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Homologous cryopreserved amniotic membrane in the repair of myelomeningocele: preliminary experience

  • Original Article - Pediatric Neurosurgery
  • Published:
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Abstract

Objective

Surgical management of spinal dysraphism often requires the use of dural substitutes. Amniotic membrane (AM) has drawn the interest of clinicians for its valuable concentration of cytokines and factors capable of promoting wound healing, re-epithelialization, inhibiting fibrosis and regulating angiogenesis. These beneficial qualities could make AM an interesting dural substitute for spina bifida repair. In this study, we describe the use of banked homologous AM as a dural substitute for the repair of spinal dysraphism in newborns. Our purpose is to test the mechanical characteristics, as well as the safety and effectiveness of AM in preventing postoperative complications and re-tethering.

Methods

The AM patch was carefully detached from the chorion of donors undergoing caesarean section, rinsed in saline solution, and cryopreserved in liquid nitrogen. Five newborns were treated using AM: three affected by open spinal dysraphism and two by spina bifida occulta. The AM patch was used as a dural substitute with two different positions and purposes: the amnion-side down covering the placode to prevent adhesions or placed extradurally facing the dura to avoid scarring and facilitating the sliding of the dural sac itself under the extradural tissue layers.

Results

No adverse events occurred, and the surgical wounds healed without complications. MRI scans taken at 3 and 6 months after surgery showed a satisfying de-tethering of the spinal cord with no obvious evidence of new adherence formation.

Conclusions

We present a multimodal interposition technique using AM as a reconstructive and anti-adhesive tissue for the treatment of open myelomeningocele (MMC) and lipomeningocele (LMC) treatment. In our experience, AM proved its efficacy in restoring the dural sac integrity without complications. We support the use of AM as a promising dural substitute, speculating on how the use of AM could potentially change reconstructive strategies for spinal dysraphism.

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Authors and Affiliations

  1. Department of Neurosurgery, Treviso Hospital, University of Padova, Via Piazzale 1, 31100, Padova, Treviso, Italy

    Elisabetta Marton, Enrico Giordan, Giorgio Gioffrè, Giuseppe Canova, Marina Grazia Mazzucco & Pierluigi Longatti

  2. Treviso Tissue Bank Foundation, Padova, Italy

    Adolfo Paolin

Authors
  1. Elisabetta Marton
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  2. Enrico Giordan
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  3. Giorgio Gioffrè
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  4. Giuseppe Canova
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  5. Adolfo Paolin
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  6. Marina Grazia Mazzucco
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  7. Pierluigi Longatti
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Correspondence to Enrico Giordan.

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The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained for the procedure illustrated. However, no IRB approval is required per institutional policy for retrospective, single-cases in which no identifiable patient information is shared.

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Marton, E., Giordan, E., Gioffrè, G. et al. Homologous cryopreserved amniotic membrane in the repair of myelomeningocele: preliminary experience. Acta Neurochir 160, 1625–1631 (2018). https://doi.org/10.1007/s00701-018-3577-x

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  • DOI: https://doi.org/10.1007/s00701-018-3577-x

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