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Comparative molecular genetic analysis of simian and human HIV-1 integrase interactor INI1/SMARCB1/SNF5

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Abstract

Human integrase interactor 1 (INI1/SMARCB1/SNF5) is a chromatin-remodeling molecule that binds to HIV-1 integrase and enhances proviral DNA integration. INI1 is also known as a tumor suppressor gene and has been found to be mutated in several aggressive tumors such as rhabdoid and lymphoid tumors. To study the function of simian INI1, we screened and cloned simian INI1 cDNA from B lymphoma cells of rhesus monkeys using RT-PCR. Sequence analysis showed 23 single nucleotide differences compared to the human ortholog, which, however, did not result in amino acid changes, and the amino acid sequence is therefore 100 % conserved between human and simian INI1. Two alternatively spliced isoforms, INI1a and INI1b, were also found in simian INI1. These two isoforms did not show any functional difference in HIV-1 proviral DNA integration and nuclear localization, suggesting that the specificity of simian INI1 would not be a factor preventing HIV-1 infection of a simian host. Nevertheless, INI1b is expressed only in established cancer cell lines such as Jurkat and COS-7 cells, and not in primary cells, suggesting that INIlb could be an indicator of cell transformation.

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Acknowledgments

This work was supported by the David Geffen Research Fellowship for AIDS Research (D. P.) and NIH/NIDDK R01 DK099055 (I-W. P).

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Authors and Affiliations

  1. Department of Immunology and Microbiology, University of Colorado School of Medicine, 12800 E. 19th Ave. MS 8333, Aurora, CO, 80045, USA

    Dohun Pyeon

  2. Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA

    Dohun Pyeon

  3. Department of Cell Biology and Anatomy, Graduate School of Biomedical Sciences, University of North Texas Health Science Center, CBH-316, 3500 Camp Bowie Blvd, Fort Worth, TX, 76107, USA

    Lenore Price & In-Woo Park

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  1. Dohun Pyeon
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  2. Lenore Price
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  3. In-Woo Park
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Correspondence to Dohun Pyeon or In-Woo Park.

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Pyeon, D., Price, L. & Park, IW. Comparative molecular genetic analysis of simian and human HIV-1 integrase interactor INI1/SMARCB1/SNF5. Arch Virol 160, 3085–3091 (2015). https://doi.org/10.1007/s00705-015-2585-x

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  • DOI: https://doi.org/10.1007/s00705-015-2585-x

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