Skip to main content
SpringerLink
Log in

Extensive ribosomal DNA (18S-5.8S-26S and 5S) colocalization in the North American endemic sagebrushes (subgenus Tridentatae, Artemisia, Asteraceae) revealed by FISH

  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Chromomycin A3 banding and fluorescent in situ hybridization (FISH) have been performed for six Artemisia species with special emphasis on subgenus Tridentatae. Morphometrical data on karyotype characters were calculated and idiograms with the position of GC-rich regions and 18S-5.8S-26S and 5S sites of ribosomal DNA were constructed. These sites were all colocalized. To our knowledge, this is the first time in the large family Asteraceae, indeed in angiosperms in general, that colocalization of the two rDNA regions studied is found at every single marked locus. In addition, transcriptionally active nucleolar organizer regions were detected after silver nitrate staining. Tridentatae is a cytogenetically homogeneous subgenus, which suggests that evolution of these species has not been coupled with important karyotypic reorganization. However, a few species are taxonomically difficult and show substantial differences. A loss of rDNA loci has been detected in a tetraploid taxon with respect to the diploids studied. These data provide clarifying insight into interspecific relationships between the studied taxa and overall evolutionary and systematic relationships of the Tridentatae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abd El-Twab M. H. and Kondo K. (2006). FISH physical mapping of 5S, 45S and Arabidopsis-type telomere sequence repeats in Chrysanthemum zawadskii showing intra-chromosomal variation and complexity in nature. Chromosome Bot. 1: 1–5

    Google Scholar 

  • Beetle A. A. (1959). New names within section Tridentatae of Artemisia. Rhodora 61: 82–85

    Google Scholar 

  • Beetle A. A. (1960) A study of sagebrush, the section Tridentatae of Artemisia. In: Bulletin 368, University of Wyoming experiment station. Laramie, WY.

  • Camacho J. P. M., Sharbel T. F. and Beukeboom L.W. (2000). B-chromosome evolution. Philos. Trans. Roy. Soc. Lond. Series B Biol. Sci. 355: 163–178

    Article  CAS  Google Scholar 

  • Castilho A. and Heslop-Harrison J. S. (1995). Physical mapping of 5S and 18S-25S rDNA and repetitive DNA sequences in Aegilops umbellulata. Genome 38: 91–96

    CAS  PubMed  Google Scholar 

  • Cerbah M., Coulaud J. and Siljak-Yakovlev S. (1998). rDNA organization and evolutionary relationships in the genus Hypochaeris (Asteraceae). J.Heredity 89: 312–318

    Article  CAS  Google Scholar 

  • Cronquist A. (1994) Asterales. In: Cronquist A., Holmgren N. H., Reveal J. L., Holmgren P. K. (eds.) Intermountain Flora , New York Botanical Garden, Bronx, Vol 5.

  • Cuadrado A. and Jouve N. (1994). Highly repetitive sequences in B chromosomes of Secale cereale revealed by fluorescent in situ hybridization. Genome 37: 709–712

    Article  PubMed  CAS  Google Scholar 

  • Donald T. M., Leach C. R., Clough A. and Timmis J. M. (1995). Ribosomal RNA genes and the Bchromosome of Brachycome dichromosomatica. Heredity 74: 556–561

    PubMed  CAS  Google Scholar 

  • Drouin G. and Moniz de Sa M. (1995). The concerted evolution of 5S ribosomal genes linked to the repeat units of other multigene families. Molec. Biol. Evol. 12: 481–493

    PubMed  CAS  Google Scholar 

  • Garcia S., Garnatje T., Dariimaa S., Tsooj S. and Vallès J. (2006). New or rarely reported chromosome numbers in taxa of subtribe Artemisiinae (Anthemideae, Asteraceae) from Mongolia. Bot. J. Linn. Soc. 150: 203–210

    Article  Google Scholar 

  • Garcia S., Sanz M., Garnatje T., Kreitschitz A., McArthur E. D. and Vallès J. (2004). Variation of DNA amount of 47 populations of the subtribe Artemisiinae and related taxa (Asteraceae, Anthemideae): karyological, ecological and systematic implications. Genome 47: 1004–1014

    Article  PubMed  CAS  Google Scholar 

  • Garnatje T., Vallès J., Vilatersana R., Garcia-Jacas N., Susanna A. and Siljak-Yakovlev S. (2004). Molecular cytogenetics of Xeranthemum L. and related genera (Asteraceae, Cardueae). Pl. Biol. 6: 140–146

    Article  CAS  Google Scholar 

  • Geber G. and Schweizer D. (1987). Cytochemical heterochromatin differentiation in Sinapis alba (Cruciferae) using a simple air-drying technique for producing chromosome spreads. Pl. Syst. Evol. 158: 97–106

    Article  Google Scholar 

  • Green D. M. (1988). Cytogenetics of the endemic New Zealand frog, Leiopelma hochstetteri: extraordinary supernumerary chromosome variation and a unique sex-chromosome system. Chromosoma 97: 55–70

    Article  Google Scholar 

  • Hidalgo O. (2006) El grupo Rhaponticum (Asteraceae, Cardueae, Centaureinae): delimitación y filogenia. PhD dissertation, Universitat de Barcelona, Spain.

  • Hidalgo O., Garcia-Jacas N., Garnatje T., Susana A. and Siljak-Yakovlev S. (2007). Karyological evolution in Rhaponticum (Asteraceae, Cardueae) and related genera. Bot. J. Linn. Soc. 153: 193–201

    Article  Google Scholar 

  • Kavalco K. F. and Pazza R. (2004). A rapid alternative for obtaining silver-positive patterns in chromosomes. Genet. Molec. Biol. 27: 196–198

    Google Scholar 

  • Kornkven A. B., Watson L. and Estes J. (1998). Phylogenetic analysis of Artemisia section Tridentatae (Asteraceae) based on sequences from the internal transcribed spacers (ITS) of nuclear ribosomal DNA. Amer. J. Bot. 85: 1787–1795

    Article  CAS  Google Scholar 

  • Kornkven A. B., Watson L. and Estes J. (1999). A molecular phylogeny of Artemisia sect. Tridentatae (Asteraceae) based on chloroplast DNA restriction site variation. Syst. Bot. 24: 69–84

    Article  Google Scholar 

  • Kotseruba V., Gernand D., Meister A. and Houben A. (2003). Uniparental loss of ribosomal DNA in the allotetraploid grass Zingeria trichopoda (2n=8). Genome 46: 156–163

    Article  PubMed  CAS  Google Scholar 

  • Lansdorp P. M. (2005). Major cutbacks at chromosome ends. Trends Biochem. Sci. 30: 388–395

    Article  PubMed  CAS  Google Scholar 

  • Levan A., Fredga K. and Sandberg A. A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220

    Article  Google Scholar 

  • Lim K. Y., Matyásek R., Lichtenstein C. P. and Leitch A. R. (2000). Molecular cytogenetic analyses and phylogenetic studies in the Nicotiana section Tomentosae. Chromosoma 109: 245–258

    Article  PubMed  CAS  Google Scholar 

  • Maluszynska J. and Heslop-Harrison J. S. (1991). Localization of tandemly-reteated DNA sequences in Arabidopsis thaliana. Plant J. 1: 159–166

    Article  Google Scholar 

  • McArthur E. D., Pope C. L. (1979) Karyotypes of four Artemisia species: A. carruthii, A. filifolia, A.frigida and A. spicescens. Great Basin Naturalist: 419–426.

  • McArthur E. D., Pope C. L. and Freeman D. C. (1981). Chromosomal studies of subgenus Tridentatae of Artemisia: evidence for autopolyploidy. Amer. J.Bot. 68: 589–605

    Article  Google Scholar 

  • McArthur E. D. and Sanderson S. C. (1999). Cytogeography and chromosome evolution of subgenus Tridentatae of Artemisia (Asteraceae). Amer. J.Bot. 86: 1754–1775

    Article  Google Scholar 

  • Mendelak M. and Schweizer D. (1986). Giemsa C-banded karyotypes of some diploid Artemisia species. Pl. Syst. Evol. 152: 195–210

    Article  Google Scholar 

  • Mishima M., Ohmido N., Fukui K. and Yahara T. (2002). Trends in site-number change of rDNA loci during polyploid evolution in Sanguisorba (Rosaceae). Chromosoma 110: 550–558

    PubMed  CAS  Google Scholar 

  • Muravenko O. V., Amosova A. V., Samatadze T. E., Semenova O., Nosova I. V., Popov K. V., Shostak N. G., Zoschuk S. A. and Zelenin A. V. (2004). Chromosome localization of 5S and 45S ribosomal DNA in the genomes of Linum L. species of the section Linum (syn. Protolinum and Adenolinum). Russian J. Genet. 40: 193–196

    Article  CAS  Google Scholar 

  • Ohri D. and Ahuja M. (1990). Giemsa C-banded in Quercus L. (oak). Silvae Genet. 39: 5–6

    Google Scholar 

  • Pellicer J., Garcia S., Garnatje T., Hidalgo O., Korobkov A. A., Dariimaa S. and Vallès J. (2007). Chromosome counts in Asian Artemisia L. (Asteraceae) species: from diploids to the first report of the highest polyploid in the genus. Bot. J. Linn. Soc. 153: 301–310

    Article  Google Scholar 

  • Persson K. (1974). Biosystematic studies in the Artemisia maritima complex in Europe. Opera Bot. 35: 1–188

    Google Scholar 

  • Pires J. C., Lim K. Y., Kovarík A., Matyásek R., Boyd A., Leitch A. R., Leitch I. J., Bennett M. D., Soltis P. S. and Soltis D. E. (2004). Molecular cytogenetic analysis of recently evolved Tragopogon (Asteraceae) allopolyploids reveal a karyoype that is additive of the diploid progenitors. Amer. J. Bot. 91: 1022–1035

    CAS  Google Scholar 

  • Raina S. N., Mukai Y., Kawaguchi K., Goel S. and Jain A. (2001). Physical mapping of 18S-5.8S-26S ribosomal RNA gene families in three important vetches (Vicia species) and their allied taxa constituting three species complex. Theor. Appl. Genet. 103: 839–845

    Article  CAS  Google Scholar 

  • Romero C. (1986). A new method for estimating karyotype asymmetry. Taxon 35: 526–530

    Article  Google Scholar 

  • Rossi A. R. and Gornung E. (2005). Cytogenetic analysis of three Italian populations of Coregonus lavaretus (Pisces, Salmoniformes) with chromosomal localization of major and minor ribosomal genes and telomeric repeats. Hereditas 142: 15–21

    Article  PubMed  Google Scholar 

  • Schweizer D. and Ehrendorfer F. (1983). Evolution of the C-band patterns in Asteraceae-Anthemideae. Biol. Zentralbl. 102: 637–655

    Google Scholar 

  • Sharma A. and Sen S. (2002). Chapter 1. Chromosome: structure and components. In: Sharma, A. and Sen, S. (eds) Chromosome botany, pp 1–30. Science Publishers, Inc. Enfield, New Hampshire

    Google Scholar 

  • Shultz L. M. (2005). Re-examination of subgeneric concepts in Artemisia. In: Ling, Y. R. (eds) International Symposium on Artemisia and its allies, pp 36–44. South China Institute of Botany, Guangzhou

    Google Scholar 

  • Shultz L. M. (1983) Systematics and anatomical studies of Artemisia subgenus Tridentatae (Anthemideae: Asteraceae). PhD dissertation, Claremont Graduate School, California.

  • Siljak-Yakovlev S. and Cartier D. (1986). Heterochromatin patterns in some taxa of Crepis praemorsa complex. Caryologia 39: 27–32

    Google Scholar 

  • Siljak-Yakovlev S., Cerbah M., Coulaud J., Stoian V., Brown S. C., Jelenic S. and Papes D. (2002). Nuclear DNA content, base composition, heterochromatin and rDNA in Picea omorika and Picea abies. Theor. Appl. Genet. 104: 505–512

    Article  PubMed  CAS  Google Scholar 

  • Siroky J., Lysák M. A., Doležel J., Kejnovsky E. and Vyskot B. (2001). Heterogeneity of rDNA distribution and genome size in Silene spp. Chromosome Res. 9: 387–393

    Article  PubMed  CAS  Google Scholar 

  • Sone T., Fujisawa M., Takenaka M., Nakagawa S., Yamaoka S., Sakaida1 M., Nishiyama R., Yamato K. T., Ohmido N., Fukui K., Fukuzawa H., Ohyama K. (1999). Bryophyte 5S rDNA was inserted into 45S rDNA repeat units after the divergence from higher land plants. Pl. Molec. Biol. 41: 679–685.

  • Srivastava A. K. and Schlessinger D. (1991). Structure and organization of ribosomal DNA. Biochimie 73: 631–638

    Article  PubMed  CAS  Google Scholar 

  • Stebbins G. L. (1971). Chromosomal evolution in higher plants. Ed. Arnold, London

    Google Scholar 

  • Stitou S., Jimenez R., De la Guardia R. D. and Burgos M. (2000). Sex-chromosome pairing through heterochromatin in the African rodent Lemniscomys barbarus (Rodentia, Muridae). A synaptonemal complex study. Chromosome Res. 8: 277–283

    Article  PubMed  CAS  Google Scholar 

  • Torrell M., Cerbah M., Siljak-Yakovlev S. and Vallès J. (2001). Etude cytogénétique de trois taxons du complexe d'Artemisia campestris L. (Asteraceae, Anthemideae): localisation de l'hétérochromatine et de l'ADN ribosomique. Bocconea 13: 623–628

    Google Scholar 

  • Torrell M., Cerbah M., Siljak-Yakovlev S. and Vallès J. (2003). Molecular cytogenetics of the genus Artemisia (Asteraceae, Anthemideae): fluorochrome banding and fluorescent in situ hybridization. I. Subgenus Seriphidium and related taxa. Pl. Syst. Evol. 239: 141–153

    Article  CAS  Google Scholar 

  • Trivers R., Burt A. and Palestis B. G. (2004). B-chromosomes and genome size in flowering plants. Genome 47: 1–8

    Article  PubMed  CAS  Google Scholar 

  • Vallès J. (1987). Aportación al conocimiento citotaxonómico de ocho táxones ibéricos del género Artemisia L. (Asteraceae, Anthemideae). Ann. Jard. Bot. Madrid 44: 79–96

    Google Scholar 

  • Vallès J., Garnatje T., Garcia S., Sanz M. and Korobkov A. A. (2005). Chromosome numbers in the tribes Anthemideae and Inuleae (Asteraceae). Bot. J. Linn. Soc. 148: 77–85

    Article  Google Scholar 

  • Vallès J. and Siljak-Yakovlev S. (1997). Cytogenetic studies in the genus Artemisia L.: fluorochrome banded karyotypes of five taxa, including the Iberian endemic species A. barrelieri Besser. Canad. J. Bot. 75: 595–606

    Article  Google Scholar 

  • Vallès J., Torrell M., Garnatje T., Garcia-Jacas N., Vilatersana R. and Susanna A. (2003). The genus Artemisia and its allies: phylogeny of the subtribe Artemisiinae (Asteraceae, Anthemideae) based on nucleotide sequences of nuclear ribosomal DNA internal transcribed spacers (ITS). Pl. Biol. 5: 274–284

    Article  Google Scholar 

  • Vischi M., Jurman I., Bianchi G. and Morgante M. (2003). Karyotype of Norway spruce by multicolor FISH. Theor. Appl. Genet. 107: 591–597

    Article  PubMed  CAS  Google Scholar 

  • Vitturi R., Colomba M. S., Pirrone A. M. and Mandrioli M. (2002). rDNA (18S–28S and 5S) colocalization and linkage between ribosomal genes and (TTAGGG) n telomeric sequence in the earthworm, Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae), revealed by single- and double-color FISH. J. Heredity 93: 279–282

    Article  CAS  Google Scholar 

  • Ward G. H. (1953). Artemisia section Seriphidium in North America: A cytotaxonomic study. Contributions from the Dudley Herbarium of Stanford University 4: 155–205

    Google Scholar 

  • Zoldos V., Papes D., Cerbah M., Panaud O., Besendorfer V. and Siljak-Yakovlev S. (1999). Molecular-cytogenetic studies of ribosomal genes and heterochromatin reveal conserved genome organization among 11 Quercus species. Theor. Appl. Genet. 99: 967–977

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratori de Botànica Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n 08028, Barcelona, Catalonia, Spain

    S. Garcia & J. Vallès

  2. Institut Botànic de Barcelona (CSIC-ICUB), Passeig del Migdia s/n 08038, Barcelona, Catalonia, Spain

    T. Garnatje & O. Hidalgo

  3. United States Department of Agriculture, Shrub Sciences Laboratory, Rocky Mountain Research Station, Forest Service, Provo, UT, 84606, USA

    E. D. McArthur

  4. Ecologie, Systématique, Evolution, UMR CNRS 8079, Université Paris-Sud, Bâtiment 360, 91405, Orsay Cedex, France

    S. Siljak-Yakovlev

Authors
  1. S. Garcia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Garnatje
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Hidalgo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. D. McArthur
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Siljak-Yakovlev
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. Vallès
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Garcia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Garcia, S., Garnatje, T., Hidalgo, O. et al. Extensive ribosomal DNA (18S-5.8S-26S and 5S) colocalization in the North American endemic sagebrushes (subgenus Tridentatae, Artemisia, Asteraceae) revealed by FISH. Plant Syst. Evol. 267, 79–92 (2007). https://doi.org/10.1007/s00606-007-0558-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-007-0558-6

Keywords

Search

Navigation