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Conservation genetics of the endangered terrestrial orchid Liparis japonica in Northeast China based on AFLP markers

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Abstract

Levels of genetic diversity and population genetic structure of the rare, endangered terrestrial orchid Liparis japonica were examined for eight natural populations (n = 185) in Northeast China using six AFLP primer pairs, where this species has experienced severe habitat loss and fragmentation. Based on 406 DNA bands, a high level of genetic diversity was found at the species level with the PPB of 85.47 %, while the genetic diversity at the population level was low (PPB = 47.48 %). A significantly high degree of population differentiation was found with 42.69 % variation existed among populations as measured by AMOVA, indicating potential restricted gene flow. The genetic distances between populations were independent of the corresponding geographic distances, and the genetic relationship of individuals had no significant correlation with their spatial distribution. The restricted gene flow might be impacted by reduced population size, habitat destruction and fragmentation. The results in this study suggested that habitat protection and keeping a stable environment are critical for the conservation of L. japonica species.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31100299), Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 20112103120016), China Postdoctoral Science Foundation (No. 2012M510835), Foundation of Liaoning Educational Committee (No. L2011107), and Foundation for the Youth Scholars of Shenyang Agricultural University (No. 20101004).

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  1. College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, People’s Republic of China

    Xuhui Chen, Junjie Guan, Rui Ding, Qun Zhang, Xingze Ling, Bo Qu & Lijun Zhang

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  1. Xuhui Chen
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  2. Junjie Guan
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  3. Rui Ding
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  4. Qun Zhang
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  6. Bo Qu
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Correspondence to Bo Qu.

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Chen, X., Guan, J., Ding, R. et al. Conservation genetics of the endangered terrestrial orchid Liparis japonica in Northeast China based on AFLP markers. Plant Syst Evol 299, 691–698 (2013). https://doi.org/10.1007/s00606-012-0744-z

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