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Genetic differentiation and postglacial migration of the Dactylorhiza majalis ssp. traunsteineri/lapponica complex into Fennoscandia

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Abstract

Eight variable regions (microsatellites, insertion/deletion and duplication regions) from the plastid DNA genome were analyzed for 91 populations belonging to Dactylorhiza majalis ssp. traunsteineri and closely related taxa. A total of 36 composite plastid haplotypes were found. The two dominating haplotypes had a clear geographic distribution suggesting at least two separate immigration routes into Scandinavia after the last glaciation: one southwestern route and one or two southeastern routes. D. majalis ssp. traunsteineri could not be clearly separated from any of the other taxa included in the study except for D. majalis ssp. sphagnicola. The morphologically similar taxa D. majalis ssp. traunsteineri, D. majalis ssp. lapponica and D. majalis ssp. russowii showed no genetic differentiation, and therefore we suggest an amalgamation of the three taxa into one broadly circumscribed subspecies; D. majalis ssp. lapponica. The plastid data also revealed incidents of hybridization and possible introgression between D. majalis ssp. lapponica and other members of the genus, e.g., D. incarnata.

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Acknowledgments

We would like to thank Sunniva Aagaard, Åse Bøilestad Breivik, Stefan Ericsson, Sven Hansson, Ursula Malm, Lennart Nordström, Tarmo Pikner, Mikko Piirainen, Mari Reitalu, David Ståhlberg, Taavo Tuulik, Kai Vahtra and Finn Wischmann for field assistance and/or collecting material. We also thank two anonymous reviewers for valuable comments. The study was supported by grants from Lunds Botaniska Förening to SN and from the Crafoord Foundation and the Swedish research council for environment, agricultural sciences and spatial planning, FORMAS (grant 2002-102) to MH.

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  1. Department of Ecology, Plant Ecology and Systematics, University of Lund, Sölvegatan 37, 223 62, Lund, Sweden

    Sofie Nordström & Mikael Hedrén

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  1. Sofie Nordström
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Correspondence to Sofie Nordström.

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Table 3

Table 3 Identification and geographic origin of populations of Dactylorhiza Neck. ex Nevski used in this study. Latitude and longitude references are approximate
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Nordström, S., Hedrén, M. Genetic differentiation and postglacial migration of the Dactylorhiza majalis ssp. traunsteineri/lapponica complex into Fennoscandia. Plant Syst Evol 276, 73–87 (2008). https://doi.org/10.1007/s00606-008-0084-1

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