Abstract
During surveillance for avian influenza viruses (AIVs) in live-poultry markets (LPMs) in eastern China in 2013, one H5N2 AIV was isolated from a duck. Phylogenetic analysis showed that the hemagglutinin of this strain belongs to clade 2.3.4 and received its genes from H5, H3 and H6 AIVs of poultry in China. The virulence of this strain was examined in chickens and mice, and it was found to be highly pathogenic in chickens but demonstrated moderate pathogenicity in mice. These results suggest that active surveillance of AIVs in LPMs should be used in an early warning system for avian influenza outbreaks.
References
Zhu X, Yu W, McBride R, Li Y, Chen LM, Donis RO, Tong S, Paulson JC, Wilson IA (2013) Hemagglutinin homologue from H17N10 bat influenza virus exhibits divergent receptor-binding and pH-dependent fusion activities. Proc Natl Acad Sci USA 110:1458–1463
Tong S, Zhu X, Li Y, Shi M, Zhang J, Bourgeois M, Yang H, Chen X, Recuenco S, Gomez J, Chen LM, Johnson A, Tao Y, Dreyfus C, Yu W, McBride R, Carney PJ, Gilbert AT, Chang J, Guo Z, Davis CT, Paulson JC, Stevens J, Rupprecht CE, Holmes EC, Wilson IA, Donis RO (2013) New world bats harbor diverse influenza A viruses. PLoS Pathog 9:e1003657
Kawaoka Y, Chambers TM, Sladen WL, Webster RG (1988) Is the gene pool of influenza viruses in shorebirds and gulls different from that in wild ducks? Virology 163:247–250
Liu M, He S, Walker D, Zhou N, Perez DR, Mo B, Li F, Huang X, Webster RG, Webby RJ (2003) The influenza virus gene pool in a poultry market in South central China. Virology 305:267–275
Cardona C, Yee K, Carpenter T (2009) Are live bird markets reservoirs of avian influenza? Poult Sci 88:856–859
Li KS, Guan Y, Wang J, Smith GJ, Xu KM, Duan L, Rahardjo AP, Puthavathana P, Buranathai C, Nguyen TD, Estoepangestie AT, Chaisingh A, Auewarakul P, Long HT, Hanh NT, Webby RJ, Poon LL, Chen H, Shortridge KF, Yuen KY, Webster RG, Peiris JS (2004) Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia. Nature 430:209–213
Li Y, Shi J, Zhong G, Deng G, Tian G, Ge J, Zeng X, Song J, Zhao D, Liu L, Jiang Y, Guan Y, Bu Z, Chen H (2010) Continued evolution of H5N1 influenza viruses in wild birds, domestic poultry, and humans in China from 2004 to 2009. J Virol 84:8389–8397
Smith GJ, Vijaykrishna D, Ellis TM, Dyrting KC, Leung YH, Bahl J, Wong CW, Kai H, Chow MK, Duan L, Chan AS, Zhang LJ, Chen H, Luk GS, Peiris JS, Guan Y (2009) Characterization of avian influenza viruses A (H5N1) from wild birds, Hong Kong, 2004-2008. Emerg Infect Dis 15:402–407
Cheng MC, Soda K, Lee MS, Lee SH, Sakoda Y, Kida H, Wang CH (2010) Isolation and characterization of potentially pathogenic H5N2 influenza virus from a chicken in Taiwan in 2008. Avian Dis 54:885–893
Lee JH, Pascua PN, Song MS, Baek YH, Kim CJ, Choi HW, Sung MH, Webby RJ, Webster RG, Poo H, Choi YK (2009) Isolation and genetic characterization of H5N2 influenza viruses from pigs in Korea. J Virol 83:4205–4215
Wu HS, Yang JR, Liu MT, Yang CH, Cheng MC, Chang FY (2014) Influenza A(H5N2) virus antibodies in humans after contact with infected poultry, Taiwan, 2012. Emerg Infect Dis 20:857–860
Ogata T, Yamazaki Y, Okabe N, Nakamura Y, Tashiro M, Nagata N, Itamura S, Yasui Y, Nakashima K, Doi M, Izumi Y, Fujieda T, Yamato S, Kawada Y (2008) Human H5N2 avian influenza infection in Japan and the factors associated with high H5N2-neutralizing antibody titer. J Epidemiol 18:160–166
Shafir SC, Fuller T, Smith TB, Rimoin AW (2012) A national study of individuals who handle migratory birds for evidence of avian and swine-origin influenza virus infections. J Clin Virol 54:364–367
Wu HB, Guo CT, Lu RF, Xu LH, Wo EK, You JB, Wang YT, Wang QG, Wu NP (2012) Genetic characterization of subtype H1 avian influenza viruses isolated from live poultry markets in Zhejiang Province, China, in 2011. Virus Genes 44:441–449
Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR (2001) Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol 146:2275–2289
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Hu X, Liu D, Wang M, Yang L, Zhu Q, Li L, Gao GF (2011) Clade 2.3.2 avian influenza virus (H5N1), Qinghai Lake region, China, 2009-2010. Emerg Infect Dis 17:560–562
Hai-bo W, Chao-tan G, Ru-feng L, Li-hua X, En-kang W, Jin-biao Y, Yi-ting W, Qiao-gang W, Nan-ping W (2012) Characterization of a highly pathogenic H5N1 avian influenza virus isolated from ducks in Eastern China in 2011. Arch Virol 157:1131–1136
Smith GJ, Fan XH, Wang J, Li KS, Qin K, Zhang JX, Vijaykrishna D, Cheung CL, Huang K, Rayner JM, Peiris JS, Chen H, Webster RG, Guan Y (2006) Emergence and predominance of an H5N1 influenza variant in China. Proc Natl Acad Sci USA 103:16936–16941
Zhao G, Gu X, Lu X, Pan J, Duan Z, Zhao K, Gu M, Liu Q, He L, Chen J, Ge S, Wang Y, Chen S, Wang X, Peng D, Wan H, Liu X (2012) Novel reassortant highly pathogenic H5N2 avian influenza viruses in poultry in China. PLoS One 7:e46183
Zhao Q, Li Q, Zhong L, Gu M, Zhu J, Zhao G, Chen C, Wang X, Liu X (2013) Complete genomic sequence of a novel reassortant H4N2 avian influenza virus isolated from domestic ducks in Jiangsu, China. Genome Announc 1:e0009113
Huang K, Zhu H, Fan X, Wang J, Cheung CL, Duan L, Hong W, Liu Y, Li L, Smith DK, Chen H, Webster RG, Webby RJ, Peiris M, Guan Y (2012) Establishment and lineage replacement of H6 influenza viruses in domestic ducks in southern China. J Virol 86:6075–6083
Zhang Y, Zhu J, Li Y, Bradley KC, Cao J, Chen H, Jin M, Zhou H (2013) Glycosylation on hemagglutinin affects the virulence and pathogenicity of pandemic H1N1/2009 influenza A virus in mice. PLoS One 8:e61397
Helenius A, Aebi M (2004) Roles of N-linked glycans in the endoplasmic reticulum. Annu Rev Biochem 73:1019–1049
Shinya K, Hamm S, Hatta M, Ito H, Ito T, Kawaoka Y (2004) PB2 amino acid at position 627 affects replicative efficiency, but not cell tropism, of Hong Kong H5N1 influenza A viruses in mice. Virology 320:258–266
Zhu Q, Yang H, Chen W, Cao W, Zhong G, Jiao P, Deng G, Yu K, Yang C, Bu Z, Kawaoka Y, Chen H (2008) A naturally occurring deletion in its NS gene contributes to the attenuation of an H5N1 swine influenza virus in chickens. J Virol 82:220–228
Jiao P, Tian G, Li Y, Deng G, Jiang Y, Liu C, Liu W, Bu Z, Kawaoka Y, Chen H (2008) A single-amino-acid substitution in the NS1 protein changes the pathogenicity of H5N1 avian influenza viruses in mice. J Virol 82:1146–1154
Pearson J E (Office International des Epizooties) (2003) International standards for the control of avian influenza. Avian Dis 47(3 Suppl):972–975
Chen H (2009) Avian influenza vaccination: the experience in China. Rev Sci Tech 28:267–274
Zhao K, Gu M, Zhong L, Duan Z, Zhang Y, Zhu Y, Zhao G, Zhao M, Chen Z, Hu S, Liu W, Liu X, Peng D (2013) Characterization of three H5N5 and one H5N8 highly pathogenic avian influenza viruses in China. Vet Microbiol 163:351–357
Wang M, Di B, Zhou DH, Zheng BJ, Jing H, Lin YP, Liu YF, Wu XW, Qin PZ, Wang YL, Jian LY, Li XZ, Xu JX, Lu EJ, Li TG, Xu J (2006) Food markets with live birds as source of avian influenza. Emerg Infect Dis 12:1773–1775
Reed LJ, Muench H (1938) A simple method for estimating fifty percent endpoints. Am J Hyg 27:493–497
Acknowledgements
This study was supported by grants from the National Key Technologies R&D Programme for the 12th Five-Year Plan of China (2012ZX1000-004-005) and the State Key Laboratory of Independent Task (No. 2014ZZ12).
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Wu, H., Peng, X., Xu, L. et al. Characterization of a novel highly pathogenic H5N2 avian influenza virus isolated from a duck in eastern China. Arch Virol 159, 3377–3383 (2014). https://doi.org/10.1007/s00705-014-2193-1
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DOI: https://doi.org/10.1007/s00705-014-2193-1