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Development of a serotyping ELISA system for Thailand virus infection

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Abstract

To distinguish Thailand virus infection from infections with other hantaviruses, we established an ELISA serotyping system using a truncated nucleocapsid protein of Thailand virus lacking 49 amino acids at the N-terminus. In evaluations using patient and rodent sera, Thailand virus infection was readily distinguished from Hantaan and Seoul virus infections. Therefore, this ELISA system is an effective alternative to neutralization tests.

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References

  1. Nichol ST, Beaty BJ, Elliott RM, Goldbach R, Plyusnin A, Schmaliohn CS, Tesh RB (2005) Bunyaviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy: classification and nomenclature of viruses: eighth report of the international committee on the taxonomy of ciruses. Elsevier, San Diego, pp 695–716

    Google Scholar 

  2. Jackson AP, Charleston MA (2004) A cophylogenetic perspective of RNA-virus evolution. Mol Biol Evol 21:45–57

    Article  PubMed  CAS  Google Scholar 

  3. Schmaljohn C, Hjelle B (1997) Hantaviruses—a global disease problem. Emerg Infect Dis 3:95–104

    Article  PubMed  CAS  Google Scholar 

  4. Elwell MR, Ward GS, Tingpalapong M, LeDuc JW (1985) Serologic evidence of Hantaan-like virus in rodents and man in Thailand. Southeast Asian J Trop Med Public Health 16:349–354

    PubMed  CAS  Google Scholar 

  5. Chu YK, Rossi C, Leduc JW, Lee HW, Schmaliohn CS, Dalrymple JM (1994) Serological relationships among viruses in the hantavirus genus, family Bunyaviridae. Virology 198:196–204

    Article  PubMed  CAS  Google Scholar 

  6. Xiao S-Y, Leduc JW, Chu YK, Schmaliohn CS (1994) Phylogenetic analyses of virus isolates in the genus hantavirus, Family Bunyaviridae. Virology 198:205–217

    Article  PubMed  CAS  Google Scholar 

  7. Pattamadilok S, Lee B-H, Kumperasart S, Yoshimatsu K, Okumura M, Nakamura I, Araki K, Khoprasert Y, Dangsupa P, Panlar P, Jandrig B, Kruger DH, Klempa B, Jakel T, Schmidt J, Ulrich R, Kariwa H, Arikawa J (2006) Geographical distribution of hantaviruses in Thailand and potential human health significance of Thailand virus. Am J Trop Med Hyg 75:994–1002

    PubMed  CAS  Google Scholar 

  8. Suputthamongkol Y, Nitatpattana N, Chayakulkeeree M, Palabodeewat S, Yoksan S, Gonzalez JP (2005) Hantavirus infection in Thailand: first clinical case report. Southeast Asian J Trop Med Public Health 36:217–220

    PubMed  Google Scholar 

  9. Araki K, Yoshimatsu K, Ogino M, Ebihara H, Lundkvist A, Kariwa H, Takashima I, Arikawa J (2001) Truncated hantavirus nucleocapsid proteins for serotyping Hantaan, Seoul, and Dobrava hantavirus infections. J Clin Microbiol 39:2397–2404

    Article  PubMed  CAS  Google Scholar 

  10. Arikawa J, Takashima I, Hashimoto N (1985) Cell fusion by haemorrhagic fever with renal syndrome (HFRS) viruses and its application for titration of virus infectivity and neutralizing antibody. Arch Virol 86:303–313

    Article  PubMed  CAS  Google Scholar 

  11. Yamada T, Hjelle B, Lanzi R, Morris C, Anderson B, Jenison S (1995) Antibody responses to four corners hantavirus infections in the deer mouse (Peromyscus maniculatus): identification of an immunodominant region of the viral nucleocapsid protein. J Virol 69:1939–1943

    PubMed  CAS  Google Scholar 

  12. Yoshimatsu K, Arikawa J, Tamura M, Yoshida R, Lundkvist A, Niklasson B, Kariwa H, Azuma I (1996) Characterization of the nucleocapsid protein of Hantaan virus strain 76–118 using monoclonal antibodies. J Gen Virol 77:695–704

    Article  PubMed  CAS  Google Scholar 

  13. Yoshimatsu K, Lee BH, Araki K, Morimatsu M, Ogino M, Ebihara H, Arikawa J (2003) The multimerization of hantavirus nucleocapsid protein depends on type-specific epitopes. J Virol 77:943–952

    Article  PubMed  CAS  Google Scholar 

  14. Wang H, Yoshimatsu K, Ebihara H, Ogino M, Araki K, Kariwa H, Wang Z, Luo Z, Li D, Hang C, Arikawa J (2000) Genetic diversity of hantaviruses isolated in china and characterization of novel hantaviruses isolated from Niviventer confucianus and Rattus rattus. Virology 278:332–345

    Article  PubMed  CAS  Google Scholar 

  15. Yoshimatsu K, Arikawa J, Kariwa H (1993) Application of a recombinant baculovirus expressing hantavirus nucleocapsid protein as a diagnostic antigen in IFA test: cross reactivities among 3 serotypes of hantavirus which causes hemorrhagic fever with renal syndrome (HFRS). J Vet Med Sci 55:1047–1050

    PubMed  CAS  Google Scholar 

  16. Okumura M, Yoshimatsu K, Araki K, Lee BH, Asano A, Agui T, Arikawa J (2004) Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses. Arch Virol 149:2427–2434

    Article  PubMed  CAS  Google Scholar 

  17. Ogino M, Ebihara H, Lee B-H, Araki K, Lundkvist A, Kawaoka Y, Yoshimatsu K, Arikawa J (2003) Use of vesicular stomatitis virus pseudotypes bearing Hantaan or Seoul virus envelope proteins in a rapid and safe neutralization test. Clin Diagn Lab Immunol 10:154–160

    Article  PubMed  CAS  Google Scholar 

  18. Lundkvist A, Bjorsten S, Niklasson B, Ahlborg N (1995) Mapping of B-cell determinants in the nucleocapsid protein of Puumala virus: definition of epitopes specific for acute immunoglobulin G recognition in humans. Clin Diagn Lab Immunol 2:82–86

    PubMed  CAS  Google Scholar 

  19. Lundkvist A, Meisel H, Koletzki D, Lankinen H, Cifire F, Geldmacher A, Sibold C, Gott P, Vaheri A, Kruger DH, Ulrich R (2002) Mapping of B-cell epitopes in the nucleocapsid protein of Puumala hantavirus. Viral Immunol 15:177–192

    Article  PubMed  CAS  Google Scholar 

  20. Yashina LN, Patrushev NA, Ivanov LI, Slonova RA, Mishin VP, Kompanez GG, Zdanovskaya NI, Kuzina II, Safronov PF, Chizhikov VE, Schmaljohn C, Netesov SV (2000) Genetic diversity of hantaviruses associated with hemorrhagic fever with renal syndrome in the far east of Russia. Virus Res 70:31–44

    Article  PubMed  CAS  Google Scholar 

  21. Lokugamage K, Kariwa H, Hayasaka D, Cui BZ, Iwasaki T, Lokugamage N, Ivanov LI, Volkov VI, Demenev VA, Slonova R, Kompanets G, Kushnaryova T, Kurata T, Maeda K, Araki K, Mizutani T, Yoshimatsu K, Arikawa J, Takashima I (2002) Genetic characterization of hantaviruses transmitted by the Korean field mouse (Apodemus peninsulae), far east Russia. Emerg Infect Dis 8:768–776

    PubMed  CAS  Google Scholar 

  22. Baek LJ, Kariwa H, Lokugamage K, Yoshimatsu K, Arikawa J, Takashima I, Kang JI, Moon SS, Chung SY, Kim EJ, Kang HJ, Song KJ, Klein TA, Yanagihara R, Song JW (2006) Soochong virus: an antigenically and genetically distinct hantavirus isolated from Apodemus peninsulae in Korea. J Med Virol 78:290–297

    Article  PubMed  CAS  Google Scholar 

  23. Miyamoto H, Kariwa H, Araki K, Lokugamage K, Hayasaka D, Cui BZ, Lokugamage N, Ivanov LI, Mizutani T, Iwasa MA, Yoshimatsu K, Arikawa J, Takashima I (2003) Serological analysis of hemorrhagic fever with renal syndrome (HFRS) patients in far eastern Russia and identification of the causative hantavirus genotype. Arch Virol 148:1543–1556

    Article  PubMed  CAS  Google Scholar 

  24. Reynes JM, Soares JL, Hue T, Bouloy M, Sun S, Kruy SL, Flye Sainte Marie F, Zeller H (2003) Evidence of the presence of Seoul virus in Cambodia. Microbes Infect 5:769–773

    Article  PubMed  Google Scholar 

  25. Plyusnina A, Ibrahim IN, Winoto I, Porter KR, Gotama IB, Lundkvist A, Vaheri A, Plyusnin A (2004) Identification of Seoul hantavirus in Rattus norvegicus in Indonesia. Scand J Infect Dis 36:356–359

    Article  PubMed  CAS  Google Scholar 

  26. Truong T-T, Truong U-N, Yoshimatsu K, Lee B-H, Araki K, Arikawa J (2004) Report of serology hantavirus in human and rodent at vietnam in 2003. In: The 6th international conference on hemorrhagic fever with renal syndrome (HFRS), hantavirus pulmonary syndrome (HPS) and hantaviruses. Seoul, p 146

  27. Chandy S, Mitra S, Sathish N, Vijayakumar TS, Abraham OC, Jesudason MV, Abraham P, Yoshimatsu K, Arikawa J, Sridharan G (2005) A pilot study for serological evidence of hantavirus infection in human population in south India. Indian J Med Res 122:211–215

    PubMed  CAS  Google Scholar 

  28. Clement J, Maes P, Muthusethupathi M, Nainan G, van Ranst M (2006) First evidence of fatal hantavirus nephropathy in India, mimicking leptospirosis. Nephrol Dial Transplant 21:826–827

    Article  PubMed  Google Scholar 

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Acknowledgments

This study was supported by a grant from the twenty-first century COE Program of Excellence for Zoonosis Control from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. We would also like to acknowledge Textcheck (English consultants) for revising the grammar of the final draft.

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Authors and Affiliations

  1. Research Center for Zoonosis Control, Hokkaido University, N20W10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan

    Ichiro Nakamura

  2. Laboratory of Animal Experimentation, Graduate School of Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo, Hokkaido, 060-8638, Japan

    Ichiro Nakamura, Kumiko Yoshimatsu, Byoung-Hee Lee, Megumi Okumura, Midori Taruishi, Koichi Araki & Jiro Arikawa

  3. Laboratory of Public Health, Department of Environmental Veterinary Science, Hokkaido University Graduate School of Veterinary Medicine, Sapporo, Hokkaido, 060-0018, Japan

    Ichiro Nakamura, Hiroaki Kariwa & Ikuo Takashima

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  1. Ichiro Nakamura
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  2. Kumiko Yoshimatsu
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  3. Byoung-Hee Lee
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  4. Megumi Okumura
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  5. Midori Taruishi
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  7. Hiroaki Kariwa
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  8. Ikuo Takashima
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  9. Jiro Arikawa
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Correspondence to Jiro Arikawa.

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Nakamura, I., Yoshimatsu, K., Lee, BH. et al. Development of a serotyping ELISA system for Thailand virus infection. Arch Virol 153, 1537–1542 (2008). https://doi.org/10.1007/s00705-008-0128-4

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  • DOI: https://doi.org/10.1007/s00705-008-0128-4

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