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Host cell targets of tegument protein VP22 of herpes simplex virus 1

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Abstract

In the present study, seven novel host cell factors associated with VP22 were identified from the human leukocyte cDNA library using a yeast two-hybrid high-throughput screening system. To confirm some of the interactions, VP22 and its homologues and two candidate targets were tagged with enhanced cyan or yellow fluorescent protein. While RING finger protein 10 (RNF10), WD repeat-containing protein 42A (WDR42A) or VP22 alone showed distinct subcellular localization patterns, RNF10 and WDR42A were relocated when co-expressed with VP22 or its homologues. Thus, these potential host cell factors of VP22 might expand the list of the host targets of VP22.

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Acknowledgments

This work was supported by grants from the Startup Fund of the Hundred Talents Program of the Chinese Academy of Sciences (20071010-141) and National Natural Science Foundation of China (30900059; 81000736). We thank Dr. Zhengfei Liu, Dr. Rui Zhou and Dr. Hua Zhu for the generous gift of viruses BHV-1, PRV and VZV, respectively. We thank Dr. Hong Guo, Shengping Huang and Jingjing Chen for technical assistance.

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Authors and Affiliations

  1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Meili Li, Lin Wang & Chunfu Zheng

  2. The Department of Veterinary Medicine, Beijing Agricultural College, Beijing, 102206, China

    Xiaoming Ren

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  1. Meili Li
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  2. Lin Wang
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  3. Xiaoming Ren
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  4. Chunfu Zheng
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Correspondence to Xiaoming Ren or Chunfu Zheng.

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Li, M., Wang, L., Ren, X. et al. Host cell targets of tegument protein VP22 of herpes simplex virus 1. Arch Virol 156, 1079–1084 (2011). https://doi.org/10.1007/s00705-011-0960-9

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  • DOI: https://doi.org/10.1007/s00705-011-0960-9

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