Abstract
Bacteriophage ΦMH1, a newly isolated temperate phage from a UV-induced lysate of Leuconostoc pseudomesenteroides, has an isometric head, a noncontractile tail, and a double-stranded DNA genome with a length of 38709 bp. Bioinformatic analysis of the phage genome revealed 65 putative open reading frames (ORFs). Predicted protein products of the ORFs were determined and described. ΦMH1 can be classified as a member of the family Siphoviridae by morphology and genome structure. The phage did not show any significant similarity to other previously reported bacteriophages of Leuconostoc species. To our knowledge, this is the first report of genomic sequencing and characterization of a L. pseudomesenteroides temperate phage.
Similar content being viewed by others
References
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
Besemer J, Borodovsky M (2005) GeneMark: web software for gene finding in prokaryotes, eukaryotes and viruses. Nucleic Acids Res 33:W451–W454
Brussow H, Canchaya C, Hardt WD (2004) Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion. Microbiol Mol Biol Rev 68:560–602
Calendar R (2006) The bacteriophages, 2nd edn. Oxford University Press, Oxford
Canchaya C, Proux C, Fournous G, Bruttin A, Brussow H (2003) Prophage genomics. Microbiol Mol Biol Rev 67:238–276
Cappelli EA, Barros RR, Camello TC, Teixeira LM, Merquior VL (1999) Leuconostoc pseudomesenteroides as a cause of nosocomial urinary tract infections. J Clin Microbiol 37:4124–4126
Cheng X (1995) Structure and function of DNA methyltransferases. Annu Rev Biophys Biomol Struct 24:293–318
Duda RL, Martincic K, Hendrix RW (1995) Genetic basis of bacteriophage HK97 prohead assembly. J Mol Biol 247:636–647
Katsura I, Hendrix RW (1984) Length determination in bacteriophage lambda tails. Cell 39:691–698
Kim JF, Jeong H, Lee JS, Choi SH, Ha M, Hur CG, Kim JS, Lee S, Park HS, Park YH, Oh TK (2008) Complete genome sequence of Leuconostoc citreum KM20. J Bacteriol 190:3093–3094
Lu Z, Altermann E, Breidt F, Kozyavkin S (2010) Sequence analysis of Leuconostoc mesenteroides bacteriophage Phi1–A4 isolated from an industrial vegetable fermentation. Appl Environ Microbiol 76:1955–1966
Makarova K, Slesarev A, Wolf Y, Sorokin A, Mirkin B, Koonin E, Pavlov A, Pavlova N, Karamychev V, Polouchine N, Shakhova V, Grigoriev I, Lou Y, Rohksar D, Lucas S, Huang K, Goodstein DM, Hawkins T, Plengvidhya V, Welker D, Hughes J, Goh Y, Benson A, Baldwin K, Lee JH, Diaz-Muniz I, Dosti B, Smeianov V, Wechter W, Barabote R, Lorca G, Altermann E, Barrangou R, Ganesan B, Xie Y, Rawsthorne H, Tamir D, Parker C, Breidt F, Broadbent J, Hutkins R, O’Sullivan D, Steele J, Unlu G, Saier M, Klaenhammer T, Richardson P, Kozyavkin S, Weimer B, Mills D (2006) Comparative genomics of the lactic acid bacteria. Proc Natl Acad Sci USA 103:15611–15616
Mikkonen M, Alatossava T (1994) Characterization of the genome region encoding structural proteins of Lactobacillus delbrueckii subsp. lactis bacteriophage LL-H. Gene 151:53–59
Oh HM, Cho YJ, Kim BK, Roe JH, Kang SO, Nahm BH, Jeong G, Han HU, Chun J (2010) Complete genome sequence analysis of Leuconostoc kimchii IMSNU 11154. J Bacteriol 192:3844–3845
Rao VB, Feiss M (2008) The bacteriophage DNA packaging motor. Annu Rev Genet 42:647–681
Siezen RJ, Bayjanov J, Renckens B, Wels M, van Hijum SA, Molenaar D, van Hylckama Vlieg JE (2010) Complete genome sequence of Lactococcus lactis subsp. lactis KF147, a plant-associated lactic acid bacterium. J Bacteriol 192:2649–2650
Tholpady SS, Sifri CD, Sawyer RG, Hazen KC, Pruett TL, Bonatti H (2010) Leuconostoc pseudomesenteroides blood stream infection following liver transplantation. Ann Transplant 15:61–66
Xu J, Hendrix RW, Duda RL (2004) Conserved translational frameshift in dsDNA bacteriophage tail assembly genes. Mol Cell 16:11–21
Young R (1992) Bacteriophage lysis: mechanism and regulation. Microbiol Rev 56:430–481
Acknowledgments
This work was supported by grant C00903 from the National Research Foundation of Korea. We thank Jean Lu and Eric Altermann for kindly providing the prophage sequences of L. mesenteroides ATCC 8293 and L. citreum KM20 [11].
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Jang, S.H., Hwang, M.H. & Chang, HI. Complete genome sequence of ΦMH1, a Leuconostoc temperate phage. Arch Virol 155, 1883–1885 (2010). https://doi.org/10.1007/s00705-010-0799-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-010-0799-5