Abstract
Two new species under the names of Lepiota brunneopileata and L. pakistanensis are described herein based on four collections during mushroom surveys in 2021–2022 from scrubland in district Gujrat, Pakistan. Morpho-anatomy and phylogenetic placement, based on Internal Transcribed Spacer (nrITS) and Larger Subunit (nrLSU), clustered them into Lepiota sect. Lepiota and Stenosporae. Lepiota brunneopileata is characterized by brown to dark brown disk, squamules on light brown pileus surface as well as on stipe, spurred, truncate basidiospores (3.9–7.0 × 2.7–4.1 μm), narrowly clavate to cylindrical and utriform cheilocystidia, with light brown to brown trichodermal pileus covering elements. Lepiota pakistanensis possesses small basidiomata with ochraceous disk, ochraceous to light brown cottony squamules on creamy white pileus surface, fusiform to slightly amygdaliform basidiospores (10.3–11.7 × 4.7–5.8 μm), clavate to broadly clavate cheilocystidia, trichodermal pileus covering with long cylindrical to fusiform terminal elements and spherocystous to clavate basal elements.
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Acknowledgements
The authors are thankful to Mr. Amanat Hussain, Mr. Jawad Amanat, Mr. Fawad Amanat and Mr. Muhammad Fiaz from Pakistan for helping in sampling. Dr. Else Vellinga (University of California, Berkeley, USA) is also acknowledged for her critical review of pre-submitted manuscript and for her valuable comments and useful suggestions.
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Authors' contributions: All authors contributed to the study’s conception and design. Samples and morpho-anatomical data were collected and studied by Amatu Rehman. Material preparation and phylogenetic analyses were performed by Amatu Rehman and Muhammad Usman. The first draft of the manuscript was written by Amatu Rehman, and all authors commented on previous versions of the manuscript. The manuscript was reviewed and edited by Muhammad Usman, Najam-ul-Sehar Afshan, and Abdul Nasir Khalid. This research work is completed in the supervision of Najam-ul-Sehar Afshan and Abdul Nasir Khalid in their laboratory. All the authors read and approved the final manuscript.
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Rehman, A., Usman, M., Afshan, N.U.S. et al. Taxonomy and phylogeny reveal two novel species of genus Lepiota (Agaricaceae, Agaricales) from Punjab, Pakistan. Plant Syst Evol 310, 8 (2024). https://doi.org/10.1007/s00606-024-01891-5
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DOI: https://doi.org/10.1007/s00606-024-01891-5