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Tomato necrotic ringspot virus, a new tospovirus isolated in Thailand

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Abstract

A new tospovirus isolated from naturally infected tomato plants grown in Nakhon Pathom province (Thailand) was characterized. Infected plants showed symptoms consisting of necrotic spots, necrotic ringspots and stem necrosis. This virus was detected using general antibodies that could recognize watermelon silver mottle virus (WSMoV), capsicum chlorosis virus (CaCV) and melon yellow spot virus (MYSV). However, it did not react with specific monoclonal antibodies (MAbs) to WSMoV and CaCV or a specific MAb to MYSV. The complete nucleotide sequences of S and M RNAs of the virus were determined. They were 3,023 and 4,716 nucleotides in length, respectively, and contained two ORFs in an ambisense arrangement. Sequence analysis indicated that amino acid sequence of the N protein shared 58.2%, 56.0% and 51.8% identity with those of CaCV, WSMoV and MYSV, respectively. The virus was experimentally transmitted by Thrips palmi and Ceratothripoides claratris. Based on our results, we conclude that this tospovirus isolate should be considered a member of a new species. The name tomato necrotic ringspot virus (TNRV) is proposed for this tospovirus.

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References

  1. Adam B, Yeh SD, Reddy DVR, Green SK (1993) Serological comparison of tospovirus isolates from Taiwan and India with impatiens necrotic spot virus and different tomato spotted wilt virus isolates. Arch Virol 130:237–250

    Article  CAS  PubMed  Google Scholar 

  2. Bezerra IC, Resende RO, Pozzer L, Nagata T, Kormelink R, de Avila AC (1999) Increase of tospoviral diversity in Brazil, with the identification of two new tospovirus species, one from Chrysanthemum and another from Zucchini. Phytopathology 89:823–830

    Article  CAS  PubMed  Google Scholar 

  3. Chen CC, Chen TC, Lin YH, Yeh SD, Hsu HT (2005) A chlorotic spot disease on calla lilies (Zantedeschia spp.) is caused by a tospovirus serologically but distantly related to Watermelon silver mottle virus. Plant Dis 89:440–445

    Article  CAS  Google Scholar 

  4. Chen CC, Chiu RJ (1996) A tospovirus infecting peanut in Taiwan. Acta Hortic 431:57–67

    Google Scholar 

  5. Chen CC, Lu YY, Cheng YH, Li JT, Yeh YC, Kang YC, Chang CP, Huang LH, Peng JC, Yeh SD (2010) Serological relationship between Melon yellow spot virus and Watermelon silver mottle virus and differential detection of the two viruses in cucurbits. Arch Virol 155:1085–1095

    Article  CAS  PubMed  Google Scholar 

  6. Chu FH, Chao CH, Peng YC, Lin SS, Chen CC, Yeh SD (2001) Serological and molecular characterization of Peanut chlorotic fan-spot virus, a new species of the genus Tospovirus. Phytopathology 91:856–863

    Article  CAS  PubMed  Google Scholar 

  7. Chiemsombat P, Gajanandana O, Warin N, Hongprayoon R, Bhunchoth A, Pongsapich P (2008) Biological and molecular characterization of tospoviruses in Thailand. Arch Virol 153:571–577

    Article  CAS  PubMed  Google Scholar 

  8. Ciuffo M, Kurowski C, Vivoda E, Copes B, Masenga V, Falk BW, Turina M (2009) A New Tospovirus sp. in Cucurbit Crops in Mexico. Plant Dis 93:467–474

    Article  CAS  Google Scholar 

  9. Ciuffo M, Tavella L, Pacifico D, Masenga V, Turina M (2008) A member of a new Tospovirus species isolated in Italy from wild buckwheat (Polygonum convolvulus). Arch Virol 153:2059–2068

    Article  CAS  PubMed  Google Scholar 

  10. Cortes I, Livieratos IC, Derks A, Peters D, Kormelink R (1998) Molecular and serological characterization of Iris yellow spot virus, a new and distinct Tospovirus species. Phytopathology 88:1276–1282

    Article  CAS  PubMed  Google Scholar 

  11. Cortes I, Saajer J, Wongjkaew KS, Pereire AM, Goldbach R, Peters D, Kormelink R (2001) Identification and characterization of a novel Tospovirus species using RT-PCR approach. Arch Virol 146:265–278

    Article  Google Scholar 

  12. De Avila AC, de Haan P, Kormelink R, Resende RO, Goldbach RW, Peters D (1993) Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. J Gen Virol 74:153–159

    Article  PubMed  Google Scholar 

  13. De Haan P, Kormelink R, Resende RO, van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. J Gen Virol 71:2207–2216

    Article  Google Scholar 

  14. De Haan P, Wagemakers L, Peters D, Goldbach R (1990) The S RNA segment of Tomato spotted wilt virus has an ambisense character. J Gen Virol 71:1001–1007

    Article  PubMed  Google Scholar 

  15. Dong JH, Cheng XF, Yin YY, Fang Q, Ding M, Li TT, Zhang LZ, Su XX, McBeath JH, Zhang ZK (2008) Characterization of tomato zonate spot virus, a new tospovirus in China. Arch Virol 153:855–864

    Article  CAS  PubMed  Google Scholar 

  16. Elliott RM (1990) Molecular biology of the Bunyaviridae. J Gen Virol 71:501–522

    Article  CAS  PubMed  Google Scholar 

  17. Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Amsterdam, pp 712–716

    Google Scholar 

  18. Francki RIB, Fauquet CM, Knudson DL, Brown F (1991) Classification and nomenclature of viruses: V report of international Committee on Taxonomy of Viruses. Arch Virol Supplement 2:1–450

    Google Scholar 

  19. Gajanandana O, Seepiban C, Pansa-ard S, Bhunchot A, Warin A, Himananto O, Pongsapic P, Chiemsombat P, Honprayoon R (2006) Production of antibodies for detection of tospoviruses in Thailand. Proceedings of 44th Kasetsart University Annual Conference, 30 January-2 February. Kasetsart University, Bangkok, pp 556–563

    Google Scholar 

  20. German TL, Ullman DE, Moyer JW (1992) Tospoviruses: diagnosis, molecular biology, phylogeny, and vector relationships. Annu Rev Phytopathology 30:315–348

    Article  CAS  Google Scholar 

  21. Goldbach R, Kuo G (1996) Introduction: proceedings of the international symposium on tospovirus and thrips of floral and vegetable crops. Acta Hortic 431:21–26

    Google Scholar 

  22. Hassani-Mehraban A, Botermans M, JThJ Verhoeven, Meekes E, Saaijer J, Peters D, Goldbach R, Kormelink R (2010) A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia. Arch Virol 155:423–428

    Article  CAS  PubMed  Google Scholar 

  23. Hassani-Mehraban A, Saijer J, Peters D, Goldbach R, Kormelink R (2005) A new tomato-infecting tospovirus from Iran. Phytopathology 95:852–858

    Article  CAS  PubMed  Google Scholar 

  24. Jain RK, Pappu HR, Pappu SS, Reddy MK, Vani A (1998) Watermelon bud necrosis tospovirus is a distinct virus species belonging to serogroup IV. Arch Virol 143:1637–1644

    Article  CAS  PubMed  Google Scholar 

  25. Jones DR (2005) Plant viruses transmitted by thrips. Eur J Plant Pathol 113:119–157

    Article  Google Scholar 

  26. Kato K, Hanada K (2000) Characterization of the S RNA segment of melon yellow spot virus. Jpn J Phytopathol 66:252–254

    CAS  Google Scholar 

  27. Knierim D, Blawid R, Maiss E (2006) The complete nucleotide sequence of a Capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand. Arch Virol 151:1761–1782

    Article  CAS  PubMed  Google Scholar 

  28. Kormelink R, de Haan P, Meurs C, Peters D, Goldbach R (1992) The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. J Gen Virol 73:2795–2804

    Article  CAS  PubMed  Google Scholar 

  29. Kormelink R, Kitajima BW, de Haan P, Zuidema D, Peters D, Goldbach R (1991) The nonstructural protein (NSs) encoded by the ambisense S RNA segment of tomato spotted wilt virus is associated with fibrous structures in infected plant cells. Virology 181:459–468

    Article  CAS  PubMed  Google Scholar 

  30. Lakshmi KV, Wightman lA, Reddy DVR, Ranga Rao GV, Buiel AAM, Reddy DDR (1995) Transmission of peanut bud necrosis virus by Thrips palmi in India. In: Parker BL, Skinner M, Lewis T (eds) Thrips Biology and Management. Plenum Press, New York, pp 179–184

    Google Scholar 

  31. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  32. Law MD, Speck J, Moyer JW (1991) Nucleotide sequence of the 3-non-coding region and N gene of the S RNA of a serologically distinct tospovirus. J GenVirol 72:2597–2601

    CAS  Google Scholar 

  33. Law MD, Speck J, Moyer JW (1992) The M RNA of impatiens necrotic spot Tospovirus (Bunyaviridae) has an ambisense genomic organization. Virology 188:732–741

    Article  CAS  PubMed  Google Scholar 

  34. McMichael LA, Persley DM, Thomas JE (2002) A new tospovirus serogroup IV species infecting capsicum and tomato in Queensland, Australia. Australasian Plant Pathol 31:231–239

    Article  Google Scholar 

  35. Moyer JW (1999) Tospoviruses (Bunyaviridae). In: Granoff A, Webster RG (eds) Encyclopedia of virology. Academic press, New York, pp 1803–1807

    Google Scholar 

  36. Palmer JM, Reddy DVR, Wightman JA, Ranga Rao GV (1990) New information on the thrips vectors of tomato spotted wilt virus in groundnut crops in India. International Arachis Newsletters 7:24–25

    Google Scholar 

  37. Persley DM, Thomas JE, Sharman M (2006) Tospoviruses-an Australian perspective. Australasian Plant Pathol 35:161–180

    Article  Google Scholar 

  38. Premachandra WTSD, Borgemeister C, Maiss E, Knierim D, Poehling HM (2005) Ceratothripoides claratris, a new vector of a Capsicum chlorosis virus Isolate Infecting Tomato in Thailand. Phytopathology 95:659–663

    Article  CAS  PubMed  Google Scholar 

  39. Reddy DVR, Ratna AS, Sudarshana MR, Poul F, Kumar IK (1992) Serological relationships and purification of bud necrosis virus. a tospovirus occurring in peanut (Arachis hypogaea L.) in India. Ann Appl Biol 120:279–286

    Google Scholar 

  40. Resende RO, Posser L, Nagata I, Bezzera C, Lima MI, Kitajima EW, de Ávila AC (1996) New tospoviruses found in Brazil. Acta Hortic 431:78–79

    Google Scholar 

  41. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Laboratory Press, New York

    Google Scholar 

  42. Satyanarayana T, Gowda S, Reddy KL, Mitchell SE, Dawson WO, Reddy DVR (1998) Peanut yellow spot virus is a member of a new serogroup of tospovirus genus based on small (S) RNA sequence and organization. Arch Virol 143:353–364

    Article  CAS  PubMed  Google Scholar 

  43. Satyanarayana T, Mitchel SE, Reddy DVR, Brown S, Kresovich S, Jarret R, Nidu RA, Demski JW (1996) Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organisation and homology to other tospoviruses. Arch Virol 141:85–98

    Article  CAS  PubMed  Google Scholar 

  44. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  45. Tsompana M, Moyer JW (2008) Tospoviruses. In: Mahy BWJ, Van Regenmortel MHV (eds) Encyclopedia of virology, vol 5, 3rd edn. Elsevier, Oxford, pp 157–162

    Chapter  Google Scholar 

  46. Ullman DE, German TL, Sherwood JL, Westcot DM, Cantone FA (1993) Tospovirus replication in insect vector cells: immunocytochemical evidence that the nonstructural protein encoded by the S RNA of tomato spotted wilt tospovirus is present in thrips vector cells. Phytopathology 83:456–463

    Article  CAS  Google Scholar 

  47. Ullman DE, Sherwood JL, German TL (1997) Thrips as vectors of plant pathogens. In Lewis, T. [Ed.] Thrips as crop pests. CAB International. University Press, Cambridge, UK, pp 539–565

    Google Scholar 

  48. Whitfield AE, Ullman DE, German TL (2005) Tospovirus-thrips interactions. Ann Rev Phytopathology 43:459–489

    Article  CAS  Google Scholar 

  49. Wijkamp I, Peters D (1993) Determination of the median latent period of two tospoviruses in Frankliniella occidentalis using a novel leaf disc assay. Phytopathology 72:875–880

    Google Scholar 

  50. Yeh SD, Chang TF (1995) Nucleotide sequence of the N gene of watermelon silver mottle virus, a proposed new member of the genus Tospovirus. Phytopathology 85:58–64

    Article  CAS  Google Scholar 

  51. Yeh SD, Lin YC, Cheng YH, Jih CL, Chen MJ, Chen CC (1992) Identification of tomato spotted wilt-like virus on watermelon in Taiwan. Plant Dis 76:835–840

    Article  Google Scholar 

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Acknowledgements

This research work was supported by the Royal Golden Jubilee PhD Program (Grant PHD/0138/2547), The Thailand Research Fund. The study was supported in part by National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency. Technical advice from Prof. Thomas L. German, Department of Entomology, University of Wisconsin-Madison, USA, is thankfully acknowledged.

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Authors and Affiliations

  1. Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand

    Channarong Seepiban & Supat Attathom

  2. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, 12120, Thailand

    Oraprapai Gajanandana

  3. Department of Entomology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand

    Tipvadee Attathom

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  1. Channarong Seepiban
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  2. Oraprapai Gajanandana
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  3. Tipvadee Attathom
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  4. Supat Attathom
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Corresponding author

Correspondence to Supat Attathom.

Additional information

Note: The nucleotide sequence data reported are available in the GenBank database under the accession numbers FJ489600 (S RNA) and FJ947152 (M RNA).

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Seepiban, C., Gajanandana, O., Attathom, T. et al. Tomato necrotic ringspot virus, a new tospovirus isolated in Thailand. Arch Virol 156, 263–274 (2011). https://doi.org/10.1007/s00705-010-0856-0

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