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Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae)

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Abstract

Turnera ulmifolia constitutes a well-studied polyploid complex with allo- and autopolyploid species ranging from 2 to 8x. Flow cytometry was used to determine nuclear DNA content, and to estimate 2C- and 1Cx-values with the aim of analysing the genome size in Turnera in terms of polyploid speciation. The 2C-value and 1Cx-value were evaluated in 12 species of the T. ulmifolia complex. Nuclear DNA content was estimated by flow cytometry of nuclei stained with propidium iodide. The 2C DNA content ranged from 1.38 to 1.83 pg in diploids, from 2.67 to 3.96 pg in tetraploids, from 2.73 to 4.31 pg in hexaploids, and from 3.53 to 5.90 pg in octoploids. The 1Cx-value ranged from 0.44 to 0.99 pg. The Turnera ulmifolia complex showed an increase in total DNA content in the ploidy level, but not in the expected proportion. The general tendency indicated a decrease in the 1Cx-value with increasing chromosome number, with T. grandidentata 4x being an outstanding exception. The 1Cx-values in the allooctoploids T. aurelii and T. cuneiformis differed by 1.6-fold from each other, probably as a result of different evolutionary histories following divergence from the last common ancestor.

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Acknowledgments

We wish to especially thank to Heather Machado for the revision of the final manuscript.

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Authors and Affiliations

  1. Instituto de Botánica Darwinion (CONICET), Labardén 200, CC 22, B1642HYD, San Isidro, Buenos Aires, Argentina

    Alicia López

  2. Facultad de Ciencias Exactas y Naturales y Agrimensura (UNNE), Instituto de Botánica del Nordeste (UNNE-CONICET), CC 209, 3400, Corrientes, Argentina

    Andrea F. Panseri & Aveliano Fernández

  3. Departamento de Ecología, Genética y Evolución (FCEN, UBA), Ciudad Universitaria, Pabellón II, Piso 4, C1428EHA, Buenos Aires, Argentina

    Lidia Poggio

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  1. Alicia López
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Correspondence to Alicia López.

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López, A., Panseri, A.F., Poggio, L. et al. Nuclear DNA content in the polyploid complex Turnera ulmifolia (Turnera L., Passifloraceae). Plant Syst Evol 296, 225–230 (2011). https://doi.org/10.1007/s00606-011-0489-0

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