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Detection of alkaline phosphatase activity with a CsPbBr3/Y6 heterojunction-based photoelectrochemical sensor

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Abstract

An ultra-sensitive photoelectrochemical (PEC) sensor based on perovskite composite was developed for the determination of alkaline phosphatase (ALP) in human serum. In contrast to CsPbBr3 or Y6 that generated anodic current, the heterojunction of CsPbBr3/Y6 promoted photocarriers to separate and generated cathodic photocurrent. Ascorbic acid (AA) was produced by ALP hydrolyzing L-ascorbic acid 2-phosphate trisodium salt (AAP), which can combine with the holes on the photoelectrode surface, accelerating the transmission of photogenerated carriers, leading to enhanced photocurrent intensity. Thus, the enhancement of PEC current was linked to ALP activity. The PEC sensor exhibits good sensitivity for detection of ALP owing to the unique photoelectric properties of the CsPbBr3/Y6 heterojunction. The detection limit of the sensor was 0.012 U·L−1 with a linear dynamic range of 0.02–2000 U·L−1. Therefore, this PEC sensing platform shows great potential for the development of different PEC sensors.

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Data availability

The data that support the findings of this study are available from the corresponding author [M Yang], upon reasonable request.

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Funding

The authors are thankful for the support of this work by the Natural Science Foundation of Hunan Province (Grant No. 2023JJ70059) and the National Natural Science Foundation of China (Grant No. 22174163).

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China

    Rong Shao, Lei Deng & Minghui Yang

  2. Department of Oral and Maxillofacial Surgery, Center of Stomatology, Xiangya Hospital, Central South University, Changsha, 410083, China

    Shujun Hu & Anjie Min

  3. Research Center of Oral and Maxillofacail Tumor, Xiangya Hospital, Central South University, Changsha, 410083, China

    Shujun Hu & Anjie Min

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  1. Rong Shao
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  2. Lei Deng
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  3. Shujun Hu
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  4. Minghui Yang
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  5. Anjie Min
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Corresponding authors

Correspondence to Minghui Yang or Anjie Min.

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All experiments were in accordance with the guidelines of the National Institute of Health, China, and approved by the Institutional Ethical Committee (IEC) of Central South University.

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We also obtained informed consent for any experimentation with human serum samples.

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Shao, R., Deng, L., Hu, S. et al. Detection of alkaline phosphatase activity with a CsPbBr3/Y6 heterojunction-based photoelectrochemical sensor. Microchim Acta 191, 316 (2024). https://doi.org/10.1007/s00604-024-06393-2

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