Summary.
For reliable diagnosis of porcine teschovirus (PTV) infection we created an RT-PCR-based molecular strategy for serotyping that encompassed the dominant neutralizing antigenic site of PTV, followed by phylogenetic analyses of amplicons. We identified neutralizing antigenic sites of PTV-1 Talfan strain through epitope mapping of neutralizing monoclonal antibodies (MAbs), using synthetic peptides spanning the capsid proteins. All 11 MAbs obtained recognized peptides in the EF loop (“puff” of VP2 protein. Two MAbs concurrently reacted to peptides, one in the GH loop of VP1 and one in the VP1 C terminus. Three-dimensional modeling of Talfan capsid protein predicted exposure of all these sites on the virion surface in a close line centered around puff. We then designed a single pair of degenerate primers to VP2 and amplified the region of ∼320 bp encompassing puff in 8 PTV prototype strains and 6 field isolates. Phylogenetic analyses of the puff sequences of 11 prototype strains and 34 field isolates obtained from databanks showed that all homotypic strains (both field and prototype) were always monophyletic, except for one ‘untypable’ Japanese strain. This RT-PCR-based strategy appears to be a reliable surrogate for serotyping and could facilitate the diagnosis and epidemiological study of PTV infection.
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References
J Auerbach D Prager S Neuhaus U Loss KH Witte (1994) ArticleTitleGrouping of porcine enteroviruses by indirect immunofluorescence and description of two new serotypes J Vet Med Ser B 41 277–282 Occurrence Handle1:STN:280:DyaK2M7ktFOjtA%3D%3D Occurrence Handle10.1111/j.1439-0450.1994.tb00228.x
V Caro S Guillot F Delpeyroux R Crainic (2001) ArticleTitleMolecular strategy for ‘serotyping’ of human enteroviruses J Gen Virol 82 79–91 Occurrence Handle1:CAS:528:DC%2BD3MXjtVGhtQ%3D%3D Occurrence Handle11125161
M Doherty D Todd N McFerran EM Hoey (1999) ArticleTitleSequence analysis of a porcine enterovirus serotype 1 isolate: relationships with other picornaviruses J Gen Virol 80 1929–1941 Occurrence Handle1:CAS:528:DyaK1MXlt1aqtb8%3D Occurrence Handle10466788
HW Dunne JL Gobble JF Hokanson DC Kradel GR Bubash (1965) ArticleTitlePorcine reproductive failure associated with a newly identified “SMEDI” group of picorna viruses Am J Vet Res 26 1284–1297 Occurrence Handle1:STN:280:DyaF287jt1egug%3D%3D Occurrence Handle5894163
Y Fujisaki T Ikeo Y Miura S Hayashi M Moriwaki (1981) ArticleTitleIsolation of porcine enterovirus from a newborn piglet with nervous symptoms Bull Nat Inst Anim Health 82 13–18
JOJ Harding JT Done GF Kershaw (1957) ArticleTitleA transmissible polio-encephalomyelitis of pigs (Talfan disease) Vet Rec 69 824–832
E Honda A Kimata I Hattori T Kumagai T Tsuda T Tokui (1990) ArticleTitleA serological comparison of 4 Japanese isolates of porcine enteroviruses with the international reference strains Jap J Vet Sci 52 49–54 Occurrence Handle1:STN:280:DyaK3c7ovFGrtA%3D%3D
T Inoue T Suzuki K Sekiguchi (1989) ArticleTitleThe complete nucleotide sequence of swine vesicular disease virus J Gen Virol 70 919–934 Occurrence Handle10.1099/0022-1317-70-4-919 Occurrence Handle1:CAS:528:DyaK3cXntFSgtg%3D%3D Occurrence Handle2543767
H Izawa RA Bankowski JA Howarth (1962) ArticleTitlePorcine enteroviruses. I. Properties of three isolates from swine with diarrhea and one from apparently normal swine Am J Vet Res 23 1131–1141 Occurrence Handle1:STN:280:DyaF387kvVyktw%3D%3D Occurrence Handle13957139
Y Kaku S Yamada Y Murakami (1999) ArticleTitleSequence determination and phylogenetic analysis of RNA-dependent RNA polymerase (RdRp) of the porcine enterovirus 1 (PEV-1) Talfan strain Arch Virol 144 1845–1852 10.1007/s007050050709 Occurrence Handle10.1007/s007050050709 Occurrence Handle1:CAS:528:DyaK1MXnsFWhtro%3D Occurrence Handle10542031
Y Kaku A Sarai Y Murakami (2001) ArticleTitleGenetic reclassification of porcine enteroviruses J Gen Virol 82 417–424 Occurrence Handle1:CAS:528:DC%2BD3MXhtFCgsrc%3D Occurrence Handle11161281
M Kimura (1980) ArticleTitleA simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences J Mol Evol 16 111–120 10.1007/BF01731581 Occurrence Handle10.1007/BF01731581 Occurrence Handle1:CAS:528:DyaL3MXmtFSktg%3D%3D Occurrence Handle7463489
NJ Knowles LS Buckley HG Pereira (1979) ArticleTitleClassification of porcine enteroviruses by antigenic analysis and cytopathic effects in tissue culture: description of three new serotypes Arch Virol 62 201–208 10.1007/BF01317552 Occurrence Handle10.1007/BF01317552 Occurrence Handle1:STN:280:DyaL3c%2FoslSjuw%3D%3D Occurrence Handle229804
C Korth B Stierli P Streit M Moser O Schaller R Fischer W Schulz-Schaeffer H Kretzschmar A Raeber U Braun F Ehrensperger S Hornemann R Glockshuber R Riek M Billeter K Wuthrich B Oesch (1997) ArticleTitlePrion (PrPSc)-specific epitope defined by a monoclonal antibody Nature 390 74–77 10.1038/36337 Occurrence Handle10.1038/36337 Occurrence Handle1:CAS:528:DyaK2sXnt1Crtr8%3D Occurrence Handle9363892
A Krumbholz R Wurm O Scheck E Birch-Hirschfeld R Egerer A Henke P Wutzler R Zell (2003) ArticleTitleDetection of porcine teschoviruses and enteroviruses by Light Cycler real-time PCR J Virol Methods 113 51–63 10.1016/S0166-0934(03)00227-1 Occurrence Handle10.1016/S0166-0934(03)00227-1 Occurrence Handle1:CAS:528:DC%2BD3sXnsVansro%3D Occurrence Handle14500127
OH Lowry NJ Rosebrough AL Farr RJ Randall (1951) ArticleTitleProtein measurement with the Folin phenol reagent J Biol Chem 193 265–275 Occurrence Handle1:CAS:528:DyaG38XhsVyrsw%3D%3D Occurrence Handle14907713
V Madr (1996) Enterovirus encephalomyelitis (previously Teschen/Talfan diseases) OS Commission (Eds) OIE manual of standards for diagnostic tests and vaccines Office International des Epizooties Paris 481–487
RC Meyer GT Woods J Simon (1966) ArticleTitlePneumonitis in an enterovirus infection in swine J Comp Pathol 76 397–405 10.1016/0021-9975(66)90060-0 Occurrence Handle10.1016/0021-9975(66)90060-0 Occurrence Handle1:STN:280:DyaF2s7ot1Slug%3D%3D Occurrence Handle6008379
RD Page (1996) ArticleTitleTreeview: an application to display phylogenetic trees on personal computers Comput Appl Biosci 12 357–358 Occurrence Handle1:STN:280:DyaK2s%2FlvVSgtg%3D%3D Occurrence Handle8902363
Palmenberg AC (1989) Sequences of picornavirus capsid proteins. In: Semler BL, Ehrenfeld E (eds) Molecular aspects of picornavirus infection and detection. American Society for Microbiology, Washington, DC, pp 211-41
CR Pringle (1999) ArticleTitleVirus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999 Arch Virol 144 2065–2070 10.1007/s007050050728 Occurrence Handle10.1007/s007050050728 Occurrence Handle1:CAS:528:DyaK1MXotFSlurg%3D Occurrence Handle10550679
N Saitou M Nei (1987) ArticleTitleThe neighbor-joining method: a new method for reconstructing phylogenetic trees Mol Biol Evol 4 406–425 Occurrence Handle1:STN:280:DyaL1c7ovFSjsA%3D%3D Occurrence Handle3447015
TJ Smith T Baker (1999) Picornaviruses: epitopes, canyons, and pockets K Maramorosch FA Murphy AJ Shatkin (Eds) Advances in virus research NumberInSeries52 Academic Press San Diego 1–23
Stanway G, Brown F, Christian P, Hovi T, Hyypiä T, King AMQ, Knowles NJ, Lemon SM, Minor PD, Pallansch MA, Palmenberg AC, Skern T, (2005) Family Picornaviridae. In: Fauquet CM, Mayo MA, Manilof J, Desselberger U, Ball LA (eds) Virus taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses. Academic Press, San Diego, pp 757-78
T Takami T Nakayama H Kawashima Y Takei K Takekuma A Hoshika (2003) ArticleTitleDetermination of enterovirus serotype inferred from sequence analysis of PCR products J Clin Virol 26 355–359 10.1016/S1386-6532(02)00103-8 Occurrence Handle10.1016/S1386-6532(02)00103-8 Occurrence Handle1:CAS:528:DC%2BD3sXhvFaisLw%3D Occurrence Handle12637085
I Thoelen E Moes P Lemey S Mostmans E Wollants AM Lindberg AM Vandamme M Van Ranst (2004) ArticleTitleAnalysis of the serotype and genotype correlation of VP1 and the 5′ noncoding region in an epidemiological survey of the human enterovirus B species J Clin Microbiol 42 963–971 10.1128/JCM.42.3.963-971.2004 Occurrence Handle10.1128/JCM.42.3.963-971.2004 Occurrence Handle1:CAS:528:DC%2BD2cXivF2ks7c%3D Occurrence Handle15004039
JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins (1997) ArticleTitleThe ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 24 4876–4882 10.1093/nar/25.24.4876 Occurrence Handle10.1093/nar/25.24.4876
Trefny L (1930) Massive illness of swine in Teschen area. Zvoroleki Obzori: 235-36
P Tunback JA Liljqvist GB Lowhagen T Bergstrom (2000) ArticleTitleGlycoprotein G of herpes simplex virus type 1: identification of type-specific epitopes by human antibodies J Gen Virol 81 1033–1040 Occurrence Handle1:CAS:528:DC%2BD3cXisVekurs%3D Occurrence Handle10725430
EJ Usherwood AA Nash (1995) ArticleTitleLymphocyte recognition of picornaviruses J Gen Virol 76 499–508 10.1099/0022-1317-76-3-499 Occurrence Handle10.1099/0022-1317-76-3-499 Occurrence Handle1:CAS:528:DyaK2MXkt1Ggtb8%3D Occurrence Handle7534810
T Yokoyama KM Kimura Y Ushiki S Yamada A Morooka T Nakashiba T Sassa S Itohara (2001) ArticleTitleIn vivo conversion of cellular prion protein to pathogenic isoforms, as monitored by conformation-specific antibodies J Biol Chem 276 11265–11271 10.1074/jbc.M008734200 Occurrence Handle10.1074/jbc.M008734200 Occurrence Handle1:CAS:528:DC%2BD3MXjvFGjsb0%3D Occurrence Handle11152682
R Zell A Krumbholz A Henke E Birch-Hirschfeld A Stelzner M Doherty E Hoey M Dauber D Prager Rc Wurm (2000) ArticleTitleDetection of porcine enteroviruses by nRT-PCR: differentiation of CPE groups I-III with specific primer sets J Virol Methods 88 205–218 10.1016/S0166-0934(00)00189-0 Occurrence Handle10.1016/S0166-0934(00)00189-0 Occurrence Handle1:CAS:528:DC%2BD3cXlvV2ltbo%3D Occurrence Handle10960708
R Zell M Dauber A Krumbholz A Henke E Birch-Hirschfeld A Stelzner D Prager RC Wurm (2001) ArticleTitlePorcine teschoviruses comprises at least eleven distinct serotypes: molecular and evolutionary aspects J Virol 75 1620–1631 10.1128/JVI.75.4.1620-1631.2001 Occurrence Handle10.1128/JVI.75.4.1620-1631.2001 Occurrence Handle1:CAS:528:DC%2BD3MXhtVSgurc%3D Occurrence Handle11160660
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Kaku, Y., Murakami, Y., Sarai, A. et al. Antigenic properties of porcine teschovirus 1 (PTV-1) Talfan strain and molecular strategy for serotyping of PTVs. Arch Virol 152, 929–940 (2007). https://doi.org/10.1007/s00705-006-0908-7
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DOI: https://doi.org/10.1007/s00705-006-0908-7