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Molecular characterization of a new lytic bacteriophage isolated from cheese whey

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Abstract

In this study, we isolated and characterized a lytic Lactococcus lactis bacteriophage from the sera of a failed fermentation. The phage was isolated and cultured in L. lactis subsp. cremoris in M17 medium. The isolated bacteriophage was characterized by multiplex PCR, pulsed-field electrophoresis, DNA restriction digestion, analysis of the N-terminal sequence of the phage major structural protein, transmission electron microscopy and sequencing and analysis of a conserved fragment of its genome. Analysis of the viral genome indicates that its genome is composed of a DNA strand of approximately 48 kb in length, and PCR and microscopy confirmed that IL-P1 belongs to the group of 936-type phages in the family Siphoviridae, which is the most abundant type of lactococcal virus in dairy products worldwide. To our knowledge, this is the first report of a virus within this family that has a presumptive genome larger than 40 kb.

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Acknowledgments

We would like to thank the Nucleus of Microscopy and Microanalysis (NMM) and the Nucleus of Biomolecules (NuBio) of the Federal University of Viçosa, for providing materials and technical support. This study was supported by grants from the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and a Master fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) to MRE. The funders had no role in the study design, data collection, analysis, decision to publish, or preparation of this manuscript.

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Authors and Affiliations

  1. Laboratory of Molecular Immunovirology, Department of General Biology, Federal University of Viçosa, Viçosa, Brazil

    M. R. Eller, R. S. Dias, L. L. Oliveira, E. A. M. Silva, C. C. da Silva & S. O. De Paula

  2. Department of Microbiology, Federal University of Viçosa, Viçosa, Brazil

    M. R. Eller & C. A. De Moraes

  3. Department of Food Technology, Federal University of Viçosa, Av PH Rolfs, s/n, Viçosa, Minas Gerais, 36570-000, Brazil

    A. F. De Carvalho

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  1. M. R. Eller
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  2. R. S. Dias
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  3. C. A. De Moraes
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  4. A. F. De Carvalho
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  5. L. L. Oliveira
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  6. E. A. M. Silva
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  7. C. C. da Silva
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  8. S. O. De Paula
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Correspondence to S. O. De Paula.

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Eller, M.R., Dias, R.S., De Moraes, C.A. et al. Molecular characterization of a new lytic bacteriophage isolated from cheese whey. Arch Virol 157, 2265–2272 (2012). https://doi.org/10.1007/s00705-012-1432-6

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  • DOI: https://doi.org/10.1007/s00705-012-1432-6

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