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Characterization of antibody responses against a neutralizing epitope on the glycoprotein E2 of classical swine fever virus

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Abstract

The sequence TAVSPTTLR is a conserved and linear neutralizing epitope on the glycoprotein E2 of classical swine fever virus. In this study, TAVSPTTLR-directed antibodies, induced either by virions or by an epitope-focused immunogen, were characterized. The results revealed that despite the same epitope specificity, the antibodies induced by different immunogens varied significantly both in the neutralizing test and in binding inhibition assays. This suggests that the protective immunity induced by this epitope is due to more than simply the epitope specificity and that this epitope might need essential contributions from its flanking context to induce functional epitope-specific antibodies.

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Acknowledgments

This work was funded by the NSFC30221003 and the Beijing Feikai Biotech Ltd. We thank Prof. Zai-Shi Wang and Mr. Lei Wu (China Institute of Veterinary Drug Control, People’s Republic of China) for kind help in the guidance of animal manipulation. We thank Xiao-Yi Cao for the preparation of the flow diagram in Fig. 1.

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Authors and Affiliations

  1. Laboratory of Immunology, Department of Biology, Tsinghua University, Protein Science Laboratory of MOE, 100084, Beijing, People’s Republic of China

    Y. Qi, L. C. Liu, B. Q. Zhang, Z. Shen, J. Wang & Y. H. Chen

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  1. Y. Qi
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  2. L. C. Liu
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  3. B. Q. Zhang
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  4. Z. Shen
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  5. J. Wang
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Correspondence to Y. H. Chen.

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Qi, Y., Liu, L.C., Zhang, B.Q. et al. Characterization of antibody responses against a neutralizing epitope on the glycoprotein E2 of classical swine fever virus. Arch Virol 153, 1593–1598 (2008). https://doi.org/10.1007/s00705-008-0147-1

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  • DOI: https://doi.org/10.1007/s00705-008-0147-1

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