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Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates

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Abstract

The complete nucleotide sequences of five isolates of poinsettia mosaic virus (PnMV) from Japan (JN, JO1, JO2, JO4, and JO5) were determined. These isolates contained a single large open reading frame in their genomes and shared 96.6–97.8% identity at the nucleotide level and 91.3–98.1% identity at the amino acid level with two previously reported European isolates. Interestingly, the JO isolates were found to possess eight common translational frameshift sites in the interdomain region between the methyltransferase and protease domains, resulting in considerable variation in the interdomain region compared to the other isolates. This suggests that PnMV might have evolved by creating variations in its genome by such translational frameshifts.

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Acknowledgments

This work was supported by Program for Promotion of Basic Research Activities for Innovative Bioscience (PROBRAIN).

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Authors and Affiliations

  1. Laboratory of Clinical Plant Science, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

    Yukari Okano, Takuya Shiraishi, Shuichiro Takahashi & Shigetou Namba

  2. Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

    Kensaku Maejima, Masayoshi Hashimoto, Hiroko Senshu, Johji Ozeki, Ken Komatsu, Yasuyuki Yamaji & Shigetou Namba

Authors
  1. Yukari Okano
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  2. Kensaku Maejima
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  3. Takuya Shiraishi
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  4. Masayoshi Hashimoto
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  5. Hiroko Senshu
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  6. Johji Ozeki
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  7. Shuichiro Takahashi
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  8. Ken Komatsu
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  9. Yasuyuki Yamaji
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  10. Shigetou Namba
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Corresponding author

Correspondence to Shigetou Namba.

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Okano, Y., Maejima, K., Shiraishi, T. et al. Genetic heterogeneity found in the replicase gene of poinsettia mosaic virus isolates. Arch Virol 155, 1367–1370 (2010). https://doi.org/10.1007/s00705-010-0708-y

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  • DOI: https://doi.org/10.1007/s00705-010-0708-y

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