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Transcriptional profile of Marek’s disease virus genes in chicken thymus during different phases of MDV infection

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Abstract

In this study, 86 Marek’s disease virus (MDV) transcripts were detected in chicken thymus infected with RB1B strain. Forty-seven of them, which were mainly involved in viral replication and immune escape, were detected at 7 days postinfection (dpi). Expression of most of the genes was increased at 21 and 28 dpi but reduced or shut down at 14 dpi. Unlike others tissues, we found that a latent infection was established at 14 dpi in infected thymus. Here, we show the kinetics of expression of MDV transcripts and their relative expression in infected thymus.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (30871873, 31072135), Major Basic Research of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Grant No. 12KJA23001) and The Program for Changjiang Scholars and Innovative Research Team in University (IRT0978).

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Authors and Affiliations

  1. Ministry of Education Key Lab for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu, 225009, People’s Republic of China

    Xuming Hu, Aijian Qin, Kun Qian & Hongxia Shao

  2. Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, People’s Republic of China

    Aijian Qin, Ji Miao, Wencai Xu, Chuan Yu, Kun Qian & Hongxia Shao

Authors
  1. Xuming Hu
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  2. Aijian Qin
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  3. Ji Miao
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  4. Wencai Xu
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  5. Chuan Yu
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  6. Kun Qian
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  7. Hongxia Shao
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Corresponding author

Correspondence to Aijian Qin.

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Primers used for real-time PCR (DOC 34 kb)

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Hu, X., Qin, A., Miao, J. et al. Transcriptional profile of Marek’s disease virus genes in chicken thymus during different phases of MDV infection. Arch Virol 158, 1787–1793 (2013). https://doi.org/10.1007/s00705-013-1665-z

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  • DOI: https://doi.org/10.1007/s00705-013-1665-z

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