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Molecular characterization of a rare G9P[23] porcine rotavirus isolate from China

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Abstract

The fifth most important G genotype, G9 rotavirus, is recognized as an emerging genotype that is spreading around the world. Sequence analysis was completed of a rare group A rotavirus, strain G9P[23], that was designated rotavirus A pig/China/NMTL/2008/G9P[23] and abbreviated as NMTL. It was isolated from a piglet with diarrhea in China. Nucleotide sequence analysis revealed that the VP7 gene clustered within the G9 lineage VId. The VP4 gene clustered within the rare P[23] genotype. NMTL is the first porcine G9 stain reported in China. Thus, to further characterize the evolutionary diversity of the NMTL strain, all gene segments were used to draw a phylogenetic tree. Based on the new classification system of rotaviruses, the NMTL sequence revealed a G9–P[23]–I5–R1–C1–M1–A8–N1–T1–E1–H1 genotype with close similarity to human Wa-like and porcine strains. The results showed that (i) NSP2 and NSP4 genes of NMTL exhibited higher genetic relatedness to human group A rotaviruses than to porcine strains, (ii) the VP2 and VP4 genes clustered with porcine and porcine-like human strains, and (iii) VP1 genes clustered apart from the Wa-like human and porcine clusters. In view of rotavirus evolution, this report provides additional evidence to support the notion that the human and porcine rotavirus genomes might be related.

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References

  1. Abe M, Ito N, Morikawa S, Takasu M, Murase T, Kawashima T, Kawai Y, Kohara J, Sugiyama M (2009) Molecular epidemiology of rotaviruses among healthy calves in Japan: isolation of a novel bovine rotavirus bearing new P and G genotypes. Virus Res 144:250–257

    Article  PubMed  CAS  Google Scholar 

  2. Clark HF, Hoshino Y, Bell LM, Groff J, Hess G, Bachman P, Offit PA (1987) Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol 25:1757–1762

    PubMed  CAS  Google Scholar 

  3. Li Dan-di, Liu Na, Jie-mei Yu, Zhang Qing, Cui Shu-xian, Zhang Dong-liang, Yang Su-Hua, Cao Da-jiang, Zi-qian Xu, Duan Zhao-jun (2009) Molecular epidemiology of G9 rotavirus strains in children with diarrhea hospitalized in Mainland China from January 2006 to December 2007. Vaccine 27S:F40–F45

    Article  Google Scholar 

  4. Dennehy PH (2008) Rotavirus vaccines: an overview. J Clin Microbiol 21:198–208

    Google Scholar 

  5. Estes MK, Kapikian A (2007) Rotaviruses. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE(eds) Fields Virology, 5th edn. Kluwer Health/Lippincott, Williams and Wilkins, Philadelphia, pp 1917–1974

  6. Fu C, Wang M, Liang J, He T, Wang D, Xu J et al (2007) Effectiveness of Lanzhou lamb rotavirus vaccine against rotavirus gastroenteritis requiring hospitalization: a matched case-control study. Vaccine 25(52):8756–8761

    Article  PubMed  CAS  Google Scholar 

  7. Ghosh S, Kobayashi N, Nagashima S, Chawla-Sarkar M, Krishnan T, Ganesh B, Naik TN (2010) Full genomic analysis and possible origin of a porcine G12 rotavirus strain RU172. Virus Genes 40:382–388

    Article  PubMed  CAS  Google Scholar 

  8. Ghosh S, Varghese V, Samajdar S, Bhattacharya SK, Kobayashi N, Naik TN (2006) Molecular characterization of a porcine Group A rotavirus strain with G12 genotype specificity. Arch Virol 151:1329–1344

    Article  PubMed  CAS  Google Scholar 

  9. Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Palombo EA, Iturriza-Go′mara M, Maes P, Patton JT, Rahman M, Van Ranst M (2008) Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J. Virol 82:3204–3219

    Article  PubMed  CAS  Google Scholar 

  10. Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Ba′nyai K, Estes MK, Gentsch JR, Iturriza-Go′mara M, Kirkwood CD, Martella V, Mertens PP, Nakagomi O, Patton JT, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Desselberger U, Ranst MV (2008) Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 153:1621–1629

    Article  PubMed  CAS  Google Scholar 

  11. Matthijnssens J, Heylen E, Zeller M, Rahman M, Lemey P, Ranst MV (2010) Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Mol Biol Evol 27(10):2431–2436

    Article  PubMed  CAS  Google Scholar 

  12. Matthijnssens J, Rahman M, Ciarlet M, Van Ranst M (2008) Emerging human rotavirus genotypes. In: Palombo EA, Kirkwood CD (eds) Viruses in the Environment, Research Signpost, Trivandrum, pp 171–219

  13. Minamoto N, Oki K, Tomita M, Kinjo T, Suzuki Y (1988) Isolation and characterization of rotavirus from feral pigeon in mammalian cell culture. Epidemiol Inf 100:481–492

    Article  CAS  Google Scholar 

  14. Mukherjee A, Dutta D, Ghosh S, Bagchi P, Chattopadhyay S, Nagashima S, Kobayashi N, Dutta P, Krishnan T, Naik TN, Chawla-Sarkar M (2009) Full genomic analysis of a human group A rotavirus G9P[6] strain from Eastern India provides evidence for porcine-to-human interspecies transmission. Arch Virol 154:733–746

    Article  PubMed  CAS  Google Scholar 

  15. Parashar UD, Gibson CJ, Bresse JS, Glass RI (2006) Rotavirus and severe childhood diarrhea. Emerg Infect Dis 12:304–306

    Article  PubMed  Google Scholar 

  16. Parashar UD, Hummelman EG, Bresee JS, Miller M, Glass RI (2003) Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis 9:565–572

    Google Scholar 

  17. Phan TG, Okitsu S, Maneekarn N, Ushijima H (2007) Genetic heterogeneity, evolution and recombination in emerging G9 rotaviruses. Infect Genet Evol 7:656–663

    Article  PubMed  CAS  Google Scholar 

  18. Rahman M, Matthijnssens J, Goegebuer T, De Leener K, Vanderwegen L, Van der Donck I, Van Hoovels L, De Vos S, Azim T, Van Ranst M (2005) Predominance of rotavirus G9 genotype in children hospitalized for rotavirus gastroenteritis in Belgium during 1999–2003. J Clin Virol 33:1–6

    Article  PubMed  Google Scholar 

  19. Rahman M, Matthijnssens J, Yang X, Delbeke T, Arijs I, Taniguchi K, Iturriza-Go′mara M, Iftekharuddin N, Azim T, Ranst MV (2007) Evolutionary history and global spread of the emerging G12 human rotaviruses. J. Virol 81:2382–2390

    Article  PubMed  CAS  Google Scholar 

  20. Santos N, Hoshino Y (2005) Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15:29–56

    Article  PubMed  Google Scholar 

  21. Ursu K, Kisfali P, Rigo D, Ivanics E, Erdelyi K, Dan A, Melegh B, Martella V, Banyai K (2009) Molecular analysis of the VP7 gene of pheasant rotaviruses identifies a new genotype, designated G23. Arch Virol 154:1365–1369

    Article  PubMed  CAS  Google Scholar 

  22. Wang YH, Kobayashi N, Zhou DJ, Yang ZQ, Zhou X, Peng JS et al (2007) Molecular epidemiologic analysis of group A rotaviruses in adults and children with diarrhea in Wuhan City, Mainland China, 2000–2006. Arch Virol 152:669–685

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We appreciate the help of professor Jelle Matthijnssens at Rega Institute for Medical Research in Belgium for the classification of NMTL genotypes. This work was supported by a grant from the National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences.

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Authors and Affiliations

  1. Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, No.427 Maduan Street, Nangang District, Harbin, 150001, People’s Republic of China

    Hongyan Shi, Jianfei Chen, Dongbo Sun, Chengbao Wang & Li Feng

  2. Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, No.427 Maduan Street, Nangang District, Harbin, 150001, People’s Republic of China

    Huixin Li

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  1. Hongyan Shi
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Corresponding author

Correspondence to Li Feng.

Electronic supplementary material

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Fig. 1 Phylogenetic analysis of the VP7 nt sequences of strain NMTL (JF781163), indicating its genetic relationship to lineage-VI G9 strains. The tree was rooted with the OSU (G5) strain as outgroup. Abbreviations used for sequence analysis are as follows: hum, human; por, porcine; and out, outgroup.

Fig. 2 Phylogenetic analysis of the VP6 nt sequences of strain NMTL (JF781162), indicating itsgenetic relationship to strains representing the 10 I-genotypes. Abbreviations used for sequence analysis are as follows: hum, human; por, porcine; avi, avian; mur, murine; sim, simian; ovi, ovine; and equ, equine.

Fig. 3 Phylogenetic analysis of the VP1 nt sequences of strain NMTL (JF781158), indicating its genetic relationship to strains representing the four R-genotypes. Abbreviations used for sequence analysis are as follows: hum, human; por, porcine; avi, avian; sim, simian; and bov, bovine.

Fig. 4 Phylogenetic analysis of the VP3 nt sequences of strain NMTL (JF781160), indicating its genetic relationship to strains representing the six M-genotypes. Abbreviations used for sequence analysis are as follows: hum, human; por, porcine; avi, avian; sim, simian; ovi, ovine; and equ, equine.

Fig. 5 Phylogenetic analysis of the NSP1 nt sequences of strain NMTL (JF781164), indicating its genetic relationship to strains representing the 14 A-genotypes. Abbreviations used for sequence analysis are as follows: hum, human; por, porcine; avi, avian; mur, murine; sim, simian; ovi, ovine; equ, equine; and bov, bovine.

Fig. 6 Phylogenetic analysis of the NSP3 nt sequences of strain NMTL (JF781166), indicating its genetic relationship to strains representing the six T-genotypes. Abbreviations used for sequence analysis are as follows: hum, human; por, porcine; avi, avian; and sim, simian.

Fig. 7 Phylogenetic analysis of the NSP5 nt sequences of strain NMTL (JF781168), indicating its genetic relationship to strains representing the six H-genotypes. Abbreviations used for sequence analysis are as follows: hum, human; por, porcine; avi, avian; sim, simian; ovi, ovine; and bov, bovine.

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Shi, H., Chen, J., Li, H. et al. Molecular characterization of a rare G9P[23] porcine rotavirus isolate from China. Arch Virol 157, 1897–1903 (2012). https://doi.org/10.1007/s00705-012-1363-2

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  • DOI: https://doi.org/10.1007/s00705-012-1363-2

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