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Genetic diversity and ecological differentiation in the endangered fen orchid (Liparis loeselii)

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Abstract

Liparis loeselii is a rare and endangered orchid occurring in Europe and north-east America. Genetic diversity and structure of this species in north-west France and the United Kingdom were investigated using amplified fragment length polymorphisms (AFLPs). Although clonality and autogamy are common in L. loeselii, we found moderate to important variability within populations. We observed a significant genetic differentiation between populations occurring in dune slacks and fens. This may be correlated with leaf shape as dune slack individuals are sometimes treated as the distinct variety L. loeselii var. ovata. Genetic differentiation between populations was generally low suggesting that gene flow can occur over long distances and possibly across the English Channel. These results show that populations from dune slacks and fens should be managed separately and that geographically distant populations may be equivalent.

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References

  • Arditti J, Ghani AKA (2000) Numerical and physical properties of orchid seeds and their biological implications. New Phytol 145:367–421

    Article  Google Scholar 

  • Bateman RM (2001) Evolution and classification of European orchids: insights from molecular and morphological characters. J Eur Orch 33:33–119

    Google Scholar 

  • Bennett MD, Leitch IJ (2003) Angiosperm DNA C-values database. Royal Botanic Gardens, Kew

    Google Scholar 

  • Bidartondo MI, Burghardt B, Gebauer G, Bruns TD, Read DJ (2004) Changing partners in the dark: isotopic and molecular evidence of ectomycorrhizal liaisons between forests orchids and trees. Proc R Soc London B 271:1799–1806

    Article  CAS  Google Scholar 

  • Cameron KM (2005) Leave it to the leaves: a molecular phylogenetic study of Malaxideae (Epidendroideae, Orchidaceae). Am J Bot 92:1025–1032

    Google Scholar 

  • Casgrain P, Legendre P (1999) The R package for multivariate analysis. Montreal

  • Catling PM (1980) Rain-assisted autogamy in Liparis loeselii (L.) L. C. Rich. (Orchidaceae). Bull Torrey Bot Club 107:525–529

    Article  Google Scholar 

  • Chase MW, Hills HG (1991) Silica gel: an ideal material for field preservation of leaf samples for DNA studies. Taxon 40:215–220

    Article  Google Scholar 

  • Daugherty CH, Cree A, Hay JM, Thompson MB (1990) Neglected taxonomy and continuing extinctions of tuatara (Sphenodon). Nature 347:177–179

    Article  Google Scholar 

  • Delforge P (1994) Guide des Orchidées d’Europe, d’Afrique du Nord et du Proche-Orient, 1st edn. Delachaux et Niestlé, Neuchâtel-Paris

    Google Scholar 

  • Delforge P (2001) Guide des Orchidées d’Europe, d’Afrique du Nord et du Proche-Orient, 2nd edn. Delachaux et Niestlé, Neuchâtel-Paris

    Google Scholar 

  • Ellstrand NC, Roose ML (1987) Patterns of genotypic diversity in clonal plant species. Am J Bot 74:123–131

    Article  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    PubMed  CAS  Google Scholar 

  • Fay MF, Cowan RS, Leitch IJ (2005) The effects of nuclear DNA content (C-value) on the quality and utility of AFLP fingerprints. Ann Bot 95:237–246

    Article  PubMed  CAS  Google Scholar 

  • Felsenstein J (1993) PHYLIP (Phylogeny inference package). Department of Genetics, University of Washington, Seattle

    Google Scholar 

  • Forrest AD, Hollingsworth ML, Hollingsworth PM, Sydes C, Bateman RM (2004) Population genetic structure in European populations of Spiranthes romanzoffiana set in the context of other genetic studies on orchids. Heredity 92:218–227

    Article  PubMed  CAS  Google Scholar 

  • Hedrén M (2001) Conservation priorities in Dactylorhiza, a taxonomically complex genus. Lindleyana 16:17–25

    Google Scholar 

  • Hedrén M, Fay MF, Chase MW (2001) Amplified fragment length polymorphisms (AFLP) reveal details of polyploid evolution in Dactylorhiza (Orchidaceae). Am J Bot 88:1868–1880

    Google Scholar 

  • Hendoux F, Aboucaya A, Bajon R et al (2001) Plan national de conservation du Liparis de Loesel (Liparis loeselii (L.) L.C.M. Rich.). Ministère de l’aménagement du territoire et de l’environnement

  • Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

    Google Scholar 

  • Jensen RJ, Ciofani KM, Miramontes LC (2002) Lines, outlines, and landmarks: morphometric analysis of leaves of Acer rubrum, Acer saccharinum (Aceraceae) and their hybrid. Taxon 51:475–492

    Article  Google Scholar 

  • Jones PS (1998) Aspects of the population biology of Liparis loeselii (L.) Rich. var. ovata Ridd. ex Godfery (Orchidaceae) in the dune slacks of South Wales, UK. Bot J Linn Soc 126:123–139

    Article  Google Scholar 

  • Juan A, Crespo MB, Cowan RS, Lexer C, Fay MF (2004) Patterns of variability and gene flow in Medicago citrina, an endangered endemic of islands in the western Mediterranean, as revealed by amplified fragment length polymorphism (AFLP). Mol Ecol 13:2679–2690

    Article  PubMed  CAS  Google Scholar 

  • Kalinowski ST (2004) Counting alleles with rarefaction: private alleles and hierarchical sampling designs. Conserv Genet 5:539–543

    Article  CAS  Google Scholar 

  • Lande R (1988) Genetics and demography in biological conservation. Science 241:1455–1460

    Article  PubMed  CAS  Google Scholar 

  • Luer CA (1975) The native orchids of the United States and Canada, excluding Florida. New York Botanical Garden, New York

    Google Scholar 

  • Maunder M, Cowan RS, Stranc P, Fay MF (2001) The genetic status and conservation managment of two cultivated bulb species extinct in the wild: Tecophilaea cyanocrocus (Chile) and Tulipa sprengeri (Turkey). Conserv Genet 2:193–201

    Article  Google Scholar 

  • May RM (1990) Taxonomy as destiny. Nature 347:129–130

    Article  Google Scholar 

  • Ouborg NJ, Piquot Y, van Groenendael JM (1999) Population genetics, molecular markers and the study of dispersal in plants. J Ecol 87:551–568

    Article  Google Scholar 

  • Pedersen HÆ (1998) Species concept and guidelines for infraspecific taxonomic ranking in Dactylorhiza (Orchidaceae). Nord J Bot 18:289–309

    Google Scholar 

  • Pedersen HÆ (2001) Late-flowering dune populations of Dactylorhiza incarnata (Orchidaceae): variation patterns and taxonomic inferences. Nord J Bot 21:177–186

    Google Scholar 

  • Perrière G, Gouy M (1996) WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78:364–369

    Article  PubMed  Google Scholar 

  • Pillon Y, Fay MF, Shipunov AB, Chase MW (2006) Species diversity versus phylogenetic diversity: a practical study in the taxonomically difficult genus Dactylorhiza (Orchidaceae). Biol Conserv 129:4–13

    Article  Google Scholar 

  • Purvis A, Hector A (2000) Getting the measure of biodiversity. Nature 405:212–219

    Article  PubMed  CAS  Google Scholar 

  • Qamaruz-Zaman F (2000) Conservation genetics of rare and endangered British orchids. PhD Thesis, University of Cambridge

  • Riddlesdell HJ (1907) Liparis loeselii Rich. var. ovata. J Bot 45:449

    Google Scholar 

  • Rosenberg MS (2002) PASSAGE Pattern analysis, spatial statistics, and geographical exegesis. Department of Biology, Arizona State University, Tempe

    Google Scholar 

  • Schneider S, Roessli D, Excoffier L (2000) ARLEQUIN: a software for population genetics data. Genetics and Biometry Laboratory, University of Geneva, Geneva

    Google Scholar 

  • Soltis PS, Gitzendanner MA (1999) Molecular systematics and the conservation of rare species. Conserv Biol 13:471–483

    Article  Google Scholar 

  • Squirrell J, Hollingsworth PM, Bateman RM, Tebbitt MC, Hollingsworth ML (2002) Taxonomic complexity and breeding system transitions: conservation genetics of the Epipactis leptochila complex (Orchidaceae). Mol Ecol 11:1957–1964

    Article  PubMed  CAS  Google Scholar 

  • Taylor DL, Bruns TD (1999) Populations, habitat and genetic correlates of mycorrhizal specialization in the ‘cheating’ orchids Corallorhiza maculata and C. mertensiana. Mol Ecol 8:1719–1732

    Article  Google Scholar 

  • Travis SE, Maschinski J, Keim P (1996) An analysis of genetic variation in Astragalus cremnophylax var. cremnophylax, a critically endangered plant, using AFLP markers. Mol Ecol 5:735–745

    PubMed  CAS  Google Scholar 

  • Vane-Wright RI, Humphries CJ, Williams PH (1991) What to protect? – Systematics and the agony of choice. Biol Conserv 55:235–254

    Article  Google Scholar 

  • Vekemans X (2002) AFLP-SURV. Laboratoire de Génétique et Ecologie Végétale, Université libre de Bruxelles, Bruxelles

    Google Scholar 

  • Vos P, Hogers R, Bleeker M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    PubMed  CAS  Google Scholar 

  • Wayne RK, Gittleman JL (1995) The problematic red wolf. Sci Am 273:26–31

    Article  Google Scholar 

  • Wheeler BD, Lambley PW, Geeson J (1998) Liparis loeselii (L.) Rich. in eastern England: constraints on distribution and population development. Bot J Linn Soc 126:141–158

    Article  Google Scholar 

  • World Checklist of Monocots (2004) The Board of Trustees of the Royal Botanic Gardens, Kew

  • Yeh FC, Yang R, Boyle T (1999) POPGENE, microsoft window-based freeware for populations genetic analysis. University of Alberta, Edmonton

    Google Scholar 

Download references

Acknowledgements

This work was partly funded by the operational programme for the Franco-British area of cross-border co-operation interreg III A. The authors wish to thank Adeline Courseaux, Cécile Godé, Glenda Willems, Pierre Saumitou-Laprade and Robyn Cowan for help and advice in the laboratory. Jean-François Arnaud, Stéphane Fénart, Maxime Pauwels and Xavier Vekemans also provided valuable help with data analyses.

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Author notes

  1. Yohan Pillon

    Present address: Laboratoire de Botanique, Institut de Recherche pour le Développement, BP A5, 98848, Nouméa, New Caledonia

  2. Faridah Qamaruz-Zaman

    Present address: Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

Authors and Affiliations

  1. G.E.P.V.: Laboratoire de Génétique et Evolution des Populations Végétales, Bâtiment SN2, Université des Sciences et Technologies de Lille 1, 59655, Villeneuve d’Ascq Cedex, France

    Yohan Pillon & Yves Piquot

  2. Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3DS, UK

    Faridah Qamaruz-Zaman & Michael F. Fay

  3. Conservatoire Botanique National de Bailleul, Hameau de Haendries, 59270, Bailleul, France

    Frédéric Hendoux

  4. Laboratoire de Biologie et Physiologie Végétales Appliquées, Université de la Nouvelle-Calédonie, BP 4477, 98847, Nouméa, New Caledonia

    Yohan Pillon

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  1. Yohan Pillon
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Correspondence to Yohan Pillon.

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Pillon, Y., Qamaruz-Zaman, F., Fay, M.F. et al. Genetic diversity and ecological differentiation in the endangered fen orchid (Liparis loeselii). Conserv Genet 8, 177–184 (2007). https://doi.org/10.1007/s10592-006-9160-7

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  • DOI: https://doi.org/10.1007/s10592-006-9160-7

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