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A new paramyxovirus, Tianjin strain, isolated from common cotton-eared marmoset: genome characterization and structural protein sequence analysis

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Abstract

The complete RNA genome sequence of Tianjin strain, isolated from marmoset, has been determined. The genome was 15,384 nucleotides (nt) in length, which was identical to that of Sendai virus (SeV), and consisted of six genes in the order 3′-NP-P/C-M-F-HN-L-5′. The gene junctions contained highly conserved transcription start and stop signal sequences and trinucleotide intergenic regions (IGR) similar to those of SeV. There was also an overlapping open reading frame and a conserved AnGn site (AAAAAGGG) in the P mRNA. Phylogenetic analysis based on protein sequences from other members of the family Paramyxoviridae showed that Tianjin strain belonged to the genus Respirovirus and was most closely related to SeV. Pairwise comparisons of amino acid identities and phylogenetic analysis with homologous sequences of known SeVs demonstrated that Tianjin strain represented a new evolutionary lineage of SeV.

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Acknowledgments

This work was supported in part by a grant from the National Natural Science Foundation of China (No. 30471530). We are grateful for the assistance of Shi Jian-dang at the College of Life Science at Nankai University, who provided both technical and intellectual support for this work. Open Access: This article is distributed under the terms of the Creative Commons Attribution Noncommercial License, which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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  1. Department of Microbiology, The College of Basic Medical Science, Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin, 300070, China

    L.-Y. Shi, M. Li, L.-J. Yuan, Q. Wang & X.-M. Li

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  1. L.-Y. Shi
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  2. M. Li
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  3. L.-J. Yuan
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  5. X.-M. Li
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Correspondence to X.-M. Li.

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L.-Y. Shi and M. Li contributed equally to this work.

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Shi, LY., Li, M., Yuan, LJ. et al. A new paramyxovirus, Tianjin strain, isolated from common cotton-eared marmoset: genome characterization and structural protein sequence analysis. Arch Virol 153, 1715–1723 (2008). https://doi.org/10.1007/s00705-008-0184-9

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