Abstract
A new virus was isolated from a lettuce plant grown in an open field in the Netherlands in 2011. This plant was showing conspicuous symptoms that consisted of necrosis and moderate leaf curling. The virus was mechanically transferred to indicator plants, and a total RNA extract of one of these indicator plants was used for next-generation sequencing. Analysis of the sequences that were obtained and further biological studies showed that the virus was related to, but clearly distinct from, viruses belonging to the genus Torradovirus. The name “lettuce necrotic leaf curl virus” (LNLCV) is proposed for this new torradovirus.
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References
Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Argos P (1988) A sequence motif in many polymerases. Nucleic Acids Res 16:9909–9916
Batuman O, Kuo YW, Palmieri M, Rojas MR, Gilbertson RL (2010) Tomato chocolate spot virus, a member of a new torradovirus species that causes a necrosis-associated disease of tomato in Guatemala. Arch Virol 155:857–869
Bazan JF, Fletterick RJ (1988) Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: Structural and functional implications. Proc Natl Acad Sci USA 85:7872–7876
Le Gall O, Sanfaçon H, Ikegami M, Iwanami T, Jones T, Karasev A, Lehto K, Wellink J, Wetzel T, Yoshikawa N (2007) Cheravirus and Sadwavirus: two unassigned genera of plant positive-sense single-stranded RNA viruses formerly considered atypical members of the genus Nepovirus (family Comoviridae). Arch Virol 152:1767–1774
Le Gall O, Christian P, Fauquet CM, King AMQ, Knowles NJ, Nakashima N, Stanway G, Gorbalenya AE (2008) Picornavirales, a proposed order of positive-sense single-stranded RNA viruses with a pseudo-T = 3 virion architecture. Arch Virol 153:715–727
Sanfaçon H, Iwanami T, Karasev AV, Van der Vlugt RAA, Wellink J, Wetzel T, Yoshikawa N (2011) Secoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Ninth report of the international committee on taxonomy of viruses. Elsevier Academic Press, San Diego, pp 881–899
Verbeek M, Dullemans AM, Van den Heuvel JFJM, Maris PC, Van der Vlugt RAA (2007) Identification and characterisation of tomato torrado virus, a new plant picorna-like virus from tomato. Arch Virol 152:881–890
Verbeek M, Dullemans AM, Van den Heuvel JFJM, Maris PC, Van der Vlugt RAA (2008) Tomato marchitez virus, a new plant picorna-like virus from tomato related to tomato torrado virus. Arch Virol 153:127–134
Verbeek M, Dullemans AM, Van den Heuvel JFJM, Maris PC, Van der Vlugt RAA (2010) Tomato chocolàte virus: a new plant virus infecting tomato and a proposed member of the genus Torradovirus. Arch Virol 155:751–755
Verbeek M, Tang J, Ward LI (2012) Two generic PCR primer sets for the detection of members of the genus Torradovirus. J Virol Methods 185:184–188
Zhang Z, Schwartz S, Wagner L, Miller W (2000) A Greedy algorithm for aligning DNA sequences. J Comput Biol 7:203–214
Acknowledgements
The authors are grateful to E. Schijlen and W. te Lintel Hekkert for their help with next-generation sequencing and data analysis.
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M. Verbeek and A. M. Dullemans contributed equally to this research.
Nucleotide sequence data reported are available in the GenBank database under accession numbers KC855266 (RNA1) and KC855267 (RNA2).
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Verbeek, M., Dullemans, A.M., van Raaij, H.M.G. et al. Lettuce necrotic leaf curl virus, a new plant virus infecting lettuce and a proposed member of the genus Torradovirus . Arch Virol 159, 801–805 (2014). https://doi.org/10.1007/s00705-013-1835-z
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DOI: https://doi.org/10.1007/s00705-013-1835-z