Abstract
Amoebas of the genus Acanthamoeba are protists that are associated with human disease and represent a public health concern. They can harbor pathogenic microorganisms, acting as a platform for pathogen replication. Acanthamoeba polyphaga mimivirus (APMV), the type species of the genus Mimivirus, family Mimiviridae, represents the largest group of amoeba-associated viruses that has been described to date. Recent studies have demonstrated that APMV and other giant viruses may cause pneumonia. Amoebas can survive in most environments and tolerate various adverse conditions, including UV light irradiation, high concentrations of disinfectants, and a broad range of temperatures. However, it is unknown how the amoebal intracellular environment influences APMV stability and resistance to adverse conditions. Therefore, in this work, we evaluated the stability of APMV, either purified or carried by the amoeba host, under extreme conditions, including UV irradiation, heat and exposure to six different chemical biocides. After each treatment, the virus was titrated in amoebas using the TCID50 method. APMV was more stable in all resistance tests performed when located inside its host. Our results demonstrate that Acanthamoeba acts as a natural bunker for APMV, increasing viral resistance to extreme physical and chemical conditions. The data raise new questions regarding the survival of APMV in nature and in hospital environments.
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Acknowledgments
We thank João Rodrigues dos Santos, Gisele Cirilo dos Santos and colleagues from Gepvig and the Laboratório de Vírus for their excellent technical support. We also thank Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG) and the Pró-Reitoria de Pesquisa da Universidade Federal de Minas Gerais. Financial support was provided by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Ministério da Agricultura, Pecuária e Abastecimento (MAPA) and Pro-Reitoria de Pesquisa da Universidade Federal de Minas Gerais (PRPq-UFMG). E.G. Kroon is a researcher from CNPq.
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Boratto, P.V.M., Dornas, F.P., Andrade, K.R. et al. Amoebas as mimivirus bunkers: increased resistance to UV light, heat and chemical biocides when viruses are carried by amoeba hosts. Arch Virol 159, 1039–1043 (2014). https://doi.org/10.1007/s00705-013-1924-z
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DOI: https://doi.org/10.1007/s00705-013-1924-z