436–444

    Article  CAS  PubMed  Google Scholar 

  2. Briddon RW, Patil BL, Bagewadi B, Nawaz-ul-Rehman MS, Fauquet CM (2010) Distinct evolutionary histories of the DNA-A and DNA-B components of bipartite begomoviruses. BMC Evol Biol 10:97

    Article  PubMed Central  PubMed  Google Scholar 

  3. Brown JK, Fauquet CM, Briddon RW, Zerbini M, Moriones E, Navas-Castillo J (2012) Geminiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Ninth report of the ICTV. Elsevier/Academic Press, London, pp 351–373

    Google Scholar 

  4. Brown JK, Idris AM, Ostrow KM, Goldberg N, French R, Stenger DC (2005) Genetic and phenotypic variation of the Pepper golden mosaic virus complex. Phytopathology 95:1217–1224

    Article  CAS  PubMed  Google Scholar 

  5. Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF, Fiallo-Olivé E, Briddon RW, Hernández-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619

    Article  CAS  PubMed  Google Scholar 

  6. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small amounts of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  7. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Faria JC, Bezerra IC, Zerbini FM, Ribeiro SG, Lima MF (2000) Current status of geminivirus diseases in Brazil. Fitopatol Bras 25:125–137

    Google Scholar 

  9. Fernandes FR, Albuquerque LC, de Oliveira C, Cruz ARR, da Rocha WB, Pereira TG, Naito FYB, Dias NM, Nagata T, Faria JC, Zerbini FM, Aragão FJL, Inoue-Nagata AK (2011) Molecular and biological characterization of a new Brazilian begomovirus, euphorbia yellow mosaic virus (EuYMV), infecting Euphorbia heterophylla plants. Arch Virol 156:2063–2069

    Article  CAS  PubMed  Google Scholar 

  10. Fiallo-Olivé E, Navas-Castillo J, Moriones E, Martínez-Zubiaur Y (2010) Two novel begomoviruses belonging to different lineages infecting Rhynchosia minima. Arch Virol 155:2053–2058

    Article  PubMed  Google Scholar 

  11. Fourment M, Gibbs AJ, Gibbs MJ (2008) SWeBLAST: a sliding window web-based BLAST tool for recombinant analysis. J Virol Methods 152:98–101

    Article  CAS  PubMed  Google Scholar 

  12. Hollingsworth PM, Forrest LL, Spouge JL et al (2009) A DNA barcode for land plants. Proc Natl Acad Sci USA 106:12794–12797

    Article  PubMed Central  CAS  Google Scholar 

  13. Lima ATM, Sobrinho RR, González-Aguilera J, Rocha CS, Silva SJC, Xavier CAD, Silva FN, Duffy S, Zerbini FM (2013) Synonymous site variation due to recombination explains higher genetic variability in begomovirus populations infecting non-cultivated hosts. J Gen Virol 94:418–431

    Article  CAS  PubMed  Google Scholar 

  14. Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Muhire B, Varsani A, Martin DP (2014) SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:e108277

    Article  PubMed Central  PubMed  Google Scholar 

  16. Navas-Castillo J, Fiallo-Olivé E, Sánchez-Campos S (2011) Emerging virus diseases transmitted by whiteflies. Annu Rev Phytopatol 49:219–248

    Article  CAS  Google Scholar 

  17. Rocha CS, Castillo-Urquiza GP, Lima ATM, Silva FN, Xavier CAD, Hora-Junior BT, Beserra-Junior JEA, Malta AWO, Martin DP, Varsani A, Alfenas-Zerbini P, Mizubuti ESG, Zerbini FM (2013) Brazilian begomovirus populations are highly recombinant, rapidly evolving, and segregated based on geographical location. J Virol 87:5784–5799

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Silva SJC, Castillo-Urquiza GP, Hora-Júnior BT, Assunçao IP, Lima GSA, Pio-Ribeiro G, Mizubuti ESG, Zerbini FM (2012) Species diversity, phylogeny and genetic variability of begomovirus populations infecting leguminous weeds in northeastern Brazil. Plant Pathol 61:457–467

    Article  Google Scholar 

  19. Silva SJC, Castillo-Urquiza GP, Hora-Júnior BT, Assunção IP, Lima GSA, Pio-Ribeiro G, Mizubuti ESG, Zerbini FM (2011) High genetic variability and recombination in a begomovirus population infecting the ubiquitous weed Cleome affinis in north-eastern Brazil. Arch Virol 156:2205–2213

    Article  PubMed  Google Scholar 

  20. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Varsani A, Navas-Castillo J, Moriones E, Hernández-Zepeda C, Idris A, Brown JK, Zerbini FM (2014) Establishment of three new genera in the family Geminiviridae: Becurtovirus, Eragrovirus and Turncurtovirus. Arch Virol 159:2193–2203

    Article  CAS  PubMed  Google Scholar 

  22. Wyant PS, Strohmeier S, Schafer B, Krenz B, Assunçao IP, Lima GSD, Jeske H (2012) Circular DNA genomics (circomics) exemplified for geminiviruses in bean crops and weeds of northeastern Brazil. Virology 427:151–157

    Article  CAS  PubMed  Google Scholar 

  23. Zerbini FM, Andrade EC, Barros DR, Ferreira SS, Lima ATM, Alfenas PF, Mello RN (2005) Traditional and novel strategies for geminivirus management in Brazil. Australas Plant Path 34:475–480

    Article  Google Scholar 

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Acknowledgments

This investigation was supported by the program Ciência sem Fronteiras (Grant 401838/2013-7, CNPq, Brazil) and by CNPq grant 483607/2013-4 to FMZ. EFO was the recipient of a Visiting Researcher Fellowship (CAG-BPV-00060-13) from Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil. JNC was the recipient of a Special Visiting Researcher Fellowship (313813/2013-2) from the program Ciência sem Fronteiras and is a member of the Research Group AGR-214, partially funded by Consejería de Economía, Innovación y Ciencia, Junta de Andalucía, Spain, cofinanced by FEDER-FSE.

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Authors and Affiliations

  1. Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”, 29750, Algarrobo-Costa, Málaga, Spain

    Elvira Fiallo-Olivé & Jesús Navas-Castillo

  2. Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Elvira Fiallo-Olivé, F. Murilo Zerbini & Jesús Navas-Castillo

  3. National Research Institute for Plant-Pest Interactions (INCT-IPP), Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Elvira Fiallo-Olivé, F. Murilo Zerbini & Jesús Navas-Castillo

Authors
  1. Elvira Fiallo-Olivé
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  2. F. Murilo Zerbini
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  3. Jesús Navas-Castillo
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Correspondence to Elvira Fiallo-Olivé.

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Fiallo-Olivé, E., Zerbini, F.M. & Navas-Castillo, J. Complete nucleotide sequences of two new begomoviruses infecting the wild malvaceous plant Melochia sp. in Brazil. Arch Virol 160, 3161–3164 (2015). https://doi.org/10.1007/s00705-015-2619-4

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  • DOI: https://doi.org/10.1007/s00705-015-2619-4

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