Skip to main content
SpringerLink
Log in

Bufavirus in fecal specimens of patients with and without diarrhea in Thailand

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Bufavirus (BuV) was initially discovered in fecal samples from children with acute diarrhea. In this study, we determined the prevalence, distribution, and genotype(s) of BuV in Thailand. A total of 1,495 diarrheal and 741 non-diarrheal stool specimens were collected and analyzed. A portion of the NS1 gene of BuV was amplified by nested RT-PCR. Phylogenetic analysis was performed to classify the BuV strains found. We detected bufavirus (BuV) in diarrheal (4/1495; 0.27 %) but not in non-diarrheal specimens (0/726). All four strains belonged to BuV genotype 1. BuV could be detected in adults and children, but its role in causing acute diarrhea remains unclear.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Phan TG, Vo NP, Bonkoungou IJ, Kapoor A, Barro N, O’Ryan M, Kapusinszky B, Wang C, Delwart E (2012) Acute diarrhea in West African children: diverse enteric viruses and a novel parvovirus genus. J Virol 86:11024–11030

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Cotmore SF, Agbandje-McKenna M, Chiorini JA, Mukha DV, Pintel DJ, Qiu J et al (2014) The family Parvoviridae. Arch Virol 159:1239–1247

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  3. Yahiro T, Wangchuk S, Tshering K, Bandhari P, Zangmo S, Dorji T et al (2014) Novel human bufavirus genotype 3 in children with severe diarrhea, Bhutan. Emerg Infect Dis 20:1037–1039

    Article  PubMed Central  PubMed  Google Scholar 

  4. Väisänen E, Kuisma I, Phan TG, Delwart E, Lappalainen M, Tarkka E et al (2014) Bufavirus in feces of patients with gastroenteritis, Finland. Emerg Infect Dis 20:1077–1080

    Article  PubMed Central  PubMed  Google Scholar 

  5. Smits SL, Schapendonk CM, van Beek J, Vennema H, Schürch AC, Schipper D, Bodewes R et al (2014) New viruses in idiopathichuman diarrhea cases, the Netherlands. Emerg Infect Dis 20:1218–1222

    Article  PubMed Central  PubMed  Google Scholar 

  6. Maiklang O, Vutithanachot V, Vutithanachot C, Hacharoen P, Chieochansin T, Poovorawan Y (2012) Prevalence of group A genotype human rotavirus among children with diarrhea in Thailand, 2009-2011. Southeast Asian J Trop Med Public Health 43:904–9016

    PubMed  Google Scholar 

  7. Puenpa J, Mauleekoonphairoj J, Linsuwanon P, Suwannakarn K, Chieochansin T, Korkong S, Theamboonlers A, Poovorawan Y (2014) Prevalence and characterization of enterovirus infections among pediatric patients with hand foot mouth disease, herpangina and influenza like illness in Thailand, 2012. PLoS One 9:e98888

    Article  PubMed Central  PubMed  Google Scholar 

  8. Hansman GS, Oka T, Katayama K, Takeda N (2007) Human sapoviruses: genetic diversity, recombination, and classification. Rev Med Virol 17:133–141

    Article  CAS  PubMed  Google Scholar 

  9. Harvala H, Robertson I, Leitch ECM, Benschop K, Wolthers KC, Templeton K, Simmonds P (2008) Epidemiology and clinical associations of human parechovirusrespiratory infections. J Clin Microbiol 46:3446–3453

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Chieochansin T, Vichiwattana P, Korkong S, Theamboonlers A, Poovorawan Y (2011) Molecular epidemiology, genome characterization, and recombination event of human parechovirus. Virology 421:159–166

    Article  CAS  PubMed  Google Scholar 

  11. Chieochansin T, Kapoor A, Delwart E, Poovorawan Y, Simmonds P (2009) Absence of detectable replication of the human bocavirus species 2 in the respiratory tract despite frequent detection in faecal samples. Emerg Infect Dis 15:1503–1505

    Article  PubMed Central  PubMed  Google Scholar 

  12. Sriwanna P, Chieochansin T, Vuthitanachot C, Vuthitanachot V, Theamboonlers A, Poovorawan Y (2013) Molecular characterization of human adenovirus infection in Thailand, 2009–2012. Virol J 10:193

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Sanchez-Villeda H, Schroeder S, Flint-Garcia S, Guill KE, Yamasaki M, McMullen MD (2008) DNAAlignEditor: DNA alignmenteditor tool. BMC Bioinformatics 9:154

    Article  PubMed Central  PubMed  Google Scholar 

  14. Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256

    Article  CAS  PubMed  Google Scholar 

  15. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by Ratchadaphisek Somphot Fund for postdoctoral fellowship; National Research Council of Thailand; The Higher Education Research Promotion and National Research University Project of Thailand Office of the Higher Education Commission (WCU-58-006-HR, WCU007-HR57, WCU 001-HR57); the Center of Excellence in Clinical Virology, Chulalongkorn University; Chulalongkorn University Centenary Academic Development Project (CU56-HR01); the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University (RES560530093); the Outstanding Professor of the Thailand Research Fund (DPG5480002); the Research Chair Grant from the National Science and Technology Development Agency; MK restaurant Company Limited; The Siam Cement Group PcL; and King Chulalongkorn Memorial Hospital. We would like to thank Dr. Sompong Vongpunsawad for reviewing the manuscript.

Conflict of interest

The authors have no conflicts of interest to declare.

Author information

Authors and Affiliations

  1. Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330, Thailand

    Thaweesak Chieochansin, Apiradee Theamboonlers & Yong Poovorawan

  2. Chumphae Hospital, Chum Phae, Khon Kaen, 40130, Thailand

    Viboonsak Vutithanachot

  3. Division of Molecular Medicine, Department of Research and Development, Faculty of Medicine, Mahidol University, Bangkok, 10700, Thailand

    Thaweesak Chieochansin

Authors
  1. Thaweesak Chieochansin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Viboonsak Vutithanachot
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Apiradee Theamboonlers
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yong Poovorawan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yong Poovorawan.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chieochansin, T., Vutithanachot, V., Theamboonlers, A. et al. Bufavirus in fecal specimens of patients with and without diarrhea in Thailand. Arch Virol 160, 1781–1784 (2015). https://doi.org/10.1007/s00705-015-2441-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-015-2441-z

Keywords

Search

Navigation