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Complete nucleotide sequence of the Toledo isolate of turnip ringspot virus

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Abstract

The complete genomic sequence of the Toledo isolate of the comovirus, turnip ringspot virus (TuRSV), was found to consist of 2 polyadenylated RNAs. RNA 1 is 6082 nucleotides long and encodes a single predicted polypeptide of 1860 amino acids. The predicted RNA 1 polyprotein contains the polypeptides for viral replication and proteolytic processing. RNA 2, that is 3985 nucleotides long, codes for a single predicted 1095 amino acid polypeptide containing the movement and coat proteins. Phylogenetic analysis indicates that TuRSV is most closely related to radish mosaic virus, and these crucifer-infecting pathogens form a distinct clade within the comoviruses.

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Acknowledgments

The authors thank Dr. John Gray (at the University of Toledo) and the University of Toledo Plant Science Research Center for their assistance. This work was supported in part by USDA ARS Specific Cooperative Agreement: 58-3607-1-193.

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Authors and Affiliations

  1. Department of Biological Sciences, The University of Toledo, 2801 West Bancroft Street, Toledo, OH, 43606, USA

    Sushant Khandekar, Jie He & Scott Leisner

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  1. Sushant Khandekar
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  2. Jie He
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  3. Scott Leisner
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Corresponding author

Correspondence to Scott Leisner.

Additional information

The nucleotide sequence data described in this work are available in the GenBank database under accession numbers FJ712026 for TuRSV RNA 1 and FJ712027 for RNA 2.

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Khandekar, S., He, J. & Leisner, S. Complete nucleotide sequence of the Toledo isolate of turnip ringspot virus. Arch Virol 154, 1917–1922 (2009). https://doi.org/10.1007/s00705-009-0523-5

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  • DOI: https://doi.org/10.1007/s00705-009-0523-5

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