Summary
Semicarbazide-sensitive amine oxidase (EC 1.4.3.6) acts as a vascular-adhesion protein (VAP-1), mediating the adhesion of lymphocytes to vascular endothelial cells under inflammatory conditions. The relationship between the adhesive and the enzymatic functions of SSAO have not yet been fully defined. Previous studies from this laboratory showed aminohexoses, which were neither substrates nor direct inhibitors of SSAO, bound to the enzyme as reversible inhibitors in the presence of H2O2 generated during substrate oxidation. The possibility that surface l-lysine could act similarly has been investigated in the present study. The presence of l-lysine during the oxidation of benzylamine resulted in time- and dose-dependent inhibition of SSAO activity, in a process that was dependent on the H2O2 formed during benzylamine oxidation. The possible implications of this in terms of the therapeutic uses of lysine are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
V Castillo JM Lizcano M Unzeta (1999) ArticleTitlePresence of SSAO in human and bovine meninges and microvessels Neurobiology 7 263–272 Occurrence Handle1:CAS:528:DC%2BD3cXntVyrsg%3D%3D Occurrence Handle10689384
NW Flodin (1997) ArticleTitleThe metabolic roles, pharmacology, and toxicology of lysine J Am Coll Nutr 16 7–21 Occurrence Handle1:CAS:528:DyaK2sXhsFOgu70%3D Occurrence Handle9013429
P Furst (1993) ArticleTitleDietary L-lysine supplementation: a promising nutritional tool in the prophylaxis and treatment of osteoporosis Nutrition 9 71–72 Occurrence Handle1:STN:280:DyaK3s3it1Gmuw%3D%3D Occurrence Handle8467115
RS Griffith DE Walsh KH Myrmel RW Thompson A Behforooz (1987) ArticleTitleSuccess of L-lysine therapy in frequently recurrent herpes simplex infection. Treatment and prophylaxis Dermatologica 175 183–190 Occurrence Handle10.1159/000248823 Occurrence Handle1:STN:280:DyaL1c%2FgsFensg%3D%3D Occurrence Handle3115841
A Holt DF Sharman GB Baker MM Palcic (1997) ArticleTitleA continuous spectrophotometric assay for monoamine oxidase and related enzymes in tissue homogenates Anal Biochem 244 384–392 10.1006/abio.1996.9911 Occurrence Handle10.1006/abio.1996.9911 Occurrence Handle1:CAS:528:DyaK2sXmvVSqtg%3D%3D Occurrence Handle9025956
S Jalkanen M Salmi (2001) ArticleTitleCell surface monoamine oxidases: enzymes in search of a function EMBO J 20 3893–3901 10.1093/emboj/20.15.3893 Occurrence Handle10.1093/emboj/20.15.3893 Occurrence Handle1:CAS:528:DC%2BD3MXmtlChsb8%3D Occurrence Handle11483492
CM Kirton ML Laukkanen A Nieminen M Merinen CM Stolen K Armour DJ Smith M Salmi S Jalkanen MR Clark (2005) ArticleTitleFunction-blocking antibodies to human vascular adhesion protein-1: a potential anti-inflammatory therapy Eur J Immunol 35 3119–3130 10.1002/eji.200535300 Occurrence Handle10.1002/eji.200535300 Occurrence Handle1:CAS:528:DC%2BD2MXht1ylu7rL Occurrence Handle16224816
K Koskinen PJ Vainio DJ Smith M Pihlavisto S Yla-Herttuala S Jalkanen M Salmi (2004) ArticleTitleGranulocyte transmigration through the endothelium is regulated by the oxidase activity of vascular adhesion protein-1 (VAP-1) Blood 103 3388–3395 10.1182/blood-2003-09-3275 Occurrence Handle10.1182/blood-2003-09-3275 Occurrence Handle1:CAS:528:DC%2BD2cXjslKgtr0%3D Occurrence Handle14726375
J O’Sullivan M O’Sullivan KF Tipton M Unzeta M Del Mar Hernandez GP Davey (2003) ArticleTitleThe inhibition of semicarbazide-sensitive amine oxidase by aminohexoses Biochim Biophys Acta 1647 367–371 Occurrence Handle12686159
J O’Sullivan M Unzeta J Healy MI O’Sullivan G Davey KF Tipton (2004) ArticleTitleSemicarbazide-sensitive amine oxidases: enzymes with quite a lot to do Neurotoxicology 25 303–315 10.1016/S0161-813X(03)00117-7 Occurrence Handle10.1016/S0161-813X(03)00117-7 Occurrence Handle14697905
CW Tabor H Tabor SM Rosenthal (1954) ArticleTitlePurification of amine oxidase from beef plasma J Biol Chem 208 645–661 Occurrence Handle1:CAS:528:DyaG2cXltlWiug%3D%3D Occurrence Handle13174575
KF Tipton GP Davey M Motherway (2000) Monoamine oxidase assays SJ Enna M Willias JW Ferkany (Eds) et al. Current protocols in pharmacology Wiley New York 3.6.1–3.6.42
X Wang P Pietrangeli MA Mateescu B Mondovi (1996) ArticleTitleExtended substrate specificity of serum amine oxidase: possible involvement in protein posttranslational modification Biochem Biophys Res Commun 223 91–97 10.1006/bbrc.1996.0851 Occurrence Handle10.1006/bbrc.1996.0851 Occurrence Handle1:CAS:528:DyaK28XjsFGht70%3D Occurrence Handle8660385
GG Yegutkin T Salminen K Koskinen C Kurtis MJ McPherson S Jalkanen M Salmi (2004) ArticleTitleA peptide inhibitor of vascular adhesion protein-1 (VAP-1) blocks leukocyte-endothelium interactions under shear stress Eur J Immunol 34 2276–2285 10.1002/eji.200424932 Occurrence Handle10.1002/eji.200424932 Occurrence Handle1:CAS:528:DC%2BD2cXmslanu7Y%3D Occurrence Handle15259025
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olivieri, A., Tipton, K. & O’Sullivan, J. l-lysine as a recognition molecule for the VAP-1 function of SSAO. J Neural Transm 114, 747–749 (2007). https://doi.org/10.1007/s00702-007-0682-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00702-007-0682-z