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Chemical and genetic variability of selected Turkish oregano (Origanum onites L.) clones

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Abstract

Turkish oregano (Origanum onites L.) is a major aromatic plant that belongs to Labiatae family. In this work fourteen Turkish oregano clones have been characterized chemically and genetically. Essential oil obtained by hydro-distillation was characterized by gas chromatography. Genetic variation was determined by use of random amplified polymorphic DNA (RAPD) markers. Variation of essential oils in the selected clones was subjected to cluster analysis, and two chemotypes, carvacrol and thymol, were identified. Carvacrol was found to be the main component in all clones except clone-661, the main component of which was thymol. The oregano clones were divided into three main groups by clustering on the basis of RAPD markers. Genetic similarity values among the oregano clones ranged between 0.49 and 0.73 which was indicative of a low level of genetic variation. Clones originating from locations close to each other had similar RAPD markers. Correlation analysis of the genetic distance matrix and the Euclidian distance matrix revealed no significant correlation between them. The results also indicated that there is no relationship between genetic structure of the selected clones and essential oil composition.

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References

  • Arıdoğan BC, Baydar H, Kaya S, Demirci M, Özbaşar D, Mumcu E (2002) Antimicrobial activity and chemical composition of some essential oils. Arch Pharm Res 25:860–864

    Article  PubMed  Google Scholar 

  • Ayanoğlu F, Ergül A, Arslan M (2006) Assessment of genetic diversity in Turkish oregano (Origanum onites L.) germplasm by AFLP analysis. J Hortic Sci Biotech 81:45–50

    Google Scholar 

  • Azcan N, Kara M, Asilbekova DT, Ozek T, Başer KHC (2000) Lipids and essential oil of Origanum onites. Chem Nat Comp 36:132–136

    Article  CAS  Google Scholar 

  • Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils-a review. Food Chem Toxicol 46:446–475

    Article  CAS  PubMed  Google Scholar 

  • Bansleben A-C, Schellenberg I, Einax JW, Schaefer K, Ulrich D (2009) Chemometric tools for identification of volatile aroma-active compounds in oregano. Anal Bioanal Chem 395:1503–1512

    Article  CAS  PubMed  Google Scholar 

  • Baranauskiene R, Venskutonis PR, Dewettinck K, Verhe R (2006) Properties of oregano (Origanum vulgare L.), citronella (Cymbopogon nardus G.) and marjoram (Majorana hortensis L.) flavors encapsulated into milk protein-based matrices. Food Res Int 39:413–425

    Article  CAS  Google Scholar 

  • Başer KHC (2001) Her derde deva bir bitki Kekik. Tübitak Bilim ve Teknik Dergisi Mayıs 74–77

  • Baydar H, Sağdiç O, Özkan G, Karadoğan T (2004) Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control 15:169–172

    Article  CAS  Google Scholar 

  • Ceylan A, Bayram E, Geren H (1999) İzmir kekiği (Origanum onites L.) ıslahında geliştirilen klonların agronomik ve kalite özellikleri üzerinde araştırma. Turk J Agric For 23:1163–1168

    Google Scholar 

  • Ceylan A, Bayram E, Sahbaz N, Otan H, Karaman Ş (2003) Yield performance and essential oil composition of individual plants and improved clones of Origanum onites L. grown in Aegean region of Turkey. Israel J Plant Sci 51:285–290

    Article  CAS  Google Scholar 

  • D’antuono LF, Galletti GC, Bocchini P (2000) Variability of essential oil content and composition of Origanum vulgare L. populations from a North Mediterranean area (Liguria region, Northern Italy). Ann Bot 86:471–478

    Article  Google Scholar 

  • Davis PH (1982) Flora of Turkey and The East Aegean Islands, vol 7. Edinburgh University Press, Edinburgh, pp 1–948

    Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Dundar E, Olgun EG, Isiksoy S, Kurkcuoglu M, Baser KHC, Bal C (2008) The effects of intra-rectal and intra-peritoneal application of Origanum onites L. essential oil on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat. Exp Toxicol Pathol 59:399–408

    Article  CAS  PubMed  Google Scholar 

  • Echeverrigaray S, Agostini G, Atti-Serfini L, Paroul N, Pauletti GF, Atti dos Santos AC (2001) Correlation between the chemical and genetic relationships among commercial thyme cultivars. J Agric Food Chem 49:4220–4223

    Article  CAS  PubMed  Google Scholar 

  • Gounaris Y, Skoula M, Fournaraki C, Drakakaki G, Makris A (2002) Comparison of essential oils and genetic relationship of Origanum × intercedens to it’s parental taxa in the island of Crete. Biochem Syst Ecol 30:249–258

    Article  CAS  Google Scholar 

  • Günüz A, Özörgücü B (1999) An investigation on the morphology, anatomy and ecology of Origanum onites L. Turk J Bot 23:19–32

    Google Scholar 

  • Ietswaart JH (1980) A taxonomic revision of the genus Origanum (Labiatae). Leiden botanical series, vol 4. Leiden University Press, The Hague, pp 1–153

    Google Scholar 

  • Jaccard P (1908) Novelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

    Google Scholar 

  • Joshi K, Chavan P, Warude D, Patwardhan B (2004) Molecular markers in herbal drug technology. Curr Sci 87:159–165

    CAS  Google Scholar 

  • Katsiotis A, Nikoloudakis N, Linos A, Drossou A, Constantinidis T (2009) Phylogenetic relationships in Origanum spp based on rDNA sequences and intra-genetic variation of Greek O. vulgare subsp. hirtum revealed by RAPD. Sci Hortic 121:103–108

    Article  CAS  Google Scholar 

  • Kitiki A, Kesercioğlu T, Tan A, Nakiboğlu M, Otan H, Sarı O, Oğuz B (1997) Biosystematic research on some Origanum L. species in Aegean and west Mediterranean regions in Turkey. Anadolu J AARI 7:26–40

    Google Scholar 

  • Klocke E, Langbehn J, Grewe C, Pank F (2002) DNA Fingerprinting by RAPD on Origanum majorana L. J Herbs Spices Med Plants 9:171–176

    Article  CAS  Google Scholar 

  • Kokkini S (1997) Taxonomy, diversity and distribution of Origanum species. In: Padulosi S (ed) Oregano. Proceedings of the IPGRI international workshop, vol 14, Rome, Italy, pp 2–12

  • Kokkini S, Karousou R, Hanlidou E, Lanaras T (2004) Essential oil composition of Greek (Origanum vulgare ssp. hirtum) and Turkish (O. onites) oregano: a tool for their distinction. J Essent Oil Res 16:334–338

    CAS  Google Scholar 

  • Labra M, Miele M, Ledda B, Grassi F, Mazzei M, Sala M (2004) Morphological characterization, essential oil composition and DNA genotyping of Ocimum basilicum L. cultivars. Plant Sci 167:725–731

    Article  CAS  Google Scholar 

  • Mantel NA (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220

    CAS  PubMed  Google Scholar 

  • Marieschi M, Torelli A, Poli F, Sacchetti G, Bruni R (2009) RAPD-based method for the quality control of Mediterranean oregano and its contribution to pharmacognostic techniques. J Agric Food Chem 57:1835–1840

    Article  CAS  PubMed  Google Scholar 

  • Marquard R, Th Müler, Ceylan A, Bayram E, Otan H (1996) Origanum-Wildsammlungen aus der Türkei: Gehalte und Zusammen-setzung des atherischen Öls. Arznei- und Gewürzpflanzen 1:134–137

    Google Scholar 

  • Marquard R, Ceylan A, Bayram E, Weinbrenner G (1998) Auslesezüchtung bei origanum onites auf der Basis von Wild-sammlungen in der Westtürkei. Deutsch-Türkische Agrarforschung, 5. Symposium vom 29. September-4. Oktober 1997, an der Akdeniz Universitat in Antalya, Türkei. Verlag Ulrich E, Grauer, Stuttgart, pp 159–164

    Google Scholar 

  • Masi L, Siviero P, Esposito C, Castaldo D, Siano F, Laratta B (2006) Assessment of agronomic chemical and genetic variability in common basil (Ocimum basilicum L.). Eur Food Res Technol 223:273–281

    Article  Google Scholar 

  • Nhu-Trang T-T, Casabianca H, Grenier-Loustalot M-F (2006) Deuterium/hydrogen ratio analysis of thymol, carvacrol, γ-terpinene and p-cymene in thyme, savory and oregano essential oils by gas chromatography-pyrolysis-isotope ratio mass spectrometry. J Chromatogr A 1132:219–227

    Article  CAS  PubMed  Google Scholar 

  • Novak J, Lukas B, Bolzer K, Grausgruber-Groger S, Degenhardt J (2007) Identification and characterization of simple sequence repeat markers from a glandular Origanum vulgare expressed sequence tag. Mol Ecol Notes 8:599–601

    Google Scholar 

  • Olivier GW (1997) The world market of oregano. In: Padulosi S (ed) Oregano. Proceedings of the IPGRI international workshop, vol 14, Rome, Italy, pp 141–145

  • Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98:107–112

    Article  CAS  Google Scholar 

  • Rohlf FJ (1998) NTSYS-pc: numerical taxonomy and multivariate analysis system. Version 2.0, user’s guide, New York

  • Skoula M, Gotsiou P, Naxakis G, Johnson CB (1999) A chemosystematic investigation on the mono-and sesquiterpenoids in the genus Origanum (Labiatae). Phytochemistry 52:649–657

    Article  CAS  Google Scholar 

  • Sokovic M, Tzakou O, Pitarokili D, Couladis M (2002) Antifungal activities of aromatic plants growing wild in Greece. Nahrung Food 46:317–320

    Article  CAS  Google Scholar 

  • Trindade H, Costa MM, Sofia BLA, Pedro LG, Figueiredo AC, Barroso JG (2008) Genetic diversity and chemical polymorphism of Thymus caespititius from Pico, Sao Jorge and Terceira islands (Azore). Biochem Syst Ecol 36:790–797

    Article  CAS  Google Scholar 

  • Vieira RF, Grayer RJ, Paton A, Simon JE (2001) Genetic diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers. Biochem Syst Ecol 29:287–304

    Article  CAS  PubMed  Google Scholar 

  • Wichtl M (1971) Die Pharmakognostisch-chemische Analyse. Band 12, Verlagsgesellschaft Frankfurt a. M

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic marker. Nucleic Acid Res 18:6531–6535

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank the Scientific and Technical Research Council of Turkey for financial support to carry out this study (Project no: 106 O 440).

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Authors and Affiliations

  1. Department of Field Crops, Agricultural Faculty of Ege University, Bornova, Izmir, Turkey

    Fatma Aykut Tonk, Süer Yüce, Emine Bayram, R. Refika Akçali Giachino & Çiğdem Sönmez

  2. Department of Field Crops, Agricultural Faculty of Gaziosmanpaşa University, Tokat, Turkey

    İsa Telci

  3. Department of Field Crops, Agricultural Faculty of Yuzuncu Yıl University, Van, Turkey

    Mehmet Alp Furan

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  1. Fatma Aykut Tonk
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  2. Süer Yüce
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  3. Emine Bayram
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  5. Çiğdem Sönmez
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  6. İsa Telci
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Correspondence to Fatma Aykut Tonk.

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Tonk, F.A., Yüce, S., Bayram, E. et al. Chemical and genetic variability of selected Turkish oregano (Origanum onites L.) clones. Plant Syst Evol 288, 157–165 (2010). https://doi.org/10.1007/s00606-010-0320-3

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