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Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2

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Abstract

Virulent Cronobacter sakazakii bacteriophage ES2 was isolated from swine fecal samples, and the genome sequence by was determined GS-Flx. Bacteriophage ES2 had a double-stranded DNA genome with a length of 22,162 bp and a G+C content of 50.08%. The morphological characteristics under a transmission electron microscope indicated that bacteriophage ES2 belongs to the family Myoviridae. The structural proteins, including the phage coat protein, were separated by SDS-PAGE and identified by Q-TOF. Bioinformatics analysis of the bacteriophage genome revealed 30 putative open reading frames (ORFs). The predicted protein products of the ORFs were determined and described. To our knowledge, the genome of the newly isolated bacteriophage ES2 was not significantly similar to that of any previously reported bacteriophages of members of the family Enterobacteriaceae.

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Acknowledgments

This research was supported financially by Korea Institute of Planning and Evolution for Biotechnology of Food, Agriculture, Forestry and Fisheries funded by Korean Government (108147-02-1-SB010).

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Kyungwon University, Sungnam-Si, Kyunggi-Do, 461-701, South Korea

    Young-Duck Lee & Jong-Hyun Park

  2. College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Sungbuk-Gu, Seoul, 136-701, South Korea

    Young-Duck Lee & Hyo-Ihl Chang

Authors
  1. Young-Duck Lee
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  2. Jong-Hyun Park
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  3. Hyo-Ihl Chang
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Corresponding authors

Correspondence to Jong-Hyun Park or Hyo-Ihl Chang.

Additional information

J.-H. Park and H.-I. Chang contributed equally to this work.

The complete genome sequence of bacteriophage ES2 was deposited in the GenBank database under Accession Number JF314845.

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Lee, YD., Park, JH. & Chang, HI. Genomic sequence analysis of virulent Cronobacter sakazakii bacteriophage ES2. Arch Virol 156, 2105–2108 (2011). https://doi.org/10.1007/s00705-011-1096-7

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  • DOI: https://doi.org/10.1007/s00705-011-1096-7

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