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Symptomless infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus

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Abstract

An infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus (PnMV) was generated. It consisted of 6,098 nucleotides and encoded a polyprotein of 219.5 kDa. Sequence comparisons indicated that this isolate shared 98.6% (nucleotide) and 97.1% (amino acid) identity with the previously sequenced isolate from Germany. RNA transcripts derived from this cDNA were infectious in Nicotiana benthamiana. However, plants did not present typical PnMV symptoms. Furthermore, RNA transcripts from this cDNA clone were not infectious in poinsettia. Serial propagation of this cDNA clone in N. benthamiana plants restored symptom induction in this host but did not re-establish infectivity in poinsettia.

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Acknowledgments

We are grateful to L. Salazar, T. Munthe, A. Sletten, M.W. Dees and S. Haugslien.

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Authors and Affiliations

  1. Plant Health and Plant Protection Division, The Norwegian Institute for Agricultural and Environmental Research, Hoegskoleveien 7, 1432, Aas, Norway

    Carl Spetz & Dag-Ragnar Blystad

  2. Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, Aas, Norway

    Roar Moe

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  1. Carl Spetz
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  2. Roar Moe
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  3. Dag-Ragnar Blystad
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Correspondence to Carl Spetz.

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Spetz, C., Moe, R. & Blystad, DR. Symptomless infectious cDNA clone of a Norwegian isolate of Poinsettia mosaic virus . Arch Virol 153, 1347–1351 (2008). https://doi.org/10.1007/s00705-008-0109-7

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  • DOI: https://doi.org/10.1007/s00705-008-0109-7

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